Recombinant JC polyomavirus Large T antigen, partial

中文名稱：

Recombinant JC polyomavirus Large T antigen ,partial
貨號：

CSB-YP360948JAK
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant JC polyomavirus Large T antigen ,partial
貨號：

CSB-EP360948JAK
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant JC polyomavirus Large T antigen ,partial
貨號：

CSB-EP360948JAK-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant JC polyomavirus Large T antigen ,partial
貨號：

CSB-BP360948JAK
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant JC polyomavirus Large T antigen ,partial
貨號：

CSB-MP360948JAK
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

N/A
Uniprot No.：

P03072
別名：

Large T antigen; LT; LT-AG; EC 3.6.4.-
種屬：

JC polyomavirus (JCPyV) (JCV)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription.
基因功能參考文獻：
1. The V392G amino acid substitution in T antigen identified frequently in progressive multifocal leukoencephalopathy (PML) lesions has a function in suppressing JC virus replication, but the frequency of the mutation was restricted and its role in PML lesions was limited. PMID: 27795410
2. These findings have revealed a molecular interplay between cellular SRSF1 and viral T-antigen in controlling JC virus gene expression. PMID: 26596376
3. VP2 and VP3 enhance large T antigen binding to the origin of viral DNA replication. PMID: 24418532
4. Authors found that JC virus T antigen enhances the promoter activity of MeCP2, but does not enhance the mRNA and protein levels of MeCP2. PMID: 23514909
5. Data suggest that by investigating the interplay between large T-antigen (T-Ag) and Bag3 may provid better understanding on the development of JCV-associated diseases. PMID: 22984599
6. Data show that JC polyomavirus T-antigen inhibits homologous recombination DNA repair by binding insulin receptor substrate 1, which then translocates to the nucleus and binds Rad51 at the site of damaged DNA. PMID: 15965906
7. These observations unravel the interplay between beta-catenin and Rac1 that is initiated by T-Ag and results in stabilization of beta-catenin and its presence in cell membrane ruffles. PMID: 17637758
8. This study showed that BAG3 protein is downregulated upon JC virus (JCV) infection and that this effect is mediated by JCV T-antigen via repression of the BAG3 promoter. PMID: 19282432
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亞細胞定位：

Host nucleus.
數據庫鏈接：

KEGG: vg:1489517