Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev)

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中文名稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev)
品名簡稱：

Recombinant Human immunodeficiency virus type 1 group M subtype B rev protein
貨號：

CSB-EP356309HKP
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

rev
Uniprot No.：

P04618
種屬：

Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)
蛋白長度：

Full Length
來源：

E.coli
分子量：

20.0 kDa
表達區(qū)域：

1-116aa
氨基酸序列

MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILGTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm.
基因功能參考文獻：
1. These results suggest a differential interaction between human Crm1 and mouse Crm1 and many lentiviral Rev proteins, which may partially explain the HIV replicative defect in mice. PMID: 29028476
2. DDX1 binds HIV-1 rev response element to promote virus assembly. PMID: 28379444
3. Results indicate that the binding between ctDNA and REV peptide is a specific electrostatic interaction governed by oppositely charged molecules. PMID: 27353011
4. Results support the model that proper folding of Rev is critical in ensuring that the flexible Rev-response element is able to correctly position Rev molecules for specific ribonucleoprotein assembly. PMID: 26620599
5. Nucleophosmin Interactions with Human Immunodeficiency Virus Rev and Herpes Simplex Virus US11 PMID: 26624888
6. The DEAG-box of human MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export. PMID: 26379090
7. Posttranscriptional regulatory Rev protein function and HIV-1 replication in astrocytes proceed unperturbed. PMID: 25188302
8. Sequestration od DDX1 led to destabilization of the Rev nucleocytoplasmic transport complex and decreased levels of Rev-dependent viral transcripts. PMID: 25496916
9. RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev-Rev response element complex. PMID: 25486594
10. This study identifies a cellular protein named RBM14 that is associated with XPO1 (CRM1), a nuclear protein that binds to the HIV-1 Rev protein and mediates nuclear export of incompletely spliced HIV-1 viral RNAs PMID: 25589658
11. RNA-guided assembly of Rev-Rev response element nuclear export complexes has been described. PMID: 25163983
12. These results suggest a model wherein HIV-1 Rev-associated nuclear export signals cooperate to regulate the number or quality of CRM1's interactions with viral Rev/RRE ribonucleoprotein complexes in the nucleus. PMID: 25275125
13. Authors establish that human Staufen-2, a host factor which is up-regulated upon HIV-1 infection, interacts with HIV-1 Rev, thereby promoting its RNA export activity and progeny virus formation. PMID: 24520823
14. HIV-1 Rev's arginine-rich RNA-binding motif is intrinsically disordered; RRE recognition triggers a crucial coil-to-helix transition employing an induced-fit mechanism PMID: 23972852
15. The viral Rev response element (RRE) adopts an "A"-like structure in which the two legs constitute two tracks of binding sites for the viral Rev protein and position the two primary known Rev-binding sites ~55 A apart, matching the distance between the two RNA-binding motifs in the Rev dimer. PMID: 24243017
16. Rather than acting as a simple on-off switch or maintaining a constant level of activity throughout infection, the Rev-Rev response element system can fluctuate, presumably to control replication. PMID: 23926352
17. Most Rev arginine-rich motif residues tolerated substitutions without strong loss of binding to Rev response element IIB. PMID: 23595810
18. Results suggest that distinct DDX DEAD-box RNA helicases DDX3 and DDX5 cooperate to modulate the HIV-1 Rev function. PMID: 23608157
19. HIV-1 Rev protein enhances encapsidation of unspliced and spliced, RRE-containing lentiviral vector RNA PMID: 23133650
20. PSF and MATR3 are cellular host factors that bind viral RNA and promote Rev activity. PMID: 23158102
21. Rev oligomerization on the Rev Response Element, thereby recruiting at least two protein activation domains, is required for nuclear export of unspliced and incompletely spliced viral RNA. PMID: 22675540
22. DDX1 was shown to be an RNA-activated ATPase, wherein Rev-bound RNA was equally effective at stimulating ATPase activity as protein-free RNA. PMID: 22051512
23. we hypothesize that CRM1 was subject to strong selection event in primate lineage ~80 million years ago that altered sequence of HEAT repeat 9A. Resulting CRM1 sequence is better able to support HIV-1 Rev's function as mediator of viral RNA nuclear export PMID: 22114565
24. Rev dimerization and oligomerization interfaces to a resolution of 2.5A. PMID: 21941623
25. HIV-1 Rev protein activates HCV gene expression PMID: 22094166
26. Rev protein of Human Immunodeficiency Virus type 1 (HIV-1) inhibits nuclear import and mediates nuclear export of the HIV-1 integrase (IN) protein PMID: 21326951
27. The position of the charges in aminoglycoside antibiotics and the flexibility of their structure, rather than the number of charges, determines whether an aminoglycoside antibiotic will be able to bind with high affinity to TAR RNA. PMID: 21916409
28. Matrin 3 binds Rev RNA to stabilize HIV-1 transcripts leading to increased cytoplasmic expression. PMID: 21771347
29. Rev needs MATR3 to promote the cytoplasmic accumulation and translation of unspliced RRE-containing mRNA. PMID: 21771348
30. MATR3 binds viral RNA and is required for the Rev/RRE mediated nuclear export of unspliced HIV-1 RNAs. PMID: 21771346
31. The authors show for the first time that the Rev/RRE system can augment RNA encapsidation independent of all cis elements from the 5' UTR (R, U5, PBS, and psi). PMID: 21702950
32. DDX1 acts as a cellular cofactor by promoting oligomerization of Rev on the Rev response element. PMID: 21763499
33. The appropriate intracellular localization and dynamics of Rev could regulate Gag assembly and HIV-1 replication. PMID: 21364984
34. RanBP2 is a host factor that is involved in the nuclear import of HIV-1 PIC (DNA), but is not critical to the nuclear export of the viral mRNAs or nucleo-cytoplasmic shuttling of Rev PMID: 21179483
35. We demonstrate that HIC selectively interferes with Rev NLS interaction with importin beta and impedes its nuclear import and function, but does not affect Rev nuclear import mediated by transportin. PMID: 21401918
36. an intrabody based on a llama single-domain antibody targeting the N-terminal alpha-helical multimerization domain of HIV-1 rev prevents viral production PMID: 20406803
37. These results probably indicate that binding of excess amounts of the human immunodeficiency virus type 1 Rev protein or the NES peptide to the human exportin receptor CRM1 results in cells death. PMID: 20151187
38. Rev enhanced cytoplasmic levels of the unspliced HIV-1 RNA, mostly 3- to 12-fold, while encapsidation of the RNA and viral infectivity could be stimulated >1,000-fold. PMID: 20427541
39. The authors conclude that superinfection resistance involves Rev-dependent control of human immunodeficiency virus cDNA integration. PMID: 20147519
40. Results show that HIV-1 Rev promotes dissociation of the IN-LEDGF/p75 complex. Combination of the viral IN and the cellular LEDGF/p75 is required for proper integration of the viral cDNA into the host chromosomal DNA. PMID: 19855849
41. Data show that the interaction of HIV-1 Rev with functionally diverse hnRNPs lends support that Rev is a multifunctional protein and may be involved in coupling HIV replication to diverse cellular processes and promoting virus-host cell interactions. PMID: 19808671
42. HIV-1 Rev transactivator: a beta-subunit directed substrate and effector of protein kinase CK2 PMID: 11827166
43. Physiologically significant interaction of hNup98 with HIV-1 Rev PMID: 14554087
44. SF2/ASF and SRp40 activate the ESE (exonic splicing enhancer) which regulates the expression of HIV-1 rev, env, vpu, and nef PMID: 15163745
45. These findings indicate that DDX1 is a critical cellular co-factor for Rev function, which maintains the proper subcellular distribution of this lentiviral regulatory protein. PMID: 15567440
46. the effect of Rev on env mRNA transport is low compared with the enhancement of translation of this mRNA PMID: 15777852
47. All trimerization-defective Rev molecules had profoundly compromised Rev function and a range of localization defects was observed. PMID: 15831964
48. Yeast-two hybrid screening of a T-cell cDNA library with Rev as bait led to isolation of a novel human cDNA product (16.4.1); results suggest that 16.4.1 may act as a modulator of Rev activity. PMID: 15847701
49. The backbone cyclic peptide Rev-13 analogue inhibited Rev-induced gene expression in HeLa cells. PMID: 16114892
50. Data suggest that steady-state subcellular localization is not a primary regulator of Rev activity but may change as a secondary consequence of altered Rev function. PMID: 16368434
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亞細(xì)胞定位：

Host nucleus, host nucleolus. Host cytoplasm.
蛋白家族：

HIV-1 REV protein family
數(shù)據(jù)庫鏈接：

KEGG: vg:155908