Recombinant Human herpesvirus 1 ICP47 protein (US12)

中文名稱：

Recombinant Human herpesvirus 1 ICP47 protein(US12)
貨號：

CSB-YP360979HWY
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

US12
Uniprot No.：

P03170
別名：

US12; ICP47 protein; Immediate-early protein IE12; Immediate-early-5; Infected cell protein 47; US12 protein; Vmw12
種屬：

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
蛋白長度：

Full Length
來源：

Yeast
分子量：

11.8kDa
表達區域：

1-88aa
氨基酸序列

MSWALEMADTFLDTMRVGPRTYADVRDEINKRGREDREAARTAVHDPERPLLRSPGLLPEIAPNASLGVAHRRTGGTVTDSPRNPVTR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The fusion tag N-terminal 6xHis tag gene was added to the gene sequence corresponding to the yeast of the HHV-1 US12 protein to form the recombinant DNA. The recombinant DNA was cloned into the expression vector and then transfected into the yeast cells for expression. Following purification, the product is the recombinant HHV-1 US12 protein carrying N-terminal 6xHis tag. The SDS-PAGE assessed the purity of this recombinant US12 protein up to 90%. It had an apparent molecular weight of approximately 14 kDa.
US12 is gene providing instructions for making a protein named human herpesvirus 1 ICP47 protein (abbreviated US12) and belongs to Herpesviridae US12 family. US12, a HSV immediate early protein, is small protein produced by the herpes simplex virus, is considered as an important factor in the evasion of cellular immune responses in HSV-infected cells. It is expressed very early after infection and can inhibit CD8+ T cell recognition of infected cells. Recent studies have established clearly that ICP47 can bind with high affinity to human TAP and inhibit the transport of peptides into the endoplasmic reticulum.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a role in the inhibition of host immune response. Binds specifically to transporters associated with antigen processing (TAP), thereby blocking peptide-binding and translocation by TAP as well as subsequent loading of peptides onto MHC class I molecules. Empty MHC I molecules are retained in the endoplasmic reticulum and ultimately directed to proteasomal degradation. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.
基因功能參考文獻：
1. The structure shows that herpes simplex virus ICP47 traps human TAP in an inactive conformation distinct from the normal transport cycle. PMID: 27935481
2. cryo-electron microscopy structure of human TAP in complex with its inhibitor ICP47, a small protein produced by the herpes simplex virus I PMID: 26789246
3. Results indicate that recombinant adenovirus r-H-ICP47 had the abilities of reducing the proliferation of lymphocytes activated by dendritic cells (DCs). PMID: 23774531
亞細胞定位：

Host cytoplasm. Host nucleus.
蛋白家族：

Herpesviridae US12 family
數據庫鏈接：

KEGG: vg:2703441