Recombinant Human Zinc finger protein Gfi-1b (GFI1B)

中文名稱：

人GFI1B重組蛋白
貨號：

CSB-YP733153HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人GFI1B重組蛋白
貨號：

CSB-EP733153HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人GFI1B重組蛋白
貨號：

CSB-EP733153HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人GFI1B重組蛋白
貨號：

CSB-BP733153HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人GFI1B重組蛋白
貨號：

CSB-MP733153HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

GFI1B
Uniprot No.：

Q5VTD9
別名：

GFI 1B; gfi1b; GFI1B protein; GFI1B_HUMAN; Growth factor independent 1B protein; Growth factor independent 1B transcription repressor; Growth factor independent protein 1B; OTTHUMP00000022443; OTTHUMP00000022444; OTTHUMP000000235527; Potential regulator of CDKN1A; Potential regulator of CDKN1A translocated in CML; Translocated in CML; Zinc finger protein Gfi-1b
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-330
氨基酸序列

MPRSFLVKSK KAHTYHQPRV QEDEPLWPPA LTPVPRDQAP SNSPVLSTLF PNQCLDWTNL KREPELEQDQ NLARMAPAPE GPIVLSRPQD GDSPLSDSPP FYKPSFSWDT LATTYGHSYR QAPSTMQSAF LEHSVSLYGS PLVPSTEPAL DFSLRYSPGM DAYHCVKCNK VFSTPHGLEV HVRRSHSGTR PFACDICGKT FGHAVSLEQH THVHSQERSF ECRMCGKAFK RSSTLSTHLL IHSDTRPYPC QFCGKRFHQK SDMKKHTYIH TGEKPHKCQV CGKAFSQSSN LITHSRKHTG FKPFSCELCT KGFQRKVDLR RHRESQHNLK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
基因功能參考文獻：
1. Disruption of GFI1B non-DNA-binding zinc-finger 1 is associated with mild to moderate thrombocytopenia without alpha-granule deficiency or bleeding symptomatology, indicating that the site of GFI1B mutation has important phenotypic implications. Platelet CD34 expression appears to be a common feature of perturbed GFI1B function. PMID: 28880435
2. Platelet CD34 expression and alpha/delta-granule abnormalities in GFI1B- and RUNX1-related familial bleeding disorders. PMID: 28096094
3. GFI1B is an essential protein for the normal development of the megakaryocyte lineage PMID: 27122003
4. High GFI1B expression is associated with small-cell lung cancer. PMID: 28667074
5. Results demonstrate that alpha-delta platelet storage pool deficiency is associated with either a heterozygous mutation in GFI1B (de novo or familial) abrogating the binding of the zinc fingers with the promoter of its target genes, or by hypomorphic biallelic mutations leading to autosomal recessive inheritance. PMID: 28041820
6. study demonstrates the significance of Gfi1b regulated Kindlin3-Talin1 expression in driving megakaryocytic differentiation and highlights the contribution of cytoskeletal agents in the developmental progression of these platelet progenitors PMID: 27768697
7. Role for Alternative GFI1B Splice Variants in Human Hematopoiesis PMID: 27486782
8. SPI1-GFI1B transcriptional network is an important regulatory axis in acute myeloid leukemia as well as in the development of erythroid versus myelomonocytic cell fate PMID: 26851695
9. Gfi1b functions as a transcriptional repressor by recruiting histone-modifying enzymes to promoters and enhancers of target genes. Mutations are associated with certain bleeding disorders. Review. PMID: 26447191
10. a mutation in GFI1B causes a platelet disorder; GFI1B has a role a critical regulator of platelet shape, number, and function PMID: 23927492
11. results, supported by evidence from mouse models, identify GFI1 and GFI1B as prominent medulloblastoma oncogenes and implicate 'enhancer hijacking' as an efficient mechanism driving oncogene activation in a childhood cancer PMID: 25043047
12. TAL1 is involved in regulating H3K27me3 variations in collaboration with GFI1B PMID: 24395799
13. Silencing of both BCR-ABL siRNA and GFI1B siRNA is associated with an additive antileukemic effect against K562 cells. PMID: 23788109
14. Our studies show that GFI1B, in addition to being causally related to the gray platelet syndrome, is key to megakaryocyte and platelet development. PMID: 24325358
15. Gfi-1B p32, a Gfi-1B isoform, is essential for erythroid differentiation PMID: 22399799
16. GFI1B regulatory elements behave as activators and repressors, consistent with a model in which GFI1B binds to its own promoter and to the conserved non-coding elements as its levels rise. PMID: 19773260
17. Results reveal the presence of different protein complexes, including GATA-1 and Oct-1, involved in Gfi1b regulation. PMID: 20143233
18. repressing TGF-beta type III receptor (TbetaRIotaII) expression, Gfi-1B favors the Smad2/TIF1-gamma interaction downstream of TGF-beta signaling, allowing immature progenitors to differentiate toward the erythroid lineage. PMID: 20124515
19. HMGB2 potentiates GATA-1-dependent transcription of GFI1B by Oct-1 and thereby controls erythroid differentiation. PMID: 19965638
20. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis PMID: 12351384
21. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. PMID: 15280509
22. Gfi-1B acts in the late stage of erythroid differentiation as a transcriptional repressor. PMID: 15507521
23. GATA-1 and Gfi-1B participates in a feedback regulatory pathway in controlling the expression of the Gfi-1B gene. PMID: 16177182
24. E2A proteins prevent lymphoma cell expansion, at least in part through regulation of Gfi1b and modulation of Gata3 expression. PMID: 17272506
25. GATA1 and GFI1B interplay to regulate bcl-X protein transcription. PMID: 17420275
26. suggest that Gfi1b may be an important factor to establish or maintain myeloid leukemia and myeloproliferative diseases PMID: 19360458
27. Data conclude that Gfi-1B behaves as a lineage-affiliated gene with an open chromatin configuration in multipotent progenitors and sustained activation as cells progress throughout erythroid differentiation. PMID: 19522008
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相關疾病：

Bleeding disorder, platelet-type 17 (BDPLT17)
亞細胞定位：

Nucleus.
組織特異性：

Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocy
數據庫鏈接：

HGNC: 4238

OMIM: 187900

KEGG: hsa:8328

STRING: 9606.ENSP00000344782

UniGene: Hs.553160