Recombinant Human Villin-1 (VIL1), partial

中文名稱：

人VIL1重組蛋白
貨號：

CSB-YP025855HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人VIL1重組蛋白
貨號：

CSB-EP025855HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人VIL1重組蛋白
貨號：

CSB-EP025855HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人VIL1重組蛋白
貨號：

CSB-BP025855HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人VIL1重組蛋白
貨號：

CSB-MP025855HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

VIL1
Uniprot No.：

P09327
別名：

D2S1471; OTTHUMP00000164145; VIL; VIL1; VILI_HUMAN; Villin 1; Villin-1; Villin1
種屬：

Homo sapiens (Human)
蛋白長度：

partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
基因功能參考文獻：
1. Villin, Pro-Ex-C and progesterone/estrogen receptor expression have diagnostic and predictive roles in endocervical and endometrioid adenocarcinoma. PMID: 28832070
2. Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
3. Villin 1 is released in plasma during acute kidney injury and shows potential as an early marker for proximal tubular injury/necrosis. PMID: 28704336
4. Villin has a higher positive expression in Primary Pulmonary Enteric Adenocarcinoma cases. Villin proved to be a very sensitive and helpful enteric marker in the differential diagnosis between PEAC and other lung adenocarcinomas. PMID: 28953659
5. villin directly interacts with a transcriptional corepressor and ligand of the Slug promoter, ZBRK1. PMID: 26658611
6. Data indicate an association between highly fucosylated glycans and caudal type homeobox 1 (CDX1) and/or villin mRNA expression that both correlate with cell differentiation. PMID: 26537799
7. study demonstrates that G. duodenalis-mediated disruption of villin is, at least, in part dependent on activation of myosin light chain kinase PMID: 26334299
8. Thus, villin plays an important role in establishing the balance between actin polymerization and actin severing to facilitate the initial steps of Salmonella entry. PMID: 25600187
9. Collective expression pattern of tensin/profilin-1/villin-1/talin could be a biomarker to estimate the prognosis of esophageal squamous cell carcinoma patients. PMID: 25337239
10. harmonin and villin autoantibodies are sensitive and specific markers of IPEX, differentiate IPEX, including atypical cases, from other early childhood disorders associated with enteropathy PMID: 24250806
11. villin immunohistochemistry is a reliable and superior adjunct in the diagnosis of microvillus inclusion disease PMID: 25517957
12. Discriminated small-cell lung carcinoma from large-cell neuroendocrine lung carcinoma with immunohistochemical markers BAI3, CDX2 and VIL1. PMID: 24266897
13. We demonstrated that the VIL1 gene is a potential candidate molecular marker for high serum AFP-associated HCC and a predictive candidate for the postoperative recurrence and poorer prognosis of HCC. PMID: 22530999
14. study confirmed finding of VIL1 as a diagnostic marker of cervical adenocarcinoma PMID: 21623163
15. identified villin, an actin-binding protein, as the 95 kDa antigen in four of five sera from patients with IPEX syndrome PMID: 21741320
16. identified VIL1 as a novel biomarker of cervical adenocarcinoma PMID: 19377296
17. villin has calcium-sensitive actin-capping and actin-depolymerizing sites PMID: 15272027
18. tyrosine phosphorylation at residues 60, 81, and 256 of human villin plays an essential role in cell migration as well as in the reorganization of the actin cytoskeleton PMID: 15342783
19. H. pylori induction of villin in the stomach correlates with activation and cooperative binding of Elk-1 and the SRF to the proximal promoter of villin PMID: 15576363
20. By severing actin filaments and capping their barbed ends, villin increases the concentration of actin monomers available for polymerization. PMID: 17182858
21. Together, our results show that in epithelial cells ATX and LPA act as strong stimulators of cell migration by recruiting PLC-gamma 1 and villin, both of which participate in the initiation of protrusion. PMID: 18054784
22. Villin is an actin regulatory protein that organizes, integrates and regulates multiple epithelial cell functions. PMID: 18307996
23. Cdx2 regulates intestinal villin expression through recruiting Brm-type SWI/SNF complex to the villin promoter. PMID: 19371634
24. Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton PMID: 11500485
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相關疾病：

Biliary atresia is a chronic and progressive cholestatic liver disease of chilhood characterized by an abnormal villin gene expression and severe malformation of canalicular microvillus structure.
亞細胞定位：

Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip. Cell projection, filopodium.
蛋白家族：

Villin/gelsolin family
組織特異性：

Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).
數(shù)據(jù)庫鏈接：

HGNC: 12690

OMIM: 193040

KEGG: hsa:7429

STRING: 9606.ENSP00000248444

UniGene: Hs.654595