Recombinant Human Transcription factor EB (TFEB)

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中文名稱：

Recombinant Human Transcription factor EB (TFEB)
品名簡稱：

Recombinant Human TFEB protein
貨號：

CSB-EP023429HUc7
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

TFEB
Uniprot No.：

P19484
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

59.8 kDa
表達(dá)區(qū)域：

1-476aa
氨基酸序列

MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Involved in the immune response to infection by the bacteria S.aureus or S.enterica, acting downstream of protein kinase D (PKD), probably by regulating cytokine and chemokine expression.
基因功能參考文獻(xiàn)：
1. TMEM106B drives lung cancer metastasis by inducing TFEB-dependent lysosome synthesis and secretion of cathepsins. PMID: 30013069
2. data on TFEB nucleo-cytoplasmic shuttling suggest an unpredicted role of mTOR in nuclear export. PMID: 30120233
3. The transcription factor EB (TFEB) nuclear export signal (NES) integrates carbon (glucose) and nitrogen (amino acid) availability by controlling TFEB flux through a nuclear import-export cycle. PMID: 29992949
4. TFEB knockdown reduces invasion and migration of cancer cells, likely through lysosomal regulation. Taken together, TFEB influences cell invasion and migration of oral squamous cell carcinomas. PMID: 29316035
5. Akt phosphorylates TFEB at Ser467 and represses TFEB nuclear translocation independently of mechanistic target of rapamycin complex 1, a known TFEB inhibitor. Pharmacological inhibition of Akt promotes cellular clearance in cells from patients with a variety of lysosomal diseases. PMID: 28165011
6. these data suggest that the TFEB/TMEM55B/JIP4 pathway coordinates lysosome movement in response to a variety of stress conditions. PMID: 29146937
7. Data suggest that the effects of Alpha-TFEB gene fusion are specific in renal cell carcinoma, which results in the overexpression of a native TFEB protein and then promotes cell canceration. PMID: 29328409
8. MAP4K3 is identified as an amino acid-dependent regulator of autophagy through its phosphorylation of transcription factor EB (TFEB), a transcriptional activator of autophagy. PMID: 29507340
9. Overexpression of TFEB is associated with Breast Cancer. PMID: 28017540
10. Results identify cigarette smoke (CS)-induced accumulation of TFEB in aggresome-bodies as a specific novel mechanism for CS-mediated autophagy-impairment and resulting aggresome-formation and emphysema progression. PMID: 27835930
11. This study reveals a critical link between two keys factors in tumourigenesis and autophagy, and suggests a potential important role of p53-TFEB signaling axis in lung cancer. PMID: 28292013
12. Taken together, these data demonstrate that ATO induces osteosarcoma cell death via inducing excessive autophagy, which is mediated through the ROS-TFEB pathway. The present study provides a new anti-tumor mechanism of ATO treatment in osteosarcoma. PMID: 29307831
13. cellular vacuolization, represents a condition of profound lysosome stress, and cells sense and respond to this stress by facilitating mTOR-dependent TFEB nucleus translocation in an effort to restore lysosome homeostasis. PMID: 27593892
14. TFEB and TFE3 collaborate with each other in activated macrophages and microglia to promote efficient autophagy induction, increased lysosomal biogenesis, and transcriptional upregulation of numerous proinflammatory cytokines PMID: 27171064
15. TFEB is affected by a novel curcumin analog in vitro and in vivo independent of MTOR inhibition PMID: 27172265
16. TFEB regulates PER3 expression via glucose-dependent effects on CLOCK/BMAL1 PMID: 27373683
17. These data reveal a novel mechanism of TFEB regulation by MTORC1 essential for lysosomal biogenesis. PMID: 28055300
18. Data suggest that transcription factor EB (TFEB) is a protective transcription factor against endothelial cell inflammation and a potential target for treating atherosclerosis and associated cardiovascular diseases. PMID: 28143903
19. Both Danon Disease and glycogen storage disease type II show accumulation and altered localization of VPS15 in autophagy-incompetent fibers. However, TFEB displays a different pattern between these two lysosomal storage diseases PMID: 28102838
20. Studies identified TFEB and TFE3 as master modulators of stress response notably in the lysosomal biogenesis and autophagy with capability to upregulate hundreds of genes involved in intracellular clearance, catabolism, metabolic processes, and cellular homeostasis. PMID: 27892768
21. Findings suggest that TFEB activation in tauopathy model mice stimulates the autophagy-lysosome pathway, resulting in the clearance of PHF-tau and lipofuscins, which in turn may rescue loss of synapses, and learning and memory deficits. PMID: 27257626
22. Data suggest that PEG3 is required for TFEB induction and nuclear translocation in a VEGFR2- and AMPK-dependent manner for decorin/decorin receptor-evoked autophagy. (PEG3 = paternally expressed 3 protein; TFEB = transcription factor EB; VEGFR2 = vascular endothelial growth factor receptor-2; AMPK = AMP-activated protein kinase) PMID: 28798237
23. review of role linking cellular stress to immune response PMID: 28656016
24. Results demonstrate that TFEB levels and subcellular distribution undergo distinct short-term and long-term control. These findings suggest that the rapid rheostatic response, mediated by mTOR, allows the cell to quickly adapt to metabolic changes, while the long-term, mTOR independent homeostatic response controls the magnitude and duration of TFEB activation, and presumably limits excessive autophagy. PMID: 27268034
25. Consistent with reduced transcription factor EB (TFEB) activity, accumulation of phosphorylated TFEB in STUB1-deficient cells resulted in reduced autophagy and reduced mitochondrial biogenesis. These studies reveal that the ubiquitin-proteasome pathway participates in regulating autophagy and lysosomal functions by regulating the activity of TFEB. PMID: 28754656
26. TFEB has attracted a lot of attention owing to its ability to induce the intracellular clearance of pathogenic factors in a variety of murine models of disease, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity. PMID: 27252382
27. Amplification of chromosome 6p including the TFEB gene is a novel occurrence in renal cell carcinoma, which seems to be associated with an often aggressive and infiltrative tubulopapillary growth pattern. PMID: 28009604
28. TFEB-amplified renal cell carcinomas represent a distinct molecular subtype of high-grade adult renal cell carcinomas associated with aggressive clinical behavior, variable morphology, and aberrant melanocytic marker expression PMID: 27565001
29. Case Report: suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma. PMID: 27864122
30. Overexpression of deacetylated transcription factor EB at K116R mutant in microglia accelerated intracellular fibrillar Amyloid beta-peptide degradation by stimulating lysosomal biogenesis and greatly reduced the deposited amyloid plaques in the brain. PMID: 27209302
31. the central autophagy regulator TFEB is expressed and active in PDAC, but autophagy is sustained after TFEB knockdown, suggesting alternative bypass signaling. TFEB is dispensable for gemcitabine-induced cell death, but inversely correlated with KRAS expression. PMID: 27175909
32. Neuronal C-ETS2 senses oxidative stress, activates TFEB transcription, and mediates the upregulation of lysosomal genes. PMID: 27195074
33. TFEB is phosphorylated and inactivated by Akt. PMID: 28165011
34. Activation of TFEB ameliorates disease phenotypes in a mouse model of Batten disease. PMID: 28165011
35. Lack of cystinosin reduced TFEB expression and induced TFEB nuclear translocation. PMID: 26994576
36. The autophagic response to polystyrene nanoparticles is mediated by TFEB and depends on surface charge. PMID: 26596266
37. TFEB1 overexpression is associated with drug resistance of ovarian cancer. PMID: 26307679
38. Review of the role of TFEB in lysosome biogenesis, transcriptional and post-transcriptional regulation of TFEB, and TFEB involvement in neurodegenerative disease as a dysregulated factor or as an agent used to promote cellular clearance. PMID: 27299292
39. TFEB and TFE3 are novel components of the integrated stress response PMID: 26813791
40. Silencing of TFEB with siRNAs in lung cancer cell lines resulted in reduced migration ability. PMID: 26264650
41. during mitophagy TFEB translocates to the nucleus and displays transcriptional activity in a PINK1- and Parkin-dependent manner. PMID: 26240184
42. a virus modulating TFEB localization and helps to explain how HIV modulates autophagy to promote its own replication and cell survival PMID: 26115100
43. RIP1 represses basal autophagy in part due to its ability to regulate the TFEB transcription factor;RIP1 activates ERK, which negatively regulates TFEB though phosphorylation of serine 142 PMID: 25908842
44. TFEB modulates autophagic clearance of alpha-syn PMID: 25790376
45. Data show that drug-induced TFEB-associated lysosomal biogenesis is a determinant of multidrug resistance (MDR) and suggest that circumvention of lysosomal drug sequestration is a strategy to overcome chemoresistance. PMID: 25544758
46. This study demonstrated that transcription factor EB (TFEB) regulate the lysosome biogenesis in neurons of APP/PS1 mice, steady-state levels of APP were reduced, resulting in decreased interstitial fluid Abeta levels and attenuated amyloid deposits PMID: 26338325
47. TFEB was found to regulate MuRF1 expression in Angiotensin II-induced skeletal muscle atrophy. PMID: 26137861
48. Review of the role of gene fusions involving TFE3 and TFEB in carcinogenesis in sporadic renal cell carcinoma. PMID: 25048860
49. Results showed the amplification of TFEB locus was found only in the aggressive t(6;11) Renal Cell Carcinoma. PMID: 25438924
50. Lysosomal calcium signaling regulates autophagy through calcineurin and TFEB. PMID: 25720963
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亞細(xì)胞定位：

Cytoplasm, cytosol. Lysosome membrane. Nucleus.; Nucleus.
蛋白家族：

MiT/TFE family
數(shù)據(jù)庫鏈接：

HGNC: 11753

OMIM: 600744

KEGG: hsa:7942

STRING: 9606.ENSP00000230323

UniGene: Hs.485360