Recombinant Human Terminal uridylyltransferase 4 (ZCCHC11), partial

中文名稱：

人ZCCHC11重組蛋白
貨號：

CSB-YP719419HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人ZCCHC11重組蛋白
貨號：

CSB-EP719419HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人ZCCHC11重組蛋白
貨號：

CSB-EP719419HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人ZCCHC11重組蛋白
貨號：

CSB-BP719419HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人ZCCHC11重組蛋白
貨號：

CSB-MP719419HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ZCCHC11
Uniprot No.：

Q5TAX3
別名：

DKFZp779C1943; EC 2.7.7.52; FLJ42878; KIAA0191; PAP associated domain containing 3; PAPD3; Terminal uridylyltransferase 4; TUT4; TUT4_HUMAN; TUTase 4; ZCCHC 11; Zcchc11; Zinc finger CCHC domain containing 11; Zinc finger CCHC domain containing protein 11; Zinc finger CCHC domain-containing protein 11
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesis using 3 different uridylation mechanisms. Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression. In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing. Adds oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species. May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult. TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules.
基因功能參考文獻：
1. The authors found that TUT4(7) utilize two multidomain functional modules during the switch from either promoting expression of let-7 microRNA (monoU) or marking it for degradation (oligoU). PMID: 28671666
2. For overall breast cancer risk, three single-nucleotide polymorphisms (SNPs) in miRNA biogenesis genes DROSHA rs78393591 (OR = 0.69, 95 % CI: 0.55-0.88, P = 0.003), ESR1 rs523736 (OR = 0.88, 95 % CI: 0.82-0.95, P = 3.99 x 10(-4)), and ZCCHC11 rs114101502 (OR = 1.33, 95 % CI: 1.11-1.59, P = 0.002), and one SNP in primary miRNA sequence (rs116159732 in miR-6826, OR = 0.74, 95 % CI: 0.63-0.89, P = 0.001) were found to have s PMID: 27380242
3. Data indicate that some of the small molecules were validated as specific inhibitors of 3' terminal uridylyl transferase (TUTase) Zcchc11 (TUT4) activity. PMID: 26114892
4. Study identified TUT4 and TUT7 as uridylyl transferases for poly(A)+ mRNAs in humans and delineated in detail the action mechanism and molecular function of uridylation in the mRNA decay pathway. PMID: 25480299
5. miR-26a directly targets Lin28B and Zcchc11-two critical repressors of let-7 maturation. PMID: 24056962
6. Study identified TUT7, TUT4, and TUT2 as novel components of the miRNA biogenesis pathway. PMID: 23063654
7. Lin28 uses two different TUTases to control let-7 expression . PMID: 22898984
8. Terminal uridyltransferase enzyme Zcchc11 promotes cell proliferation independent of its uridyltransferase activity PMID: 22006926
9. This study uncovers the role of TUT4 and Lin28 as specific suppressors of microRNA biogenesis, which has implications for stem cell research and cancer biology. PMID: 19703396
10. We propose that ZCCHC11 is a unique TLR signal regulator, which interacts with TIFA after LPS treatment and suppresses the TRAF6-dependent activation of NF-kappaB. PMID: 16643855
11. Zcchc11 fine tunes IL-6 production by uridylating miR-26a. PMID: 19701194
12. TUT4 adds an oligouridine tail to the pre-let-7, which blocks Dicer processing. Knockdown of TUT4 and Lin28 reduces the level of stem cell markers PMID: 19703396
顯示更多

收起更多
亞細胞定位：

Nucleus. Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule.
蛋白家族：

DNA polymerase type-B-like family
數據庫鏈接：

HGNC: 28981

OMIM: 613692

KEGG: hsa:23318

STRING: 9606.ENSP00000257177

UniGene: Hs.655407