Recombinant Human TRAF-interacting protein (TRAIP)

中文名稱：

人TRAIP重組蛋白
貨號：

CSB-YP866296HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人TRAIP重組蛋白
貨號：

CSB-EP866296HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人TRAIP重組蛋白
貨號：

CSB-MP866296HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

TRAIP
Uniprot No.：

Q9BWF2
別名：

RING finger protein 206; RNF206; TRAF Interacting Protein; TRAF-interacting protein; Traip; TRAIP_HUMAN; TRIP
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-469
氨基酸序列

MPIRALCTIC SDFFDHSRDV AAIHCGHTFH LQCLIQWFET APSRTCPQCR IQVGKRTIIN KLFFDLAQEE ENVLDAEFLK NELDNVRAQL SQKDKEKRDS QVIIDTLRDT LEERNATVVS LQQALGKAEM LCSTLKKQMK YLEQQQDETK QAQEEARRLR SKMKTMEQIE LLLQSQRPEV EEMIRDMGVG QSAVEQLAVY CVSLKKEYEN LKEARKASGE VADKLRKDLF SSRSKLQTVY SELDQAKLEL KSAQKDLQSA DKEIMSLKKK LTMLQETLNL PPVASETVDR LVLESPAPVE VNLKLRRPSF RDDIDLNATF DVDTPPARPS SSQHGYYEKL CLEKSHSPIQ DVPKKICKGP RKESQLSLGG QSCAGEPDEE LVGAFPIFVR NAILGQKQPK RPRSESSCSK DVVRTGFDGL GGRTKFIQPT DTVMIRPLPV KPKTKVKQRV RVKTVPSLFQ AKLDTFLWS
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

E3 ubiquitin ligase required to protect genome stability in response to replication stress. Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription. Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway. Only catalyzes ubiquitination of MCM7 when forks converge. Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: promotes ubiquitination of DPCs, leading to their degradation by the proteasome. Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity. The function in translesion synthesis is however controversial. Acts as a regulator of the spindle assembly checkpoint. Also acts as a negative regulator of innate immune signaling by inhibiting activation of NF-kappa-B mediated by TNF. Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation.
基因功能參考文獻(xiàn)：
1. The TRAIP coiled-coil domain altered its stoichiometry between dimer and trimer in a concentration-dependent manner. Additionally, the TRAIP RING domain induced even higher-ordered assembly, which was necessary for interacting with the TRAF-N domain of TRAF2 but not TRAF1. PMID: 30127245
2. TRIP interacts with transforming growth factor beta-activated kinase 1 (TAK1) and promotes K48-linked polyubiquitination of TAK1 in rheumatoid arthritis fibroblast-Like synoviocytes PMID: 27847407
3. Taken together, these findings improve the understanding clinical implication of TRAIP in various diseases including primordial dwarfism and cancers. PMID: 26820530
4. cell cycle-dependent transcription of the TRAIP gene by E2F1, E2F2, and E2F4 and rapid protein degradation leads to cell cycle-dependent expression with a maximum in G2/M PMID: 26369285
5. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.( PMID: 26781088
6. These findings establish TRAIP as a PCNA-binding ubiquitin ligase with an important role in protecting genome integrity after obstacles to DNA replication. PMID: 26711499
7. TRAIP is a component of the DNA damage response to replication-blocking DNA lesions.TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. PMID: 26595769
8. The TRAIP ubiquitin ligase activity is functionally required for the spindle assembly checkpoint control. PMID: 25335891
9. a number of TRAIP mutants were used to define the TRAIP molecular domains responsible for its homo-dimerization. A co-immunoprecipitation assay indicated that the TRAIP forms homo-dimerization through the CC domain PMID: 26093298
10. Data indicate that TRAF interacting protein TRIP negatively regulates the TNFR-associated factor 2 (TRAF2) ubiquitin-dependent pathway by modulating the TRAF2-sphingosine 1-phosphate (S1P) interaction. PMID: 25716317
11. observed enhanced ubiquitylation of Poleta by TRIP E3 ligase and show that TRIP promotes Poleta localization to nuclear foci PMID: 24553286
12. The TRAF-interacting protein (TRIP) is a regulator of keratinocyte proliferation. PMID: 21068752
13. CYLD interacts with TRIP and regulates negatively nuclear factor kappaB activation by tumor necrosis factor. PMID: 14676304
14. The overexpression of TRIP sensitizes cells to TNF-induced apoptosis, an effect that can be reversed by the coexpression of Syk. PMID: 19151749
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相關(guān)疾病：

Seckel syndrome 9 (SCKL9)
亞細(xì)胞定位：

Nucleus, nucleoplasm. Nucleus, nucleolus. Chromosome. Cytoplasm. Cytoplasm, perinuclear region.
數(shù)據(jù)庫鏈接：

HGNC: 30764

OMIM: 605958

KEGG: hsa:10293

STRING: 9606.ENSP00000328203

UniGene: Hs.517972