Recombinant Human Solute carrier family 12 member 5 (SLC12A5), partial

中文名稱：

人SLC12A5重組蛋白
貨號：

CSB-YP863965HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人SLC12A5重組蛋白
貨號：

CSB-EP863965HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人SLC12A5重組蛋白
貨號：

CSB-EP863965HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SLC12A5重組蛋白
貨號：

CSB-BP863965HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人SLC12A5重組蛋白
貨號：

CSB-MP863965HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SLC12A5
Uniprot No.：

Q9H2X9
別名：

Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; Potassium Chloride Cotransporter; Potassium chloride transporter 5; rKCC2; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter) member 5; Solute carrier family 12 member 5
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.
基因功能參考文獻：
1. the long-time considered "neuron-specific" KCC2 co-transporter is expressed in pancreatic islet beta-cells where it modulates Ca(2+)-dependent insulin secretion. PMID: 28496181
2. These data clearly demonstrated that partially disrupted neuronal Cl(-) extrusion, mediated by two types of differentially impaired KCC2 mutant in an individual, causes epilepsy of infancy with migrating focal seizures. PMID: 27436767
3. Study describes the developmental patterns of cation-chloride cotransporters in the human brain from the fetal stage to senescence. Expression of KCC2 and its functionally associated proteins begins in early fetal period. PMID: 26428952
4. The authors show that APP can physically interact with KCC2, a neuron-specific K(+)-Cl(-) cotransporter that is essential for Cl(-) homeostasis and fast GABAergic inhibition. PMID: 28054918
5. SLC12A5 promoted the migration and invasion of BUC by enhancing MMP-7 expression. PMID: 28333147
6. Study shows that the overall expression of potassium-chloride cotransporter-2 is increased in the hippocampi of temporal lobe epilepsy patients. PMID: 26427846
7. Our network model suggested the loss of KCC2 in a critical number of pyramidal cells increased external potassium and intracellular chloride concentrations leading to seizure-like field potential oscillations. These oscillations included transient discharges leading to ictal-like field events with frequency spectra as in vitro Restoration of KCC2 function suppressed seizure activity and thus may present a useful therapeut PMID: 27852771
8. The KCC2 exerts specific functions for the maturation of glycinergic synapses in cultured spinal cord neurons. PMID: 26780567
9. SLC12A5 plays a pivotal oncogenic role in colorectal carcinogenesis; its overexpression is an independent prognostic factor of patients with CRC. PMID: 25947013
10. the functional deficit of KCC2 may offer an explanation for the delayed onset of Rett symptoms. PMID: 26733678
11. A KCC2 mutation causes epilepsy of infancy with migrating focal seizures. Decreased KCC2 expression, reduced protein glycosylation and impaired Cl- extrusion contribute to loss of KCC2 activity, impairing synaptic inhibition and promoting excitability. PMID: 26333769
12. these data provide insight into the mechanism regulating Cl(-) homeostasis in immature neurons, and suggest that WNK1-regulated changes in KCC2 phosphorylation contribute to the developmental excitatory-to-inhibitory GABA sequence. PMID: 26126716
13. a novel signaling pathway that couples KCC2 to the cytoskeleton and regulates the formation of glutamatergic synapses. PMID: 26056138
14. These data describe a novel KCC2 variant significantly associated with a human disease and suggest genetically encoded impairment of KCC2 functional regulation may be a risk factor for the development of human IGE. PMID: 24928908
15. KCC2-R952H from patients with febrile seizures impairs neuronal Cl- extrusion and dendritic spine formation. PMID: 24668262
16. Functional characterization of mutant SLC12A5 revealed its potential oncogenic effect in colon cancer. PMID: 24699064
17. Reduced KCC2/NKCC1 ratio in the cerebrospinal fluid of Rett Syndrome patients suggests a disturbed process of GABAergic neuronal maturation and open up a new therapeutic perspective. PMID: 23894354
18. Disrupted KCC2 activity seems to be involved in neuropathic pain, epilepsy, motor spasticity, stress and schizophrenia. PMID: 23621303
19. BPA can disrupt Kcc2 gene expression through epigenetic mechanisms PMID: 23440186
20. Alternate transcripts from KCC2 may participate in the abnormal GABA signaling in the dorsolateral prefrontal cortex associated with schizophrenia PMID: 22496567
21. Hyperpolarizing GABAergic transmission depends on KCC2 function and membrane potential. PMID: 22082832
22. KCC2 is widely expressed in several human cancer cell lines and serves to increase cervical tumourigenesis via an ion transport-independent mechanism PMID: 21911617
23. The Wnk3 protein isoforms have a similar effect on SLC12 cotransporters. NKCC1/2 and NCC were inhibited, even in hypertonicity, while KCCs were activated, even in isotonic conditions. PMID: 21613606
24. levels of KCC2 mRNA and protein were significantly decreased in the neurons around large abnormal neurons in focal cortical dysplasia PMID: 21256718
25. analysis of differences in large extracellular loop between the K(+)-Cl(-) cotransporters KCC2 and KCC4 PMID: 20516068
26. chromosome 20 mapping PMID: 11701957
27. Expression of hKCC2 cRNA in Xenopus laevis oocytes results in significant Cl(-)-dependent (86)Rb(+) uptake under isotonic conditions PMID: 12106695
28. human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl- channels by KCl through an increase in the intracellular ions, water influx and cell swelling. PMID: 12637262
29. GABAergic transmission is influenced by the neuronal expression chloride-extruding K(+)-Cl(-) cotransporter KCC2--REVIEW PMID: 15528236
30. KCC2 expression is not only necessary but is also sufficient for ending the depolarizing period of GABA in developing cortical neurons PMID: 15932617
31. constitutive K(+)-Cl(-) cotransport mediated by KCC2 is completely independent of serine-threonine phosphatase activity PMID: 16291749
32. decrease in the spontaneous activity transients was associated with a developmental up-regulation of the neuronal chloride extruder K+-Cl- cotransporter 2 PMID: 16324114
33. PKC-dependent phosphorylation of KCC2 may play a central role in modulating both the functional expression of this critical transporter in the brain and the strength of synaptic inhibition PMID: 17693402
34. The novel KCC2a isoform differs from the only previously known KCC2 isoform (now termed KCC2b) by 40 unique N-terminal amino acid residues, including a putative Ste20-related proline alanine-rich kinase-binding site. PMID: 17715129
35. the two isoforms can be coimmunoprecipitated from the neonatal brain, suggesting the presence of endogenous KCC2a-KCC2b heteromers. PMID: 19307176
36. membrane rafts render KCC2 inactive and NKCC1 active PMID: 19686239
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相關疾病：

Epileptic encephalopathy, early infantile, 34 (EIEE34); Epilepsy, idiopathic generalized 14 (EIG14)
亞細胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

SLC12A transporter family
組織特異性：

Brain specific. Detected in neuronal cells.
數據庫鏈接：

HGNC: 13818

OMIM: 606726

KEGG: hsa:57468

STRING: 9606.ENSP00000387694

UniGene: Hs.21413