Recombinant Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A)

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中文名稱：

Recombinant Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A)
品名簡稱：

Recombinant Human PPP2R1A protein
貨號：

CSB-EP018562HUa0
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

PPP2R1A
Uniprot No.：

P30153
別名：

Medium?tumor?antigen-associated?61?kDa?protein;PP2A?subunit?A?isoform?PR65-alpha;PP2A?subunit?A?isoform?R1-alpha
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
分子量：

72.1 kDa
表達區(qū)域：

2-589aa
氨基酸序列

AAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.
基因功能參考文獻：
1. PP2A controls mitotic exit through EG5 dephosphorylation. PMID: 28487562
2. Total PP2A activity and PPP2R1A-associated PP2Ac activity were significantly increased in cells overexpressing PPP2R1A-WT. In addition, overexpression of PPP2R1A-WT increased cell proliferation in vitro and tumor growth in vivo PMID: 27272709
3. PPP2R1A mutations occur in a subset of gastrointestinal stromal tumors and are associated with a high malignant potential that leads to decreased disease-free survival and overall survival PMID: 27469332
4. Results demonstrated that the promotive effect of eEF-2K on glycolysis resulted from the kinase-mediated restriction of synthesis of the protein phosphatase 2A-A (PP2A-A). PMID: 27181208
5. PPP2R1A mutation is associated with endometrial carcinoma progression and abdominopelvic metastasis. PMID: 27348297
6. PPP2R1A mutations affect PP2A function and oncogenic signaling, illuminating the genetic basis for serous EC development. PMID: 27485451
7. PR65A phosphorylation regulates PP2A complex signaling. PMID: 24465463
8. Our results suggest that IH-induced ROS generation increases PP2A activation and subsequently downregulates ERK1/2 activation, which results in inhibition of PC12 cell proliferation through G0/G1 phase arrest and NGF-induced neuronal differentiation. PMID: 24885237
9. The two functional variants in PPP2R1A and PPP2R5E and their combinations are associated with lung cancer risk in the Chinese. PMID: 24204789
10. Partial unfolding of PR65/A impacts catalysis by altering the proximity of bound catalytic subunit and substrate. PMID: 24120762
11. For PPP2R1A, a heterozygous, somatic mutation (c.771G>T, p.W257C) was identified in 1 out of 37 patients (2.7%) with primary ovarian endometrioid carcinoma. PMID: 23588898
12. gene transcription of PPP2R1A regulated by the polymorphism and methylation in the promoter region PMID: 23555712
13. This study indicates that the PPP2R1A mutation occurs at a lower frequency compared to other gynecological malignancies, irrespective of the histological subtype PMID: 23267135
14. the frequent mutation of PPP2R1A in the serous type of uterine cancer, a low frequency of mutation in endometrioid endometrial cancer and absence of mutation in uterine carcinosarcoma. PMID: 21882256
15. Our findings suggest that functional genetic variants in the proximal promoter of the PP2A-Aalpha gene and their haplotypes are critical in the regulation of transcriptional activation. PMID: 21889517
16. identified somatic missense mutations in 40.8% of high-grade serous endometrial tumours and 5% of endometrial endometrioid carcinomas; mutations identified in ovarian tumours at lower frequencies;no mutations found in high- or low-grade serous carcinoma PMID: 21381030
17. PPP2R1A somatic mutations occur in certain types of uterine and ovarian neoplastic lesions, especially uterine serous carcinomas PMID: 21435433
18. PP2A-mediated dephosphorylation of Carma1 is a critical step to limit T-cell activation and effector cytokine production. PMID: 21157432
19. genes mutated in ovarian clear cell carcinoma(OCCC);data suggest PPP2R1A functions as an oncogene and ARID1A as tumor-suppressor gene; in 42 OCCCs, 7% had mutations in PPP2R1A and 57% in ARID1A; suggests aberrant chromatin remodeling contributes to OCCC PMID: 20826764
20. Data show that upon hypoxia, the TGF-beta-induced phosphorylation of Smad3 was inhibited, although Smad2 remained phosphorylated, and Smad3 was dephosphorylated by PP2A. PMID: 19951945
21. PP2A-A alpha transcriptional regulation is mediated by multiple factors including AP-2alpha, CREB, ETS-1, and SP-1 PMID: 19750005
22. Data show that DSB promote PP2A to associate with Ku 70 and Ku 86. PMID: 19794960
23. PP2A-A has a role in the failure of beta1 integrin dephosphorylation at threonines 788 and 789 in tumor cell lines PMID: 14532964
24. PP2A-mediated dephosphorylation of BCL-2 is required to protect BCL-2 from proteasome-dependent degradation, affecting resistance to ER stress PMID: 16717086
25. dysfunction of E-cadherin due to its endocytosis may occur in some proportion of human breast carcinomas in which the PP2A-A protein is lost or significantly reduced PMID: 16930554
26. protein phosphatase 2a recruitment to I-kappaB kinase gamma/NF-kappaB essential modulator is regulated by heptad repeats, which are targeted by HTLV-I tax PMID: 17314097
27. The results of these experiments indicate that the HSF2 region comprising amino acids 343-363 is important for A subunit interaction. PMID: 17688198
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相關疾病：

Mental retardation, autosomal dominant 36 (MRD36)
亞細胞定位：

Cytoplasm. Nucleus. Chromosome, centromere. Lateral cell membrane. Cell projection, dendrite.
蛋白家族：

Phosphatase 2A regulatory subunit A family
數據庫鏈接：

HGNC: 9302

OMIM: 605983

KEGG: hsa:5518

STRING: 9606.ENSP00000324804

UniGene: Hs.467192