Recombinant Human Serine/threonine-protein kinase Nek9 (NEK9), partial

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中文名稱：

Recombinant Human Serine/threonine-protein kinase Nek9 (NEK9), partial
品名簡稱：

Recombinant Human NEK9 protein, partial
貨號(hào)：

CSB-EP837435HU
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

NEK9
Uniprot No.：

Q8TD19
別名：

Nercc1 kinase;Never in mitosis A-related kinase 9;NimA-related protein kinase 9;NimA-related kinase 8;Nek8
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

36.5 kDa
表達(dá)區(qū)域：

52-308aa
氨基酸序列

YIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQMVHSCLDQDPEQRPTADELLDRPLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
基因功能參考文獻(xiàn)：
1. High expression level of NEK9 is associated with recurrence in glioblastoma. PMID: 26956052
2. Signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14 to properly coordinate cytokinesis. PMID: 28630147
3. Recessive NEK9 mutation is associated with lethal skeletal dysplasia. PMID: 26908619
4. Somatic Mutations in NEK9 Cause Nevus Comedonicus. PMID: 27153399
5. Overall, these results highlight the complexity of virus-host interactions and identify a new role for the cellular protein Nek9 during human adenovirus infection, suggesting a role for Nek9 in regulating p53 target gene expression. PMID: 26676776
6. The C-terminal domain of Nek9 activates Nek7 through promoting back-to-back dimerization. PMID: 26522158
7. The findings demonstrate that a novel NEK9 network regulates the growth of cancer cells lacking functional p53. PMID: 25131192
8. NEK9 inhibition represents a novel anti-cancer strategy by induction of mitotic catastrophe via impairment of spindle dynamics, cytokinesis and mitotic checkpoint control. PMID: 23665325
9. Structural analysis of LC8 with both Nek9 peptides, together with different biophysical experiments, explains the observed diminished binding affinity of Nek9 to LC8 upon phosphorylation on Ser(944) within the Nek9 sequence PMID: 23482567
10. Nek9 phosphorylates NEDD1 on Ser377 driving its recruitment and thereby that of gamma-tubulin to the centrosome in mitotic cells. PMID: 22818914
11. The interaction between the human NimA-like protein kinase Nek9 and the Helicobacter HcpC has been validated by ELISA and surface plasmon resonance. PMID: 21735226
12. Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5. PMID: 21642957
13. DYNLL/LC8 protein controls signal transduction through the Nek9/Nek6 signaling module by regulating Nek6 binding to Nek9. PMID: 21454704
14. binds the Ran GTPase and regulates mitotic progression PMID: 12101123
15. Activated in mitosis, and activates nek6 and nek7 kinase. PMID: 12840024
16. mediates certain cellular processes, which are ultimately essential for interphase progression PMID: 14660563
17. The disruption of a nuclear function of NEK9 by adenovirus E1A-associated cellular proteins is reported. PMID: 17443675
18. The activity of Nek6 and Nek7, but not the tyrosine mutant, is increased by interaction with the Nek9 noncatalytic C-terminal domain, suggesting a mechanism in which the tyrosine is released from its autoinhibitory position. PMID: 19941817
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相關(guān)疾病：

Lethal congenital contracture syndrome 10 (LCCS10); Nevus comedonicus (NC); Arthrogryposis, Perthes disease, and upward gaze palsy (APUG)
亞細(xì)胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
組織特異性：

Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
數(shù)據(jù)庫鏈接：

HGNC: 18591

OMIM: 609798

KEGG: hsa:91754

STRING: 9606.ENSP00000238616

UniGene: Hs.624721