Recombinant Human Ribonuclease inhibitor (RNH1)

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中文名稱：

人RNH1重組蛋白
品名簡稱：

Recombinant Human RNH1 protein
貨號：

CSB-EP019901HUb1
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

RNH1
Uniprot No.：

P13489
別名：

Placental ribonuclease inhibitor;Placental RNase inhibitor;Ribonuclease/angiogenin inhibitor 1;RAI
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

57.3 kDa
表達區域：

2-461aa
氨基酸序列

SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGDVGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDPQCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCGVTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVLRAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISNNRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVESVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.
基因功能參考文獻：
1. Results show that a low expression of RNH1 in metastasis of bladder cancer and suggest that it might regulate the function of ILK through mutual binding. PMID: 27576342
2. a novel mechanism of ANG in regulating PI3K/AKT/mTOR signaling pathway via RI, is reported. PMID: 25193113
3. RNH1 bound to RNase 7 and suppressed its antimicrobial activity by blocking its ability to bind the cell wall of uropathogenic bacteria. PMID: 24107847
4. angiogenin and ILK signaling pathway plays a pivotal role in mediating the inhibitory effects of RI on melanoma cells growth PMID: 24769129
5. RI could play a novel role in inhibiting metastasis of bladder cancer through regulating EMT and ILK signaling pathways. PMID: 24768914
6. RNH1 as a regulator of HDACi resistance in gastric cancer PMID: 23584480
7. RI might play a novel role in the development of bladder cancer through regulating EMT and the ILK signaling pathway. PMID: 23703635
8. the anti-tumor effect of RNH1 is also involved in suppressing growth and metastasis PMID: 21125316
9. RNH1 (as a recombinant fusion protein) is localized to mitochondria, nuclei, and cytosol in HeLa and HCT cells; RNH1 appears to bind to various mitochondrial proteins. PMID: 21276451
10. Human ribonuclease inhibitor gene has antiangiogenic and antitumor effects when expressed in hematopoietic cells in mouse melanoma models. PMID: 15592448
11. The conformational and oxidative stabilities of both RIs increase upon complex formation with ribonucleases. Thus, RI has evolved to maintain its inhibition of invading ribonucleases, even when confronted with extreme environmental stress. PMID: 17956129
12. This the first measurement of the inhibition of the ribonucleolytic activity of onconase by ribonuclease inhibitor protein. PMID: 18930025
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亞細胞定位：

Cytoplasm.
數據庫鏈接：

HGNC: 10074

OMIM: 173320

KEGG: hsa:6050

STRING: 9606.ENSP00000346402

UniGene: Hs.530687