Recombinant Human RING-box protein 2 (RNF7)

中文名稱：

人RNF7重組蛋白
貨號：

CSB-BP019897HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人RNF7重組蛋白
貨號：

CSB-EP019897HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人RNF7重組蛋白
貨號：

CSB-MP019897HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

RNF7
Uniprot No.：

Q9UBF6
別名：

CKBBP 1; CKBBP1; CKII beta binding protein 1; CKII beta-binding protein 1; Rbx 2; Rbx2; RBX2_HUMAN; Regulator of cullins 2; RING box protein 2; RING finger protein 7; RING-box protein 2; RNF 7; RNF7; ROC 2; ROC2; SAG; Sensitive to apoptosis gene; Sensitive to apoptosis gene protein; Zinc RING finger protein SAG
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-113aa
氨基酸序列

ADVEDGEETCALASHSGSSGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
基因功能參考文獻：
1. We demonstrated that RNF7 knockdown induced growth suppression of prostate cancer cells and inactivated ERK1/2 pathway, which suggested RNF7 might be a potential novel therapeutic target for CRPC. PMID: 28252001
2. Results identified RNF7 to interact with CARMA2 regulating its NF-kappaB-activating capacity. Mechanistically, RNF7 influences CARMA2 signaling by regulating the ubiquitination state of MALT1 and the NF-kappaB-regulatory molecule NEMO. PMID: 29194363
3. Sag is a Kras-cooperating oncogene that promotes lung tumorigenesis PMID: 24430184
4. NEDD4-1 overexpression sensitizes cancer cells to etoposide-induced apoptosis by reducing SAG levels through targeted degradation. SAG is added to a growing list of NEDD4-1 substrates and mediates its biological function. PMID: 25216516
5. RNF7 gene variant is associated with the risk of developing liver fibrosis and cirrhosis in an Eastern European population. PMID: 28338112
6. MAF1, RNF7 and SETD3 are identified as PCNA-associated proteins in human cells and given this interaction with PCNA, Maf1, RNF7, and SetD3 are potentially involved in DNA replication, DNA repair, or associated processes. PMID: 26030842
7. These findings indicate that Rbx1 and Rbx2 can both activate Cul5-Vif E3 ligase in vitro, but they may undergo a more delicate selection mechanism in vivo. PMID: 25912140
8. Single nucleotide polymorphism rs16851720 was associated with liver fibrosis progression. PMID: 22841784
9. Data suggest that the sensitive-to-apoptosis gene may be a candidate gene for good prognosis in rectal cancer, independent of therapeutic response of different individuals. PMID: 22171132
10. SAG plays an important role in regulating ionizing radiation-induced apoptosis PMID: 20933570
11. The findings showed that SAG E3 ubiquitin ligase plays an essential role in cancer cell proliferation and tumor growth PMID: 20103673
12. SAG possesses a potent peroxidase property to decompose hydrogen peroxide in the presence of dithiothreitol PMID: 11999705
13. results show that the Ring-H2 finger motif of CKBBP1 is necessary for efficient binding to CKIIbeta, as well as for optimal cell proliferation PMID: 12470599
14. sensitive to apoptosis gene protein inhibits peroxynitrite-induced DNA damage. PMID: 12565832
15. results indicate that protein kinase CKII may control IkappaBalpha and p27Kip1 degradation and thereby G1/S phase transition through the phosphorylation of threonine 10 within CKBBP1 protein PMID: 12748192
16. These studies suggested that CK2 might regulate SAG-SCF E3 ligase activity through modulating SAG's stability, rather than its enzymatic activity directly. PMID: 16874460
17. Endogenous levels of pro-caspase 3 were decreased by overexpression of SAG protein. PMID: 17217622
18. Promotes hypoxia-inducible factor 1 alpha subunit (HIF-1 alpha) ubiquitination and degradation. PMID: 17828303
19. Sensitive to apoptosis gene may play an important role in regulating the apoptosis induced by heat shock presumably through maintaining the cellular redox status. PMID: 18454945
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

RING-box family
組織特異性：

Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
數據庫鏈接：

HGNC: 10070

OMIM: 603863

KEGG: hsa:9616

STRING: 9606.ENSP00000273480

UniGene: Hs.134623