Recombinant Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial (PDK2), partial

中文名稱：

人PDK2重組蛋白
貨號：

CSB-YP618976HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人PDK2重組蛋白
貨號：

CSB-EP618976HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人PDK2重組蛋白
貨號：

CSB-EP618976HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人PDK2重組蛋白
貨號：

CSB-BP618976HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人PDK2重組蛋白
貨號：

CSB-MP618976HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

PDK2
Uniprot No.：

Q15119
別名：

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; PDHK2; PDK2; PDK2_HUMAN; Pyruvate dehydrogenase kinase isoform 2; Pyruvate dehydrogenase kinase, isozyme 2; Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
表達區域：

-
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
基因功能參考文獻：
1. PDK2/PARL senses defects in mitochondrial bioenergetics. PMID: 28178523
2. The data demonstrate potential roles of PDK2 and ABCG2 polymorphisms in the metabolic phenotypes of Tibetan gout patients. PMID: 26909964
3. High glycolysis and PDK2 overexpression are closely linked to cisplatin resistance in head and neck cancer cells, which can be reversed by PDK2 nhibition. PMID: 26607904
4. The findings of the present study reveal a novel survival pathway that functionally couples the unique glycolytic phenotype in cancer cells to hypoxia resistance via a PDK2-dependent mechanism that switches Bnip3 from cell death to survival. PMID: 26416963
5. The final compound of this series, 2-[(2,4-dihydroxyphenyl)sulfonyl]isoindoline-4,6-diol, designated PS10, inhibits all four PDK isoforms with IC50 = 0.8 muM for PDK2. PMID: 24356970
6. Germline mutations in PKD2 gene is associated with autosomal-dominant polycystic kidney disease. PMID: 23300259
7. we for the first time demonstrated that a low-nutrient condition drives cancer cells to utilize glycolysis to produce ATP, and this increases the Warburg effect through a novel mechanism involving ROS/AMPK-dependent activation of PDK. PMID: 23376776
8. Mitochondrial activation by inhibition of PDKII suppresses HIF1a signaling and angiogenesis in cancer. PMID: 22614004
9. Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species. PMID: 22910903
10. Results established that wild-type p53 prevents manifestation of the Warburg effect by controlling Pdk2. These findings elucidate a new mechanism by which p53 suppresses tumorigenesis acting at the level of cancer cell metabolism. PMID: 22123926
11. PKD2 mutations are associated with autosomal dominant polycystic kidney disease. PMID: 21115670
12. study of facilitated interaction between the pyruvate dehydrogenase kinase isoform 2 and the dihydrolipoyl acetyltransferase PMID: 12816949
13. The increased PDK activity was independent of changes in intra-mitochondrial effectors, and PDK-2 and PDK-4 protein content, suggesting that it was caused by a change in the specific activity of the existing kinases. PMID: 15169745
14. A mechanism for pyruvate dehydrogenase kinase isoform 2 regulation is supported in which kinase activity is limited by ADP dissociation and pyruvate binding. PMID: 15491150
15. Reductive acetylation stimulates activity of pyruvate dehydrogenase kinase isoform 2 by speeding up ADP dissociation. PMID: 15491151
16. in human muscle, hormonal and nutritional conditions may control PDK2 and PDK4 mRNA expression via a common signalling mechanism. PMID: 15955060
17. Crystallographic studies reveal several PDHK2 structures with C-terminal cross arms that span a large trough region between the N-terminal regulatory (R) domains of the PDHK2 dimers. Three novel ligand binding sites are located in the R domain of PDHK2. PMID: 16401071
18. analysis of ligand induced effects on pyruvate dehydrogenase kinase isoform 2 PMID: 16517984
19. These studies identify ORP9 as a PDK-2 substrate and negative regulator of Akt phosphorylation at the PDK-2 site. PMID: 16962287
20. PDK2, PDK3 and PDK4 are primary PPARbeta/delta target genes in humans underlining the importance of the receptor in the control of metabolism PMID: 17669420
21. Critical role of specific ions for ligand-induced changes regulating pyruvate dehydrogenase kinase isoform 2. PMID: 18220414
22. Pi is suggested to facilitate transmission within PDHK2 of the stimulatory signal of acetylation from the distal lipoyl-group binding site to the active site. PMID: 18220415
23. mitochondrial ND2 mutation contributes to HIF1alpha accumulation via increased ROS production, up-regulation of PDK2, attenuating PDH activity, thereby increasing pyruvate, resulting in HIF1alpha stabilization PMID: 19147752
24. PKD1 children have more and larger renal cysts, larger kidneys and higher ambulatory BP than do PKD2 children. PMID: 19194729
25. the DW-motif has a pivotal role in mediating communications between the DCA-, the nucleotide-, and the lipoyl domain-binding sites of PDK2 PMID: 19833728
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亞細胞定位：

Mitochondrion matrix.
蛋白家族：

PDK/BCKDK protein kinase family
組織特異性：

Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
數據庫鏈接：

HGNC: 8810

OMIM: 602525

KEGG: hsa:5164

STRING: 9606.ENSP00000420927

UniGene: Hs.256667