Recombinant Human Proto-oncogene DBL (MCF2), partial

中文名稱：

人MCF2重組蛋白
貨號：

CSB-YP013579HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人MCF2重組蛋白
貨號：

CSB-EP013579HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人MCF2重組蛋白
貨號：

CSB-EP013579HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人MCF2重組蛋白
貨號：

CSB-BP013579HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人MCF2重組蛋白
貨號：

CSB-MP013579HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MCF2
Uniprot No.：

P10911
別名：

MCF2; DBLProto-oncogene DBL; Proto-oncogene MCF-2) [Cleaved into: MCF2-transforming protein; DBL-transforming protein]
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
基因功能參考文獻：
1. Results support a role for Dbl oncogene in mammary epithelial cell differentiation and transformation and suggest the relevance of GEF deregulation in tumor onset and progression. PMID: 25723869
2. Phosphoinositide 3-kinase C2beta regulates RhoA and the actin cytoskeleton through an interaction with Dbl PMID: 22984590
3. Dbl is regulated by hamartin through association with ezrin. PMID: 21712385
4. proto-oncogene dbl is not a major target for sporadic testicular germ cell tumors PMID: 19373475
5. data describe 4 splicing variants of the human DBL (MCF-2) proto-oncogene that show different tissue distributions and guanine nucleotide exchange factor specificities PMID: 12445822
6. provides fundamental data on the structure of Mcf-2, which regulates a variety of cellular signaling pathways PMID: 13679059
7. These data suggest a mechanism whereby precise spatial guanine nucleotide exchange of Cdc42 by Dbl is dependent on functional ERM proteins and is important for directional cell migration. PMID: 17538024
8. Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain PMID: 17606614
9. Nm23-H1 can negatively regulate cell migration and tumor metastasis by modulating the activity of Cdc42 and possibly other Rho family members through interaction with Dbl-1 PMID: 18728402
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相關疾病：

MCF2 and DBL represent two activated versions of the same proto-oncogene.
亞細胞定位：

Cytoplasm.; [Isoform 1]: Membrane.; [Isoform 3]: Membrane. Note=Colocalizes with CDC42 to plasma membrane.
蛋白家族：

MCF2 family
組織特異性：

Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, int
數據庫鏈接：

HGNC: 6940

OMIM: 311030

KEGG: hsa:4168

STRING: 9606.ENSP00000430276

UniGene: Hs.387262