Recombinant Human Protein Spindly (CCDC99)

中文名稱：

人SPDL1重組蛋白
貨號：

CSB-YP842662HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人SPDL1重組蛋白
貨號：

CSB-EP842662HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人SPDL1重組蛋白
貨號：

CSB-EP842662HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SPDL1重組蛋白
貨號：

CSB-BP842662HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人SPDL1重組蛋白
貨號：

CSB-MP842662HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SPDL1
Uniprot No.：

Q96EA4
別名：

AA409762; Arsenite related gene 1 protein; Arsenite-related gene 1 protein; CCDC 99; Ccdc99; Coiled coil domain containing 99; Coiled-coil domain-containing protein 99; FLJ20364; FLJ40690; hSpindly; MGC124977; Protein Spindly; rhabdomyosarcoma antigen MU RMS 40.4A; Rhabdomyosarcoma antigen MU-RMS-40.4A; Rrhabdomyosarcoma antigen protein MU RMS 40.4A; SPDL1; SPDLY_HUMAN; Spindle apparatus coiled coil domain containing protein 1; Spindle apparatus coiled coil protein 1; Spindly; Spindly homolog
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-605
氨基酸序列

MEADIITNLR CRLKEAEEER LKAAQYGLQL VESQNELQNQ LDKCRNEMMT MTESYEQEKY TLQREVELKS RMLESLSCEC EAIKQQQKMH LEKLEEQLSR SHGQEVNELK TKIEKLKVEL DEARLSEKQL KHQVDHQKEL LSCKSEELRV MSERVQESMS SEMLALQIEL TEMESMKTTL KEEVNELQYR QEQLELLITN LMRQVDRLKE EKEEREKEAV SYYNALEKAR VANQDLQVQL DQALQQALDP NSKGNSLFAE VEDRRAAMER QLISMKVKYQ SLKKQNVFNR EQMQRMKLQI ATLLQMKGSQ TEFEQQERLL AMLEQKNGEI KHLLGEIRNL EKFKNLYDSM ESKPSVDSGT LEDNTYYTDL LQMKLDNLNK EIESTKGELS IQRMKALFES QRALDIERKL FANERCLQLS ESENMKLRAK LDELKLKYEP EETVEVPVLK KRREVLPVDI TTAKDACVNN SALGGEVYRL PPQKEETQSC PNSLEDNNLQ LEKSVSIYTP VVSLSPHKNL PVDMQLKKEK KCVKLIGVPA DAEALSERSG NTPNSPRLAA ESKLQTEVKE GKETSSKLEK ETCKKLHPIL YVSSKSTPET QCPQQ
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Plays a role in cell migration.
基因功能參考文獻：
1. Farnesylation of Spindly is essential for its localization, & thus for proper localization of dynein & dynactin, to prometaphase kinetochores. Both Spindly and CENP-E farnesylation are required for efficient chromosome congression PMID: 25808490
2. hSpindly is farnesylated in vivo and farnesylation is essential for its interaction with the RZZ complex and hence Kinetochore localization. PMID: 25825516
3. Spindly is not only required for kinetochore localization of dynein but is a functional component of a mechanism that couples dynein-dependent poleward movement of chromosomes to their efficient attachment to microtubules. PMID: 20427577
4. dynein-mediated removal of Spindly from microtubule-attached kinetochores, rather than poleward transport per se, is the critical reaction in checkpoint silencing PMID: 20439434
5. [hSpindly] Localization of human Spindly (hSpindly) to kinetochores is controlled by the Rod/Zw10/Zwilch (RZZ) complex and Aurora B. PMID: 19468067
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
蛋白家族：

Spindly family
數據庫鏈接：

HGNC: 26010

OMIM: 616401

KEGG: hsa:54908

STRING: 9606.ENSP00000265295

UniGene: Hs.368710