Recombinant Human Protein AF-9 (MLLT3), partial

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中文名稱：

Recombinant Human Protein AF-9 (MLLT3), partial
品名簡稱：

Recombinant Human MLLT3 protein, partial
貨號：

CSB-EP014635HU1
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

MLLT3
Uniprot No.：

P42568
別名：

ALL1-fused gene from chromosome 9 protein;Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein;YEATS domain-containing protein 3
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

23.2 kDa
表達區域：

1-138aa
氨基酸序列

MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me). Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than confering, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L.
基因功能參考文獻：
1. miR-564 inhibited metastasis and proliferation of prostate cancer by targeting MLLT3 PMID: 29164580
2. MLL-AF9 fusion is associated with acute myeloid leukemia. PMID: 28114278
3. Structural insights into H3 histone crotonyl-lysine recognition by the AF9 YEATS domain have been presented. PMID: 27545619
4. A luciferase reporter gene assay revealed that hsp70 promoter activation is enhanced by the transcriptional co-activator AF9 and splicing mediator SNRPE, but suppressed by the coiled-coil domain-containing protein CCDC127. PMID: 26873636
5. YEATS domain of AF9 directly links histone crotonylation to active transcription. PMID: 27105114
6. Exploring the mechanism how AF9 recognizes and binds H3K9ac by molecular dynamics simulations and free energy calculations. PMID: 27312527
7. Studies identified the evolutionarily conserved Af9 YEATS domain as a novel acetyllysine-binding module and established a direct link between histone acetylation and DOT1L-mediated H3K79 methylation in transcription control. PMID: 25417107
8. Interaction with CBX8 precludes AF9-DOT1L binding. PMID: 23891621
9. We demonstrate that leukemogenic activity of MLL-AF9 requires RUVBL2 (RuvB-like 2), an AAA+ ATPase family member that functions in a wide range of cellular processes, including chromatin remodeling and transcriptional regulation. PMID: 23403462
10. AF9 functions as a signaling hub that regulates transcription through dynamic recruitment of cofactors in normal hematopoiesis and in acute leukemia. PMID: 23260655
11. we identified a novel fusion gene consisted of PAX5 and MLLT3 during progression from chronic phase to blastic phase in chronic myeloid leukemia PMID: 22309891
12. MLL-AF9-negative U937 cells but not positive MLL-AF9 THP-1 cells, suggesting that the toxocity ting was not antibody-mediated by antibodies against MLL-AF9 protein. PMID: 21959947
13. our data suggest: (i) C14ORF28, GNB2L1, MLLT3, DRD2 and DARPP-32 are important in the pathogenesis of schizophrenia and bipolar disorder PMID: 20874815
14. The patients with immunophenotype of Pre-B-acute lymphoblastic leukemia were found to carry: MLL/AF9. PMID: 16215946
15. Down-regulation of AF9 is not necessary to abolish malignant phenotypes by induction of terminal monocyte-macrophage differentiation in leukaemic cells carrying t(9;11)(p22;q23). PMID: 16328057
16. MLLT3 as a regulator of early erythroid and megakaryocytic cell fate in the human system. PMID: 18371451
17. Diversin, containing several nuclear localization signals, translocates to the nucleus, where it interacts with the transcription factor AF9. PMID: 19591803
18. AF17 competes with AF9 to bind Dot1a, decreases Dot1a nuclear expression by possibly facilitating its nuclear export, and relieves Dot1a.AF9-mediated repression of alpha-ENaC and other target genes. PMID: 19864429
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相關疾病：

A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with KMT2A/MLL1. The result is a rogue activator protein.
亞細胞定位：

Nucleus. Chromosome.
組織特異性：

Enriched in undifferentiated hematopoietic stem cells in fetal liver, cord blood and bone marrow.
數據庫鏈接：

HGNC: 7136

OMIM: 159558

KEGG: hsa:4300

STRING: 9606.ENSP00000369695

UniGene: Hs.317248