Recombinant Human Pre-mRNA-processing factor 19 (PRPF19)

中文名稱：

人PRPF19重組蛋白
貨號：

CSB-YP891786HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人PRPF19重組蛋白
貨號：

CSB-EP891786HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人PRPF19重組蛋白
貨號：

CSB-EP891786HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人PRPF19重組蛋白
貨號：

CSB-BP891786HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人PRPF19重組蛋白
貨號：

CSB-MP891786HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

PRPF19
Uniprot No.：

Q9UMS4
別名：

hPso4; NMP200; Nuclear matrix protein 200; Nuclear matrix protein NMP200 related to splicing factor PRP19; pre mRNA processing factor 19; Pre-mRNA-processing factor 19; PRP19; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae); PRP19_HUMAN; PRPF19; PSO4; psoralen 4; Senescence evasion factor; SNEV; UBOX4
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-504
氨基酸序列

SLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR QELSHALYQH DAACRVIARL TKEVTAAREA LATLKPQAGL IVPQAVPSSQ PSVVGAGEPM DLGELVGMTP EIIQKLQDKA TVLTTERKKR GKTVPEELVK PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG GADKNVVVFD KSSEQILATL KGHTKKVTSV VFHPSQDLVF SASPDATIRI WSVPNASCVQ VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG CSLTCAQFHP DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF SENGYYLATA ADDSSVKLWD LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL ALGGTDVQIY ICKQWTEILH FTEHSGLTTG VAFGHHAKFI ASTGMDRSLK FYSL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.
基因功能參考文獻：
1. This work implies that the gain of Prp19 is a critical event during the progression of hepatocellular carcinoma PMID: 26959880
2. These results demonstrate that SNEV regulates adipogenesis in hASCs and indicate that DDR capacity in general might be a pre-requisite for this process. PMID: 28041875
3. the interactome of influenza A virus NS1 in host cells was comprehensively profiled, and our findings reveal a novel regulatory role for PRP19 in viral replication PMID: 27096427
4. Crystal structure revealed features of the WD40 domain of human PRPF19. PMID: 28962858
5. The results suggest that RPA phosphorylation enhances the recruitment of PRP19 to RPA-ssDNA and stimulates RPA ubiquitylation through a process requiring both PRP19 and RFWD3, thereby triggering a phosphorylation-ubiquitylation circuitry that promotes ATR activation and homologous recombination. PMID: 28666352
6. role of Pso4 in DNA repair and DNA damage PMID: 26364595
7. Study demonstrates that the levels of Prp19 and Cdc5L are overexpressed in hepatocellular carcinoma (HCC). Prp19 binds with Cdc5L and its downregulation results in reduction of Cdc5L. Mechanistic insights reveal that silencing Prp19 compromises translation activity of Cdc5L and facilitates lysosome-induced degradation of Cdc5L in HCC cells. PMID: 28387715
8. We report that PRP19 expression is elevated in lung carcinoma tissues compared to non-tumor tissues PMID: 24731397
9. These findings suggest that SKAP promotes ultraviolet rays-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19. PMID: 24718257
10. involvement of hPso4 in the recombinational repair of DSBs PMID: 24675077
11. DNA damage induces down-regulation of Prp19 via impairing Prp19 stability in hepatocellular carcinoma cells. PMID: 24587161
12. The Prp19-CDC5L complex plays a crucial role during human pre-mRNA splicing by catalytic activation of the spliceosome. PMID: 24598747
13. Prp19 complex as the first spliceosome subcomplex that directly contributes to mitosis in vertebrates independently of its function in interphase. PMID: 24069358
14. PRP19 directly binds RPA and localizes to DNA damage sites via RPA, promoting RPA ubiquitylation in a DNA-damage-induced manner. PMID: 24332808
15. New insights into pre-mRNA processing factor 19 PMID: 22967061
16. PRP19 over-expression is associated with hepatocellular carcinoma recurrence. PMID: 23220010
17. SNEV phosphorylation at S149 is essential for mediating the cytoprotective effect of SNEV upon DNA damage/oxidative stress and partially contributes to the life span extension caused by SNEV overexpression. PMID: 22529335
18. hPso4, once it forms a complex with Metnase, negatively regulates Metnase's TIR binding activity PMID: 20416268
19. Purified hPrp19/CDC5L complexes exhibit an elongated, asymmetric shape with a maximum dimension of approximately 20 nm. PMID: 20176811
20. concluded that hPso4 plays a major and previously undefined role in mammalian DNA DSB repair[Pso4] PMID: 12960389
21. required prior to catalytic step 1 of splicing, but not for stable integration of U4/U6.U5 tri-snRNPs PMID: 15175653
22. report the identification of two additional factors required in the DNA interstrand cross-links repair process, a previously characterized pre-mRNA splicing complex composed of Pso4/Prp19 and Werner syndrome protein(WRN) PMID: 16223718
23. SNEV, the human orthologue of yeast PRP19, functions in splicing and homo-oligomerization of SNEV in HeLa nuclear extract is essential for spliceosome assembly. PMID: 16332694
24. The high levels of SNEV might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells. PMID: 16388800
25. ubiquitylated hPrp19 fails to interact with either Cdc5L or Plrg1 indicating that DNA damage can induce profound alterations to hPrp19 core complex. PMID: 17276391
26. hPso4 is necessary to bring Metnase to the DSB sites for its function(s) in DNA repair PMID: 18263876
27. Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites [Blom7alpha] PMID: 19641227
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亞細胞定位：

Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Lipid droplet.
蛋白家族：

WD repeat PRP19 family
組織特異性：

Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
數據庫鏈接：

HGNC: 17896

OMIM: 608330

KEGG: hsa:27339

STRING: 9606.ENSP00000227524

UniGene: Hs.502705