Recombinant Human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PIK3C2A), partial

中文名稱：

人PIK3C2A重組蛋白
貨號(hào)：

CSB-YP017994HU
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

人PIK3C2A重組蛋白
貨號(hào)：

CSB-EP017994HU
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

人PIK3C2A重組蛋白
貨號(hào)：

CSB-EP017994HU-B
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人PIK3C2A重組蛋白
貨號(hào)：

CSB-BP017994HU
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

人PIK3C2A重組蛋白
貨號(hào)：

CSB-MP017994HU
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

PIK3C2A
Uniprot No.：

O00443
別名：

C2 containing phosphatidylinositol kinase; C2 domain containing alpha polypeptide; Class 2 alpha polypeptide; CPK; Cpk m; DKFZp686L193; MGC142218; OTTHUMP00000231763; P3C2A_HUMAN; Phosphatidylinositol 3 kinase class 2 alpha; Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; Phosphoinositide 3 kinase class 2 alpha; Phosphoinositide 3 kinase; class 2; alpha polypeptide; Phosphoinositide 3-Kinase-C2-alpha; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha; PI3KC 2 alpha; PIK3C 2A; Pik3c2a; PtdIns-3-kinase C2 subunit alpha
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components.
基因功能參考文獻(xiàn)：
1. PIK3C2A Polymorphism is associated with recurrence in non-small cell lung cancer. PMID: 28150169
2. Four gene signature (PTEN, PIK3C2A, ITPA and BCL3) is an independent prognostic factors of both overall survival and disease-free survival in clear-cell renal-cell carcinoma. PMID: 27779101
3. These results elucidated that PIK3C2A mRNA acts as a miR-124 decoy to regulate CD151 and to affect hepatocellular carcinoma malignant phenotypes. PMID: 27270320
4. PIK3C2A was associated with bipolar disorder. PMID: 28195573
5. Results present evidence that PI3K-C2alpha is critical in the proper maturation of endosomes, and for coordinating the use of endosomes as an additional membrane source during autophagy. PMID: 28910396
6. miR-518a-5p was downregulated in imatinib-resistant gastrointestinal stromal tumors (GISTs), and the expression of miR-518a-5p was confirmed with good concordance between real-time PCR and miRNA microarray results. Luciferase reporter assays indicated that miR-518a-5p bound to the PIK3C2A 3'UTR. PMID: 26950487
7. Therefore, PI3K-C2A is a factor important for human cytomegalovirus replication and has a role in the production of human cytomegalovirus virions. PMID: 27412598
8. PI3KC2a knockdown results in rerouting of insulin signaling and pancreatic beta cell proliferation. PMID: 26387957
9. MiR-206 regulates endothelial progenitor cell activities by targeting PIK3C2alpha expression.The role of PIK3C2alpha in endothelial progenitor cell function in coronary artery disease PMID: 26175229
10. PI3KC2alpha has a role in regulating shear-dependent platelet adhesion via regulation of membrane structure, rather than acute signalling PMID: 25779105
11. a model of S. flexneri dissemination is proposed in which the formation of vacuole-like protrusions is mediated by the PIK3C2A-dependent production of the signaling lipid phosphatidylinositol 3-phosphate in the protrusion membrane PMID: 25667265
12. Data (including data from knockout mice) suggest that PI3KC2a participates in tumor angiogenesis. [REVIEW] PMID: 25233418
13. These observations indicate that PI3K-C2alpha plays the pivotal role in TGFbeta receptor endocytosis and thereby Smad2/3 signaling, participating in angiogenic actions of TGFbeta. PMID: 25614622
14. Class II enzyme PI3K-C2beta and the class IB isozyme p110gamma mainly regulate the S1P- and high density lipoprotein (HDL)-dependent endothelial cell (EC) migration and PI3K-C2alpha primarily controls EC survival PMID: 23320105
15. PI3K-C2alpha plays the crucial role in S1P(1) internalization into the intracellular vesicular compartment, Rac1 activation on endosomes, and thereby migration through regulating vesicular trafficking in ECs PMID: 23192342
16. These data suggest a general role for PI3KC2alpha in regulating physiologically relevant dynamin-independent internalization pathways. PMID: 21081650
17. Phosphoinositide 3-kinase C2alpha was found to be differentially regulated by alpha(v)beta(3) and alpha(v)beta(5) integrin, and may represent a new regulatory pathway of cell migration downstream of integrin engagement. PMID: 12237112
18. Nickel compounds induce HIF-1 transactivation and Cap43 protein expression through a PI-3K/Akt-dependent and p70(S6k)- independent pathway. PMID: 14729612
19. VEGFR1 and VEGFR2 initiate a clonogenic response in myeloid leukemia cells that is PI3-kinase dependent. PMID: 15183893
20. demonstrated that the isolated clathrin binding domain of Phosphoinositide 3-kinase C2alpha can drive clathrin lattice assembly and can independently modulate clathrin distribution and function when expressed in cells PMID: 16215232
21. analysis of the PI specificities of PX domains and the mechanism by which the PI3K-C2alpha PX domain interacts with PtdIns(4,5)P(2)-containing membranes PMID: 17038310
22. molecular linkage between PI3K-C2alpha and the microtubule motor machinery, with implications for membrane trafficking in intact cells PMID: 17110375
23. phosphoinositide 3-kinase C2alpha has a role in insulin signaling PMID: 17644513
24. Lowering the levels of PI3K-C2alpha in a number of cancer cell lines reduces their proliferation and cell viability. PMID: 18403640
25. Taken together, these data suggest for the first time that in addition to class I PI3Ks in cancer, class II PIK3C2alpha can modulate hepatocellular carcinoma cell growth. PMID: 19591801
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亞細(xì)胞定位：

Cell membrane. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus. Cytoplasm. Golgi apparatus, trans-Golgi network.
蛋白家族：

PI3/PI4-kinase family
組織特異性：

Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest l
數(shù)據(jù)庫(kù)鏈接：

HGNC: 8971

OMIM: 603601

KEGG: hsa:5286

STRING: 9606.ENSP00000265970

UniGene: Hs.175343