Recombinant Human Pericentrin (PCNT), partial

中文名稱：

人PCNT重組蛋白
貨號：

CSB-YP017627HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人PCNT重組蛋白
貨號：

CSB-EP017627HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人PCNT重組蛋白
貨號：

CSB-EP017627HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人PCNT重組蛋白
貨號：

CSB-BP017627HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人PCNT重組蛋白
貨號：

CSB-MP017627HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

PCNT
Uniprot No.：

O95613
別名：

Centrosome Marker; Ken; Kendrin; KIAA0402; MOPD2; PCN; PCNT 2; PCNT; PCNT B; PCNT_HUMAN; PCNT1; PCNT2; PCNTB; PCTN2; Pericentrin 1; Pericentrin 2; Pericentrin 380; Pericentrin; Pericentrin B; Pericentrin-B; SCKL4
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
基因功能參考文獻：
1. High pericentrin expression is associated with Disruptive Cilia Formation in down syndrome. PMID: 30100262
2. we identified two novel mutations in the PCNT gene associated with Microcephalic osteodysplastic primordial dwarfism type II and intracranial aneurysms PMID: 26231886
3. PCNT has to be phosphorylated by PLK1 to be a suitable substrate of separase. PMID: 26647647
4. Cep68 degradation allows Cep215 removal from peripheral pericentriolar material (PCM) preventing centriole separation following disengagement, PCNT cleavage mediates Cep215 removal from core of the PCM to inhibit centriole disengagement and duplication PMID: 25503564
5. The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. PMID: 24466316
6. Data suggest that changes in the expression levels of PCNT in DS subjects may be involved into the molecular mechanism of Down's syndrome. PMID: 23979692
7. PCNT gene mutation is associated with hematological abnormalities in patients with microcephalic osteodysplastic primordial dwarfism type II. PMID: 24106199
8. Che-1 depletion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in its turn, deregulates the activation of centrosomal cyclin B-Cdk1 and advances entry into mitosis. PMID: 23798705
9. The pericentrin B cleavage is essential for timely centriole disengagement and duplication. PMID: 22722493
10. Kendrin is a novel and crucial substrate for separase at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis. PMID: 22542101
11. Expression of RRP1B, PCNT, KIF21A and ADRB2 in leucocytes of Down's syndrome subjects, was analyzed. PMID: 22552340
12. The pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. PMID: 22184200
13. PCNT has a crucial role in tooth development; teeth of a patient with a novel homozygous mutation p.Glu1154X are probably the smallest ever reported. PMID: 21567919
14. Severe insulin resistance and premature diabetes are common features of PCNT deficiency but are not congenital. PMID: 21270239
15. Microcephalic osteodysplastic primordial dwarfism type II is a genetically homogeneous condition due to loss-of-function of pericentrin. PMID: 19643772
16. Results found no significant association between the pericentrin gene and schizophrenia in the Japanese population. This gene may not play a major role independently in the etiology of SZ. PMID: 19937158
17. The NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.(Pericentrin) PMID: 20567258
18. Data suggest that pericentrin may regulate the intracellular distribution and secretion of insulin. PMID: 20676397
19. CG-NAP and kendrin provide sites for microtubule nucleation in the mammalian centrosome by anchoring gamma-TuRC PMID: 12221128
20. results confirm that MT1-MMP cleaves pericentrin-2 in humans but not in mice and that mouse models of cancer probably cannot be used to critically examine MT1-MMP functionality PMID: 16251193
21. Elevated levels of PCNT2 might be implicated in the pathophysiology of bipolar disorder. PMID: 17884020
22. Mutations in PCNT cause Seckel syndrome with defective ATR-dependent DNA damage signaling. PMID: 18157127
23. study found that biallelic loss-of-function mutations in the centrosomal pericentrin (PCNT) gene on chromosome 21q22.3 cause microcephalic osteodysplastic primordial dwarfism type II (MOPD II) in 25 patients PMID: 18174396
24. Upregulation of pericentrin is associated with increased angiogenesis and prostate tumor cell proliferation PMID: 18701509
25. These findings suggest that the DISC1-kendrin interaction plays a key role in the microtubule dynamics. PMID: 18955030
26. The association of PCNT2 and DBZ with schizophrenia and bipolar disorder in a case-control study of Japanese cohorts, was examined. PMID: 19191256
27. results suggest that genetic variations in the PCNT gene may play a significant role in the etiology of major depressive disorder in the Japanese population PMID: 19448849
28. Results show that a lack of microcephalin or pericentrin results in a loss of Chk1 from centrosomes with subsequently deregulated activation of centrosomal cyclin B-Cdk1. PMID: 19546241
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相關疾?。?/div>
Microcephalic osteodysplastic primordial dwarfism 2 (MOPD2)
亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
組織特異性：

Expressed in all tissues tested, including placenta, liver, kidney and thymus.
數據庫鏈接：

HGNC: 16068

OMIM: 210720

KEGG: hsa:5116

STRING: 9606.ENSP00000352572

UniGene: Hs.474069