Recombinant Human Peptidyl-tRNA hydrolase ICT1, mitochondrial (ICT1)

中文名稱：

人MRPL58重組蛋白
品名簡稱：

Recombinant Human ICT1 protein
貨號：

CSB-EP613490HU
說明書：

MSDS Datasheet
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

MRPL58
Uniprot No.：

Q14197
別名：

Digestion substraction 1; DS-1; DS1; Ict1; ICT1_HUMAN; Immature colon carcinoma transcript 1; Immature colon carcinoma transcript 1 protein; mitochondrial; Peptidyl-tRNA hydrolase ICT1; Peptidyl-tRNA hydrolase ICT1, mitochondrial
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

36.4kDa
表達區域：

30-206aa
氨基酸序列

LHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADSDIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
基因功能參考文獻：
1. High ICT1 expression is associated with leukemia. PMID: 29328466
2. ICT1 overexpression notably predicted poor prognosis of gastric cancer patients. PMID: 28987942
3. Study demonstrates that ICT1 overexpression correlates with malignant clinical features and poor prognosis of hepatocellular carcinoma (HCC) patients. Functionally, ICT1 promotes proliferation, cell cycle progression and inhibits apoptosis of HCC cells. ICT1 regulates the expressions of CDK1, cyclin B1, Bcl-2 and Bax, and is directly targeted by microRNA-134 (miR-134) in HCC cells. PMID: 29130100
4. ICT1 may play a crucial role in promoting gastric cancer proliferation in vitro. PMID: 29036264
5. The results provide direct evidence that localized feedbacks on Fat4-Ds1 complexes can give rise to planar cell polarity. PMID: 28826487
6. our preliminary study reveals the important role of ICT1 in NSCLC cells that knockdown of ICT1 significantly suppressed cell proliferation, arrested cell cycle though altering expression cell cycle regulators, and promoted cell apoptosis. PMID: 27413166
7. ICT1 knockdown significantly inhibited cell proliferation and migration, led to G2/M phase cell-cycle arrest, and triggered apoptosis via the intracellular AMPKa, SAPK/JNK, and PARP signaling pathways. PMID: 27411551
8. ICT1 was significantly upregulated in diffuse large B-cell lymphoma tissues (DLBCL) and correlated with poor survival. Knockdown of ICT1 remarkably induced cell cycle G0/G phase arrest and apoptosis in DLBCL cells. PMID: 28629825
9. ICT1 knockdown altered the expression of apoptosis- or cell cyclerelated proteins such as Bcl-2, caspase-3, CDK1, CDK2 and cyclin B. PMID: 28290601
10. ICT1 may be essential for hydrolysis of prematurely terminated peptidyl-tRNA moieties in stalled mitoribosomes. PMID: 20186120
顯示更多

收起更多
亞細胞定位：

Mitochondrion.
蛋白家族：

Prokaryotic/mitochondrial release factor family, Mitochondrion-specific ribosomal protein mL62 subfamily
組織特異性：

Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells.
數據庫鏈接：

HGNC: 5359

OMIM: 603000

KEGG: hsa:3396

STRING: 9606.ENSP00000301585

UniGene: Hs.407955