Recombinant Human PH domain leucine-rich repeat-containing protein phosphatase 1 (PHLPP1), partial

中文名稱：

人PHLPP1重組蛋白
貨號：

CSB-YP017936HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人PHLPP1重組蛋白
貨號：

CSB-EP017936HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人PHLPP1重組蛋白
貨號：

CSB-EP017936HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人PHLPP1重組蛋白
貨號：

CSB-BP017936HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人PHLPP1重組蛋白
貨號：

CSB-MP017936HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

PHLPP1
Uniprot No.：

O60346
別名：

hSCOP; KIAA0606; PH domain and leucine rich repeat protein phosphatase; PH domain leucine rich repeat containing protein phosphatase; PH domain leucine rich repeat protein phosphatase ; PH domain leucine-rich repeat-containing protein phosphatase 1; PH domain-containing family E member 1; PHLP1_HUMAN; PHLPP; Phlpp1; Pleckstrin homology domain containing family E (with leucine rich repeats) member 1; Pleckstrin homology domain containing family E protein 1; Pleckstrin homology domain-containing family E member 1; PLEKHE 1 protein; PLEKHE1; SCN circadian oscillatory protein; SCOP; Suprachiasmatic nucleus circadian oscillatory protein; Suprachiasmatic nucleus circadian protein
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts. Involved in the hippocampus-dependent long-term memory formation. Involved in circadian control by regulating the consolidation of circadian periodicity after resetting. Involved in development and function of regulatory T-cells.
基因功能參考文獻：
1. Data suggest a tumor suppressor role of Xist in inhibiting AKT activation via regulation of non-X-chromosome gene PHLPP1 expression. PMID: 27248326
2. Low PHLPP expression is associated with gallbladder cancer. PMID: 28212545
3. nickel exposure results in DNMT3b induction and MEG3 promoter hypermethylation and expression inhibition, further reduces its binding to c-Jun and in turn increasing c-Jun inhibition of PHLPP1 transcription, leading to the Akt/p70S6K/S6 axis activation, and HIF-1alpha protein translation, as well as malignant transformation of human bronchial epithelial cells. PMID: 28263966
4. Studies indicate that two PHLPP isozymes, PHLPP1 and PHLPP2, were identified in a search for phosphatases that dephosphorylate Akt, and thus suppress growth factor signaling. PMID: 27913677
5. During LPS induced macrophage inflammatory response, PHLPP levels in macrophages are depleted through the inhibition of SP1 dependent transcriptional regulation. PMID: 28322791
6. Abnormal PHLPP, FoxO3a, and RAD51 protein expressions may be involved in the development of high- and low-grade ovarian serous adenocarcinomas, suggesting common molecular pathways. PMID: 28139510
7. this study shows that PHLPP1 played a suppression role in inflammatory response of glioma PMID: 26971226
8. Increased PHLPP expression is associated with pancreatic cancer. PMID: 26760962
9. our results suggest that PHLPP1 plays a crucial role in sacral chordoma PMID: 26823799
10. high levels of PHLPP might reflect a less aggressive lung adenocarcinoma phenotype and predict better survival in patients with lung adenocarcinoma. PMID: 26463718
11. PHLPP1, along with miR-522, promoted tumor cell growth in glioblastoma cells. PMID: 25776496
12. Suppression of PHLPP1 by DNA methylation contributes to melanoma development and progression. PMID: 24121273
13. PHLPP1 is a binding protein for Mst1 and it modulates the Hippo pathway by dephosphorylating Mst1 at the inhibitory Thr(387) of Mst1. PMID: 24393845
14. our results reveal WDR48 and USP12 as novel PHLPP1 regulators and potential suppressors of tumor cell survival. PMID: 24145035
15. The overall survival time and relapse-free survival time in PHLPP1-positive gastric cancer patients were significantly longer than in PHLPP1-negative patients. PMID: 24037758
16. A hypoxia-induced decrease of PHLPP expression is attenuated by knocking down HIF1alpha but not HIF2alpha. PMID: 24061475
17. Data indicate that binding affinities for the PH domains of Akt and PHLPP1 were greater than for other PH domain-containing proteins, which may underlie the preferential recruitment of these proteins to membranes containing tocopherols. PMID: 23512990
18. we found that SGT1 was able to regulate the stability of PHLPP1, which is the direct phosphatase for Akt ser473 phosphorylation PMID: 23440515
19. USP46-mediated stabilization of PHLPP and the subsequent inhibition of Akt. PMID: 22391563
20. AG490 time-dependently down-regulated the protein expression of PHLPP and induced apoptosis of K562 cells PMID: 22931649
21. Studies suggest that the effects of PHLPP1 deletion on prostate carcinogenesis may be explained by the strong dependence of prostate cancer on phosphoinositide 3-kinase (PI3K)/Akt signalling. PMID: 22340730
22. Loss of PHLPP1 is associated with chronic lymphocytic leukemia. PMID: 22237780
23. a novel USP1-PHLPP1-Akt signaling axis, and decreased USP1 level in lung cancer cells may play an important role in lung cancer progress. PMID: 22426999
24. PHLPP1 has tumor suppressive activity and might represent a therapeutic or diagnostic tool for pancreatic ductal adenocarcinoma. PMID: 22044669
25. show for the first time a significant disruption of all three members of the PTEN-NHERF1-PHLPP1 tumor suppressor network in high-grade tumors, correlating with Akt activation and patient's abysmal survival PMID: 21804599
26. PHLPP as a novel tumor suppressor. PMID: 22144674
27. Knockdown of Scribble (Scrib) results in redistribution of PHLPP1 from the membrane to the cytoplasm and an increase in Akt phosphorylation. PMID: 21701506
28. codeletion of PTEN and PHLPP1 in patient samples is highly restricted to metastatic disease and tightly correlated to deletion of TP53 and PHLPP2 PMID: 21840483
29. Increased abundance of PHLPP-1, production of which is regulated by insulin, may represent a new molecular defect in insulin-resistant states such as obesity. PMID: 21461637
30. the cellular localization of beta-TrCP1 is altered in glioblastoma, resulting in dysregulation of PHLPP1 and other substrates such as beta-catenin. PMID: 21454620
31. a compensatory feedback regulation in which the activation of Akt is inhibited by up-regulation of PHLPP through mTOR, and this mTOR-dependent expression of PHLPP subsequently determines the rapamycin sensitivity of cancer cells. PMID: 21177869
32. Reduced expression of the tumor suppressor PHLPP1 enhances the antiapoptotic B-cell receptor signal in chronic lymphocytic leukemia B-cells. PMID: 20861921
33. Aberrantly expressed UCH-L1 boosts signaling through the Akt pathway by downregulating the antagonistic phosphatase PHLPP1, an event that requires its de-ubiquitinase activity PMID: 20574456
34. In the striatum, reduced levels of PHLPP1 can contribute to maintain high levels of activated Akt that may delay cell death and allow the recovery of neuronal viability after mutant huntingtin silencing PMID: 19745829
35. The PHLPP1 dephosphorylate Mst1 on the T387 inhibitory site, which activate Mst1 and its downstream effectors p38 and JNK to induce apoptosis. PMID: 20513427
36. Mechanism to selectively terminate Akt-signaling pathways through the differential inactivation of specific Akt isoforms by specific PHLPP isoforms. PMID: 17386267
37. PHLPP controls the cellular levels of PKC by specifically dephosphorylating the hydrophobic motif, thus destabilizing the enzyme and promoting its degradation. PMID: 18162466
38. Study focuses on the function of PHLPP1 and PHLPP2 in modulating signaling by Akt and PKC. PMID: 18511290
39. Loss of PHLPP expression is associated with colon cancer. PMID: 19079341
40. demonstrate a key role for beta-TrCP in controlling the level of PHLPP1, and activation of Akt negatively regulates this degradation process. PMID: 19797085
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亞細胞定位：

Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note=In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.; [Isoform 2]: Cell membrane.
組織特異性：

In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at pro
數據庫鏈接：

HGNC: 20610

OMIM: 609396

KEGG: hsa:23239

STRING: 9606.ENSP00000262719

UniGene: Hs.465337