Recombinant Human Nuclear RNA export factor 1 (NXF1)

中文名稱：

人NXF1重組蛋白
貨號：

CSB-YP016218HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人NXF1重組蛋白
貨號：

CSB-EP016218HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人NXF1重組蛋白
貨號：

CSB-EP016218HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人NXF1重組蛋白
貨號：

CSB-BP016218HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人NXF1重組蛋白
貨號：

CSB-MP016218HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

NXF1
Uniprot No.：

Q9UBU9
別名：

DKFZp667O0311; DmNXF1; MEX67; MEX67, yeast, homolog of; Mex67p; mRNA export factor TAP; Mvb1; Nuclear RNA export factor 1; Nuclear RNA export factor 1 homolog (S. cerevisiae); nxf 1; NXF1; NXF1_HUMAN; Protein small bristles; Sbr; TAP; Tip associating protein; Tip-associated protein; Tip-associating protein
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-619
氨基酸序列

ADEGKSYSE HDDERVNFPQ RKKKGRGPFR WKYGEGNRRS GRGGSGIRSS RLEEDDGDVA MSDAQDGPRV RYNPYTTRPN RRGDTWHDRD RIHVTVRRDR APPERGGAGT SQDGTSKNWF KITIPYGRKY DKAWLLSMIQ SKCSVPFTPI EFHYENTRAQ FFVEDASTAS ALKAVNYKIL DRENRRISII INSSAPPHTI LNELKPEQVE QLKLIMSKRY DGSQQALDLK GLRSDPDLVA QNIDVVLNRR SCMAATLRII EENIPELLSL NLSNNRLYRL DDMSSIVQKA PNLKILNLSG NELKSERELD KIKGLKLEEL WLDGNSLCDT FRDQSTYISA IRERFPKLLR LDGHELPPPI AFDVEAPTTL PPCKGSYFGT ENLKSLVLHF LQQYYAIYDS GDRQGLLDAY HDGACCSLSI PFIPQNPARS SLAEYFKDSR NVKKLKDPTL RFRLLKHTRL NVVAFLNELP KTQHDVNSFV VDISAQTSTL LCFSVNGVFK EVDGKSRDSL RAFTRTFIAV PASNSGLCIV NDELFVRNAS SEEIQRAFAM PAPTPSSSPV PTLSPEQQEM LQAFSTQSGM NLEWSQKCLQ DNNWDYTRSA QAFTHLKAKG EIPEVAFMK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.
基因功能參考文獻：
1. Gain or loss-of-function experiments revealed NXF1 selectively regulates TLR7-driven IRF5 transcriptional activity, suggesting a new role for NXF1 in the IRF5 signaling pathway. PMID: 28578407
2. repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats PMID: 28677678
3. Hepatitis B virus core protein associates with the cellular NXF1-p15 complex via the nuclear export signal motif of hepatitis B virus core protein.Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the NXF1-p15 machinery. PMID: 25360769
4. Complementary structural, biochemical and cellular techniques indicated that the formation of a symmetric RNA binding platform generated by dimerization of NXF1:NXT1 facilitates the recognition of CTE-RNA and promotes its nuclear export PMID: 25628361
5. Gag polyprotein synthesis was decreased by NXF1 knockdown. PMID: 25212909
6. TREX is the major complex used to recruit Nxf1 to mRNA in human cells. PMID: 22893130
7. the organization of the NXF1 proline-tyrosine nuclear localization signal reveal unexpected redundancy in the nuclear import pathways used by NXF1. PMID: 21965294
8. The U2AF65 protein served as an adaptor to link expanded CAG RNA to NXF1 for RNA export. PMID: 21725067
9. The crystal structure of the RNA recognition and leucine-rich repeat motifs of TAP bound to one symmetrical half of the CTE RNA is solved. PMID: 21822283
10. these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID: 21299467
11. Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway. PMID: 21613532
12. These results showed that the interaction between herpes simplex virus type 1 ICP27 and human TAP/NXF1 occurred in living cells upon head-to-tail intramolecular association of ICP27. PMID: 21060739
13. Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway PMID: 19924249
14. Depletion of NXF1 or 5,6-dichloro-1-beta-d-ribofuranosyl-benzimidazole treatment had similar effects, inhibiting the nuclear export of several of the H5N1 influenza virus mRNAs. PMID: 20071484
15. The structural and mutational data define a novel RNA-binding site on the Tap protein PMID: 11854490
16. Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1 PMID: 11875519
17. Karyopherin beta2B is a nuclear export factor and mediates mRNA export in cooperation with TAP PMID: 12384575
18. structural basis for interaction of UBA domain and FG nucleoporins PMID: 12581645
19. nuclear export sequences regulate human TAP function in mRNA export by direct nuclear pore interactions PMID: 14963046
20. hypophosphorylation of ASF in mRNPs coincides with its higher affinity for TAP PMID: 15184380
21. SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated PMID: 15210956
22. concluded that herpes simplex virus type 1 ICP27 and the viral RNAs to which it binds are exported via the TAP/NXF1 export receptor. PMID: 15767397
23. results indicate that Tap regulates expression of its own intron-containing RNA through a CTE (constitutive transport element)-mediated mechanism PMID: 16971948
24. RNA transport element RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts PMID: 17001072
25. evidence presented that the export factor TAP/NXF1 binds poorly to XIST RNA in comparison to exported mRNAs, suggesting that reduced TAP/NFX1 binding may contribute to nuclear retention of XIST RNA PMID: 17333237
26. ORF57 interacts with the cellular export factor REF and with RNA, suggesting that it may provide target mRNAs with access to REF, which mediates nuclear RNA export by binding to TAP/NXF1. PMID: 17609285
27. Homotypic Tap complex can interact with both Nxt1 and nucleoporins in vitro. PMID: 17978099
28. The efficiency and stability of the approach are demonstrated by reconstructing the structure of a two domain region of the 31 kDa nuclear export factor TAP (TIP-associated protein). PMID: 18670889
29. ICP27 is the major export adaptor for HSV-1 mRNA and that it links bound transcripts to the TAP/NXF1 export receptor PMID: 19019956
30. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. PMID: 19586903
31. oligomerization of Tap affects its interactions with nucleoporins PMID: 17978099
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亞細胞定位：

Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Nucleus, nuclear pore complex. Nucleus envelope. Cytoplasm. Cytoplasm, Stress granule.
蛋白家族：

NXF family
組織特異性：

Expressed ubiquitously.
數據庫鏈接：

HGNC: 8071

OMIM: 602647

KEGG: hsa:10482

STRING: 9606.ENSP00000294172

UniGene: Hs.523739