Recombinant Human Mucolipin-3 (MCOLN3), partial

中文名稱：

人MCOLN3重組蛋白
貨號：

CSB-YP855055HU1
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人MCOLN3重組蛋白
貨號：

CSB-EP855055HU1
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人MCOLN3重組蛋白
貨號：

CSB-EP855055HU1-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人MCOLN3重組蛋白
貨號：

CSB-BP855055HU1
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人MCOLN3重組蛋白
貨號：

CSB-MP855055HU1
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MCOLN3
Uniprot No.：

Q8TDD5
別名：

MCOLN3; Mucolipin-3; Transient receptor potential channel mucolipin 3; TRPML3
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Nonselective ligand-gated cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity. Mediates release of Ca(2+) from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway. Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells. Proposed to play a critical role at the cochlear stereocilia ankle-link region during hair-bundle growth. Involved in the regulation of autophagy. Through association with GABARAPL2 may be involved in autophagosome formation possibly providing Ca(2+) for the fusion process. Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events. Possible heteromeric ion channel assemblies with TRPV5 show pharmacological similarity with TRPML3.
基因功能參考文獻：
1. these results suggest that TRPML3 plays a role in autophagosome maturation through the interaction with GATE16, by providing Ca(2+) in the fusion process. PMID: 24269818
2. TRPML3 and TRPV5 heteromers could have a biological function PMID: 23469151
3. Negatively charged amino acids in the extracellular loops of TRPML3 may interfere with the observed sodium inhibition. PMID: 22753890
4. TRPML 1, 2 and 3 assemblies regulated cell viability and starvation-induced autophagy. PMID: 20736310
5. analysis of the TRPML3 channel pore and its stable expansion by the Varitint-Waddler-causing mutation PMID: 20378547
6. there is a hierarchy controlling the subcellular distributions of the TRPMLs such that TRPML1 and TRPML2 dictate the localization of TRPML3 and not vice versa PMID: 16606612
7. The A419P mutation affects TRPLMe channel glycosylation and causes massive cell death PMID: 17962195
8. TRPML3(A419P) and (I362T+A419P) at physiological potentials may have a role in hair cell degeneration and deafness PMID: 18162548
9. First characterization of wild-type TRPML3 calcium-permeable channel properties and its regulation by extracytosolic (luminal)hydrogen ion (H+). PMID: 18369318
10. The deaf-waddler isoform of PMCA2, operating at 30% efficacy, showed a significantly decreased ability to rescue the Ca(2+) loading of cells expressing TRPML3(A419P). PMID: 19299509
11. Results show that mucolipin 3 is a novel calcium channel that plays a crucial role in the regulation of cargo trafficking along the endosomal pathway. PMID: 19497048
12. These findings reveal a prominent role for TRPML3 in regulating endocytosis, membrane trafficking and autophagy, perhaps by controlling the Ca(2+) in the vicinity of cellular organelles that is necessary to regulate these cellular events. PMID: 19522758
13. Data show that TRPMLs form distinct functional channel complexes. PMID: 19885840
顯示更多

收起更多
亞細胞定位：

Cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane.
蛋白家族：

Transient receptor (TC 1.A.4) family, Polycystin subfamily, MCOLN3 sub-subfamily
數據庫鏈接：

HGNC: 13358

OMIM: 607400

KEGG: hsa:55283

STRING: 9606.ENSP00000304843

UniGene: Hs.535239