Recombinant Human Mitogen-activated protein kinase kinase kinase 11 (MAP3K11), partial

中文名稱：

人MAP3K11重組蛋白
貨號：

CSB-YP013419HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人MAP3K11重組蛋白
貨號：

CSB-EP013419HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人MAP3K11重組蛋白
貨號：

CSB-EP013419HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人MAP3K11重組蛋白
貨號：

CSB-BP013419HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人MAP3K11重組蛋白
貨號：

CSB-MP013419HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MAP3K11
Uniprot No.：

Q16584
別名：

2610017K16Rik; EC 2.7.11.25; M3K11_HUMAN; Map3k11; MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1 ; PTK1; RHOE; SH3 domain containing proline rich kinase ; SPRK; Src-homology 3 domain-containing proline-rich kinase
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
基因功能參考文獻：
1. These results suggest that in the early response to stressful stimuli, MLK4beta-MLK3 binding is important for regulating MLK3 activity and MAPK signalling, and after prolonged periods of stress exposure, MLK4beta and MLK3 proteins decline via CHIP-dependent degradation. PMID: 28757353
2. In summary, this investigation identifies an EGFR-DOCK180-RAC1-MLK3-JNK signaling axis that drives glioblastoma cell migration and dissemination.IMPLICATIONS: On the basis of these findings, MLK3 emerges as a potential therapeutic target for the treatment of glioblastoma PMID: 28487380
3. chronic hypoxia can reduce MLK3 expression in a posttranscriptional regulatory manner. PMID: 28555332
4. Data indicate that BTG2, MAP3K11, RPS6KA1 and PRDM1 as putative targets of microRNA miR-125b. PMID: 27613090
5. Increased expression of MAP3K11 is associated with esophageal cancer. PMID: 26717044
6. MLK3 serves as a common upstream kinase of AMPK and JNK and functions as a direct upstream kinase for AMPK independent of LKB1 PMID: 25874865
7. MLK3 represents a newly recognized integral component of HER2 biology in HER2+ breast tumors. PMID: 26152725
8. MLK3 is a critical factor controlling the activity of kinase networks that control the cellular responses to different concentrations of reactive oxygen species. PMID: 24894995
9. Signaling pathways associated with the Pro252His mutation in MLK3 are located in the kinase domain which is an important domain for the regulation of downstream signaling pathways. PMID: 24628919
10. CHIP modulates MLK3 protein levels in response to Geldanamycin and stress stimuli, and CHIP-dependent regulation of MLK3 is required for suppression of SKOV3 ovarian cancer cell invasion. PMID: 24912674
11. Data indicate URMC-099 as an orally bioavailable, potent mixed lineage kinase 3 MLK3 inhibitor. PMID: 24044867
12. NS5A targets MLK3 with multiple downstream consequences for both apoptosis and K(+) homeostasis. PMID: 23857585
13. Lysine 63-linked ubiquitination modulates mixed lineage kinase-3 interaction with JIP1 scaffold protein in cytokine-induced pancreatic beta cell death PMID: 23172226
14. High MAP3K11 expression is associated with prostate cancer. PMID: 22761715
15. Findings suggest that the MLK3-JNK-paxillin signaling axis may represent a potential prognostic marker in breast cancer metastasis. PMID: 22700880
16. Data suggest that MEKK2 and MLK3 represent untargeted kinases in tumor biology for potential therapeutic development. PMID: 22139075
17. The findings establish MLK3 as an important regulator of MMP expression and invasion in ovarian cancer cells. PMID: 22652451
18. These findings collectively suggest that the MLK3-Pin1 signaling cascade plays a critical role in regulating the cell cycle, centrosome numbers, and oncogenesis. PMID: 22566623
19. Data show that HBx can induce HepG2 cell apoptosis via a novel active MLK3-MKK7-JNKs signaling module to upregulate FasL protein expression. PMID: 22509080
20. co-expression of MLK3 and beta-catenin resulted in significant G(2)/M arrest. PMID: 21880738
21. These results establish merlin as a potent inhibitor of mixed lineage kinase 3, extracellular signal-regulated kinase, and c-jun N-terminal kinase activation in cancer. PMID: 20890305
22. MLK3 is critical for breast cancer cell migration and promotes cell transformation. PMID: 20514022
23. Studies indicate that the MLK3/JNK1 axis mediates G17-induced gastric cancer cell migration, which can be targeted for designing novel therapeutic strategies for treating gastric malignancies. PMID: 20150185
24. Novel proteolytic processing of MLK3 may negatively control MLK3 signalling to JNK. PMID: 20158498
25. MLK3 mutations is associated with the mismatch repair deficiency in gastrointestinal cancer. PMID: 19955118
26. Estradiol inhibits the proapoptotic function of MLK3 as a mechanism to limit cytotoxic drug-induced death of ER(+) breast cancer cells. PMID: 20145118
27. MLK3 functions to limit IKK activity, and depleting MLK3 helps protect cells from etoposide-induced cell death through activation of IKK-dependent signaling. PMID: 19782705
28. MLK3 may be a negative regulator of the growth-promoting and transforming properties of Rac1 PMID: 11713255
29. Twelve in vivo MLK3 phosphorylation sites have been identified. PMID: 11969422
30. negative regulation by protein kinase B leads to cell survival PMID: 12458207
31. ceramide and TNF-alpha are agonists PMID: 12504027
32. MLK3 is phosphorylated by Akt2, which results in the disassembly of the JNK complex bound to POSH and down-regulation of the JNK signaling pathway PMID: 14504284
33. MLK3 signaling requires Hsp90/p50cdc37 PMID: 15001580
34. These observations support a role for mixed lineage kinases, including MLK3, in the TGF-beta1-induced cell death mechanism. PMID: 15069087
35. MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation. PMID: 15258589
36. The MLK3 might be a unique target to selectively inhibit transformed cell proliferation by disrupting mitotic spindle formation resulting in mitotic arrest. PMID: 15923109
37. Cdc42 induces activation loop phosphorylation and membrane targeting of mixed lineage kinase 3 PMID: 16253996
38. Merlin and MLK3 can interact in situ and merlin can disrupt the interactions between B-Raf and Raf-1 or those between MLK3 and either B-Raf or Raf-1. PMID: 16537381
39. JNK-mediated feedback phosphorylation of MLK3 regulates its activation and deactivation states by cycling between Triton-soluble and Triton-insoluble forms PMID: 16687404
40. a direct link between GSK-3beta and MLK3 activation in a neuronal cell death pathway and identify MLK3 as a direct downstream target of GSK-3beta. PMID: 17711861
41. p38 MAPK inhibitors SB202190 and SB203580 activated JNK via MLK-3/MKK7 pathway. PMID: 18222647
42. findings demonstrate a scaffolding role for MLK3 in controlling the extent of Rho activation that modulates cell migration PMID: 18851832
43. K48-linked ubiquitination directs Mixed lineage kinase 3 for proteosomal degradation while K63-linked ubiquitination is important for Mixed lineage kinase 3 kinase activity. PMID: 19586614
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Location is cell cycle dependent.
蛋白家族：

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
組織特異性：

Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
數據庫鏈接：

HGNC: 6850

OMIM: 600050

KEGG: hsa:4296

STRING: 9606.ENSP00000309597

UniGene: Hs.502872