Recombinant Human Melanocyte-stimulating hormone receptor (MC1R)-VLPs

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中文名稱：

人MC1R重組蛋白
品名簡稱：

Recombinant Human MC1R protein-VLPs
貨號：

CSB-MP086369HU
說明書：

MSDS Datasheet
規格：

￥5250
圖片：
CSB-MP086369HU is detected by Mouse anti-6*His monoclonal antibody.

The purity of VLPs was greater than 95% as determined by SEC-HPLC
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SEC-HPLC.
生物活性：

Not Test
基因名：

MC1R
Uniprot No.：

Q01726
別名：

MSH-R;Melanocortin receptor 1;MC1-R
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Mammalian cell
分子量：

36.1 kDa
表達區域：

1-317aa
氨基酸序列

MAVQGSQRRLLGSLNSTPTAIPQLGLAANQTGARCLEVSISDGLFLSLGLVSLVENALVVATIAKNRNLHSPMYCFICCLALSDLLVSGSNVLETAVILLLEAGALVARAAVLQQLDNVIDVITCSSMLSSLCFLGAIAVDRYISIFYALRYHSIVTLPRARRAVAAIWVASVVFSTLFIAYYDHVAVLLCLVVFFLAMLVLMAVLYVHMLARACQHAQGIARLHKRQRPVHQGFGLKGAVTLTILLGIFFLCWGPFFLHLTLIVLCPEHPTCGCIFKNFNLFLALIICNAIIDPLIYAFHSQELRRTLKEVLTCSW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions)
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Mediates melanogenesis, the production of eumelanin (black/brown) and phaeomelanin (red/yellow), via regulation of cAMP signaling in melanocytes.
基因功能參考文獻：
1. the association between particular MC1R alleles and the risk of skin cancers, was investigated. PMID: 29968341
2. there is an association between TERT promoter mutations and MC1R variants in melanoma patients PMID: 27930874
3. with regard to cutaneous malignant melanoma risk, no association found in the combination of GSTP1 Ile105Val with MC1R RHC-variant alleles [meta-analysis] PMID: 27304781
4. In familial melanoma patients, the presence of MC1R R variants was associated with an increased prevalence of environmental risk factors and features associated with UV radiation-induced damage. PMID: 29405243
5. In melanomas and for somatic C>T mutations, a signature linked to sun exposure, the expected single-nucleotide variant count associated with the presence of an R allele is estimated to be 42% (95% CI, 15-76%) higher than that among persons without an R allele. PMID: 27403562
6. These data clearly show a new and hitherto unsuspected role for MC1R in noncutaneous solid tissues before birth. PMID: 29316344
7. In both melanoma cell lines, alphaMSH determined the reduction of proliferation through the PI(4,5)P2/PLC pathway, employing PPARgamma as an effector element. These evidence could offer perspectives for new therapeutic approaches for melanoma. PMID: 29020973
8. Carriage of any MC1R variant, one variant and two or more variants, compared with not having such variants was significantly associated with fair hair color, skin type I/II, and presence of freckles. PMID: 27251790
9. key molecular events driving MC1R-mediated enhancement of genome maintenance and MC1R-induced pigment induction in melanocytes are distinct PMID: 28094871
10. amino acid residue 128 in Transmembrane 3 (TM3) of MC1R, or amino acid residue L133 in TM3 of the MC4R, play crucial roles in ligand des-Trp(9)-NDP-alpha-MSH selectivity at MC1R or MC4R. PMID: 28579117
11. In a heterologous expression system, MC1R-dependent Arrestins B ubiquitination was enhanced by overexpression of MGRN1 and was impaired by siRNA-mediated MGRN1 knockdown thus pointing to MGRN1 as the responsible E3-ligase. PMID: 28947386
12. POMC and MC1R were significantly lower in vitiligo lesional skin than in non-lesional skin as well as in controls and they were significantly higher in non-lesional skin than in the skin of the controls. PMID: 26437741
13. MC1R gene could modify the age of onset in Spanish Huntington's disease patients. PMID: 27924526
14. results highlight a central role for MC1R palmitoylation in pigmentation and protection against melanoma PMID: 28869973
15. MC1R variant p.V92M (rs2228479) was present in 72 (14%) patients and 15 (9%) controls and confers increased risk of developing late-onset Alzheimer's disease, especially in those patients whose genetic risk could not be explained by APOE genotype. This association remains and even increased in the subset of 69 patients with typical AD cerebrospinal fluid profile. No association was found between p.V92M and age of onset. PMID: 28059796
16. MC1RR163Q is associated with a lower risk of complicated sepsis after trauma. Therapeutic targeting of MC1R may be beneficial for trauma patients at risk for complicated sepsis PMID: 27488084
17. This study evaluated the association of perceived facial age with multiple single nucleotide polymorphisms in the MC1R gene. PMID: 27133870
18. MC1R-positive basophils are present exclusively in the nasal mucosa of those AR patients who had undergone nasal allergen provocation 24 h before. PMID: 27196703
19. MC1R genotype is associated with patient phenotypes with BRAF and NRAS mutations in melanoma PMID: 28842324
20. MC1R mutation is associated with Melanoma. PMID: 28024115
21. Melanocortin-1 receptor variants are associated with high melanoma risk. PMID: 27540956
22. It was suggested to be involved in Parkinson disease (PD) and melanoma, but its genetic variants do not play roles in susceptibility for Parkinson disease or rapid eye movement sleep behavior disorder. PMID: 27131830
23. Variants in MC1R do not play a major role in PD in the Chinese population. PMID: 27084066
24. Latin American families had CDKN2A mutations more frequently (P = 0.014) than Spanish ones. Of patients with SMP, 10% of those from Latin America and 8.5% of those from Spain had mutations in CDKN2A (P = 0.623). The most recurrent CDKN2A mutations were c.-34G>T and p.G101W. Latin American patients had fairer hair and skin) and a higher prevalence of MC1R variants compared with Spanish ones PMID: 26681309
25. MC1R genetic effects might contribute to sex-specific differences in skin phototype PMID: 27637409
26. MC1R may represent a common pathogenic pathway for melanoma and Parkinson disease. PMID: 28019657
27. Carriers of MC1R variants were at increased melanoma risk independent of their sun exposure. PMID: 27050141
28. This study confirms the presence of CDKN2A and MC1R variants among Cypriot melanomas and supports existing evidence of a role for these variants in susceptibility to melanoma. PMID: 28360400
29. Presence of MC1R variant alleles predicted higher levels of dental fear and fear of pain. Importantly, fear of pain mediated the relation between MC1R variant status and dental fear. PMID: 27555332
30. this case-control genetic association study using Taiwanese population showed that certain linkage disequilibrium structure of MC1R is strongly associated with vitiligo PMID: 26670452
31. Case Report: MC4R p.Met215del coexisting with FTO and MC1R gene variants, causes severe early onset obesity. PMID: 27394708
32. MC1R status is associated with BRAF(V600E), BRAF(V600K), and NRAS mutations in cutaneous melanomas PMID: 26807515
33. Study found that HBD2 bound tightly to Mc1r with an affinity similar to that of HBD3 PMID: 26536271
34. Six MC1R mutations segregated with the phenotype in seven families, including a p.Val174del in-frame deletion and a p.Tyr298* nonsense mutation, that were analyzed for function in heterologous HEK293 cells. PMID: 26197705
35. Even after taking into account potential confounding effects of MC1R genotype and various lifestyle factors that close relatives may share, family history of skin cancer remained strongly associated with early-onset basal cell carcinoma PMID: 26381319
36. Woman who carried a deleterious CDKN2A mutation and was also homozygous for the MC1R variant allele R160W developed 20 cutaneous melanomas in her lifetime. PMID: 26103950
37. The pattern of sequence diversity here does not support a model of strong selective constraint on MC1R in Northern Island Melanesia. PMID: 26482799
38. Functional characterization of MC1R-TUBB3 intergenic splice variants of the human melanocortin 1 receptor has been undertaken in response to ultraviolet irradiation. PMID: 26657157
39. none of the three variants of the MC1R gene and related haplotypes be associated with sporadic form of Parkinson disease in Chinese Han population from Mainland China. PMID: 26628245
40. MC1R genotype is a risk factor for the development of BCC in Greek patients independently of pigmentary characteristics, and the combination of MC1R variants may modulate this risk. PMID: 25809071
41. Interestingly, we observed an increased risk of melanoma in subjects with darker skin and lower nevus count, usually considered at low risk, when carrying melanocortin 1 receptor polymorphisms. PMID: 25736238
42. MC2R and MC1R signals are consecutively required for the regulation of EPO signal transduction in erythroblast differentiation, and MC5R signal transduction is required to induce enucleation. PMID: 25860801
43. MC1-signaling protects against cartilage degradation and subchondral bone sclerosis in OA indicating a beneficial role of the POMC system in joint pathophysiology. PMID: 25191747
44. The presence of two common variants of MC1R was identified as a risk factor for severe photoaging. PMID: 26067307
45. ASIP diminishes agonist-independent MC1R basal signaling whereas HBD3 is a neutral MC1R antagonist that blocks activation by melanocortins. PMID: 26168232
46. the inhibitory effects of alpha-MSH on inflammation are dependent on MC-1 R PMID: 25898835
47. Our results suggest inherited variation in MC1R may play an influential role in anatomic site presentation of melanomas and may differ with respect to skin pigmentation phenotype. PMID: 25790105
48. The computational analysis proved capable of identifying the potentially damaging non-synonymous single nucleotide polymorphisms in MC1R. PMID: 25794181
49. MC1R mutations in this series demonstrated and inverse association for head and neck melanomas. PMID: 25385688
50. MC1R polymorphism is associated in patients with pot-burn hypertrophic scarring. PMID: 26030184
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相關疾病：

Melanoma, cutaneous malignant 5 (CMM5)
亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Expressed in melanocytes. Expressed in corticoadrenal tissue.
數據庫鏈接：

HGNC: 6929

OMIM: 155555

KEGG: hsa:4157

STRING: 9606.ENSP00000451605

UniGene: Hs.513829