Recombinant Human Lupus La protein (SSB)

^{In
Stock}

中文名稱：

人SSB重組蛋白
品名簡稱：

Recombinant Human SSB protein
貨號：

CSB-EP022705HU
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

SSB
Uniprot No.：

P05455
別名：

Autoantigen La; La ; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family member 3; LA_HUMAN; LARP3; Lupus La antigen; Lupus La protein; Lupus La protein homolog ; MGC118101; MGC93380; mRNA for autoantigen; OTTMUSP00000014043; RP23 273G23.1; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B (autoantigen La); Sjogren syndrome antigen B; SS B; SS-B; SS-B/La protein ; SSB
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

62.8kDa
表達區(qū)域：

1-408aa
氨基酸序列

MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

A cDNA encoding residues Met1 to Gln408 of the human Lupus La protein/SSB full-length protein was fused with a 6xHis-SUMO-tag at the N-terminus and then expressed in E.coli. The forming recombinant protein was validated by SDS-PAGE. Its purity is greater than 90%. On the reducing SDS-PAGE gel, the SSB protein migrated to the molecular mass band of approximately 65 kDa. And recombinant SSB proteins in-stock are available. This recombinant SSB protein may act as the immunogen to generate specific antibodies or be used in SSB-related epigenetic and nuclear signal transduction.

The Lupus autoantigen SSB is an RNA-binding protein implicated in the stabilization of RNA polymerase III transcripts, support of RNA folding, and mammalian miRNA pathway. Acting as a gatekeeper, SSB is responsible for proper tRNA maturation and protection of the miRNA pathway from potentially functional tRNA fragments. Pathogenesis of autoimmune diseases such as systemic lupus erythematosus (SLE) and Sjögren’s syndrome (SS) is associated with SSB.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
基因功能參考文獻：
1. A cytoplasmic isoform of La protein as well as LARPs 6, 4, and 1 function in mRNA metabolism and translation in distinct but similar ways, sometimes with the poly(A)-binding protein, and in some cases by direct binding to poly(A)-RNA. PMID: 28782243
2. our data support a novel model, whereby cancer-associated La protein contributes to cisplatin resistance by stimulating the translation of anti-apoptotic factor Bcl2 in HNSCC cells. PMID: 27105491
3. La protein is an important microprocessor component regulating miRNA processing efficiency by association with DGCR8 to regulate formation of the DGCR8-Drosha complex for miRNA processing. PMID: 29087193
4. La functions as gatekeeper ensuring correct tRNA maturation and protecting the miRNA pathway from potentially functional tRNA fragments. PMID: 27345152
5. Findings identified two novel SUMO acceptors sites on La and suggest that sumoylation of La alters its RNA-binding activity, which could influence a broad range of RNA processing steps where La's function has been implicated. PMID: 27224031
6. Binding of double-stranded RNA involves less penetration into the RNA recognition motif (RRM2) binding pocket and more engagement with the unstructured C-terminus of the La C-terminal domain (CTD). PMID: 27959512
7. At the 3' UTR, HUR facilitates circularization of the viral genome through interaction with another host factor, La, which is critical for replication. PMID: 26339049
8. expression of nuclear hLa Ag induces thymic clonal deletion and tTreg selection, and lymphocytic infiltration of the lung is a consequence of La-specific CD4(+) T cell autoimmunity PMID: 25582858
9. Authors provide a model whereby a novel interplay between RNA-binding, RNA chaperoning and AKT phosphorylation of La protein regulates CCND1 IRES-mediated translation. PMID: 25520193
10. Primary Sjogren's patients with WSP form a benign subgroup, with a lower prevalence of anti-SSB and extraglandular manifestations. PMID: 24529195
11. A beta turn in the human La protein is responsible for the interaction with the hepatitis C virus RNA. PMID: 24478427
12. Total IgG levels correlate positively with autoantibodies La, Ro60 and Ro52 but not with RING or B-box domain antibodies of Ro52. PMID: 23554036
13. The competition between the host factor (La) and the viral protein (NS3) for binding to HCV IRES might regulate the molecular switch from translation to replication of HCV. PMID: 22355520
14. Our study demonstrates that La plays a critical role in regulating HCV replication by interacting with the GCAC motif. PMID: 23552417
15. Sjogren syndrome antigen B (SSB)/La promotes global microRNA expression by binding microRNA precursors through stem-loop recognition PMID: 23129761
16. Iron promotes the translation initiation of hepatitis C virus by stimulating the expression of eIF3A and La proteins. PMID: 22634302
17. oxidants cause nuclear export of La/SSB protein and subsequent association of La/SSB with Nrf2 untranslated regions and ribosomes PMID: 22207702
18. data indicate that the JAK2(V617F) mutation affects p53 response to DNA damage through the upregulation of La antigen and accumulation of MDM2 PMID: 21785463
19. the structural elements required for La-mediated RNA chaperone activity PMID: 22203678
20. La binds to domain IV of the hepatitis C virus RNA using an RNA recognition that is quite distinct from its mode of binding to RNAs with a 3' UUU(OH) trailer. PMID: 22009680
21. function of the RNA-binding protein La in promoting tumor progression of head and neck squamous cell carcinoma PMID: 22016766
22. Autoantigen La promotes efficient RNAi, antiviral response, and transposon silencing by facilitating multiple-turnover RNA-induced silencing complex catalysis. PMID: 22055194
23. Overexpression of mouse or human La enhanced M-MuLV particle release in the absence of glycosylated gag. PMID: 21343359
24. La protein binds to and mediates interaction of the 5' and 3' noncoding ends of the Japanese encephalitis virus genome. PMID: 21294637
25. show elevated La protein expression in cervical cancer tissue and its correlation with aberrant CCND1 protein levels in cervical tumor tissue lysates PMID: 20856207
26. There is a correlation between La protein and HBV mRNA and the expression of HBV protein. PMID: 17064465
27. study found expression of La protein is induced by HCV infection, and this induced La protein-activated telomerase activity in a hepatoma cell line; HCV infection might be strongly related to telomerase activity in the liver through La protein induction PMID: 20497049
28. nuclear trafficking by La affects the normal order of pre-tRNA processing PMID: 12049746
29. molecular characterization of the human La protein.hepatitis B virus RNA.B interaction in vitro PMID: 12121976
30. La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA PMID: 12384597
31. binds differentially to multiple sites within the 5' untranslated region of Coxsackievirus B3 RNA PMID: 12457960
32. in addition to full-length La protein, both N- and C-terminal halves were able to interact with hepatitis C virus internal ribosome entry site in vivo. PMID: 12540850
33. Dengue 4 virus minus strand 3'UTR RNA binds with La protein in human monocytes PMID: 12584332
34. Results suggest that La ribonucleoproteins (RNP) exist in distinct states that differ in subcellular localization, serine 366 phosphorylation, and associated RNAs. PMID: 14636586
35. La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices PMID: 15004549
36. La protein binds to the 5' end and with the 3' UTR of Dengue virus RNA; interacts with the dengue virus nonstructural proteins NS5 and NS3 PMID: 15084396
37. NMR assignment and secondary structure of the La motif PMID: 15213463
38. Composition of La/hepatitis B virus ribonucleoprotein particles as well as interacting cellular factors, are critical determinants in the regulation of the stability of the HBV RNA. PMID: 15302879
39. RRM1 and La motifs bind pre-tRNA PMID: 15371415
40. CK2 is responsible for La S(366) phosphorylation in vivo PMID: 15485924
41. Nonphosphorylated SSB interacts with nucleolin at nucleolar sites involved in rRNA biogenesis. PMID: 15572691
42. This protein is s host factors responsible for the regulation of the hepatitis C virus internal ribosome entry site. PMID: 15685555
43. essential for efficient hepatitis C virus replication PMID: 15823607
44. In vitro RNA chaperone activity pf SSB was studied. PMID: 15928345
45. nuclear retention of peptidylglycine alpha-amidating monooxygenase (PAM) mRNA is lost upon expressing the La proteins that lack a conserved nuclear retention element, suggesting a direct association between PAM mRNA and La protein in vivo PMID: 16107699
46. Anti-La antibodies bound to immunodominant epitopes of La within the NH(2)-terminus and the RNA recognition motif (RRM) region of apoptotic human cells PMID: 16320341
47. Here, we show by chromatin immunoprecipitation (ChIP) that La is associated with pol III-transcribed genes in vivo PMID: 16344466
48. Results report the crystal structure of the N-terminal domain of human La, consisting of La and RRM1 motifs, bound to RNA. PMID: 16387655
49. Changes in salivary production rate are associated with aging and SSB antibodies. PMID: 16467037
50. La protein undergoes nucleocytoplasmic shuttling whereas EBER1 and EBER2 noncoding RNA's are confined to the nucleus. PMID: 16682524
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亞細胞定位：

Nucleus.
數(shù)據(jù)庫鏈接：

HGNC: 11316

OMIM: 109090

KEGG: hsa:6741

STRING: 9606.ENSP00000260956

UniGene: Hs.632535