Recombinant Human Histone-lysine N-methyltransferase 2B (KMT2B), partial

中文名稱：

人KMT2B重組蛋白
貨號：

CSB-YP890939HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人KMT2B重組蛋白
貨號：

CSB-EP890939HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人KMT2B重組蛋白
貨號：

CSB-EP890939HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人KMT2B重組蛋白
貨號：

CSB-BP890939HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人KMT2B重組蛋白
貨號：

CSB-MP890939HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

KMT2B
Uniprot No.：

Q9UMN6
別名：

Histone-lysine N-methyltransferase 2B; HRX2; KIAA0304 gene product; KMT2B; KMT2B_HUMAN; Likely ortholog of mouse WW domain binding protein 7 ; Lysine N-methyltransferase 2B; mixed lineage leukemia 4; Mixed lineage leukemia gene homolog 2 protein ; MLL2; myeloid lymphoid leukemia 4; Myeloid lymphoid or mixed lineage leukemia protein 4; Myeloid/lymphoid or mixed-lineage leukemia protein 4; Trithorax homolog 2; trithorax homologue 2 ; TRX2; WBP-7; WBP7; WW domain-binding protein 7
種屬：

Homo sapiens (Human)
蛋白長度：

partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place. Likely plays a redundant role with KMT2C in enriching H3K4me1 marks on primed and active enhancer elements. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
基因功能參考文獻：
1. Gene silencing experiments of MLL4 and the subunits PA1 and PTIP confirm TGF-beta-specific genes to be regulated by the MLL4 complex, which links TGF-beta signaling to transcription regulation by the MLL4 methyltransferase complex. PMID: 28976802
2. Summary of variants in KMT2B associated with dystonia, as well as the clinical phenotype (review) PMID: 29289525
3. Rare missense variation in KMT2B represents an additional cause of generalized dystonia. PMID: 28520167
4. In the univariate analyses, TP53, PPP1R3A, and KMT2B were significantly more frequently mutated in interval cancers than in screen-detected cancers. PMID: 27587435
5. we describe a method to seamlessly modify a putative CDK2 phosphorylation site on MLL2 to restrict its phosphorylation and activation. Specifically, by utilizing dimeric CRISPR RNA-guided nucleases, RFNs (commercially known as the NextGENtrade mark CRISPR), in combination with an excision-only piggyBactrade mark transposase, we demonstrate how to generate a point mutation of threonine-542, a predicted site to prevent M... PMID: 27075976
6. The crucial role of KMT2B in the physiological control of voluntary movement. PMID: 27992417
7. MLL4 mutation along with BRCA1 mutation confers chemoresistance in breast cancer. PMID: 28124401
8. Results show that KMT2B interacts with ERalpha to bind the ERalpha-binding sites of IL-20 and other ERalpha target genes with H3K4 modifications suggesting an important role for KMT2B in the epigenetic transcriptional regulation of cytokine IL-20, and other ERalpha-responsive genes, in breast cancer cells. PMID: 27806114
9. findings thus establish generalized dystonia as the human phenotype associated with haploinsufficiency of KMT2B; moreover, we provide evidence for a causative role of disordered histone modification, chromatin states, and transcriptional deregulation in dystonia pathogenesis PMID: 27839873
10. The results explain how the MLL SET domains of MLL1 and MLL4 are able to add multiple methyl groups to the target histone H3 lysine. PMID: 26320581
11. The Aven RGG/RG motif bound G4 structures within the coding regions of the MLL1 and MLL4 mRNAs increasing their polysomal association and translation, resulting in the induction of transcription of leukemic genes. PMID: 26267306
12. We propose that MLL3 and MLL4 are broadly required for controlling MAFA and MAFB transactivation during development and postnatally. PMID: 26180087
13. HBV-MLL4 integration occurred frequently in Chinese HCC patients, representing a unique molecular segment for HCC with HBV infection PMID: 25901726
14. Chromosomal translocation in a pediatric undifferentiated spindle cell sarcoma have characterized this alteration to show rearrangement of the MLL4 and GPS2 genes, resulting in fusion gene MLL4-GPS2, the expression of which promotes independent growth. PMID: 25139254
15. KMT2B transgene mediates hippocampal histone 3 lysine 4 di- and trimethylation and is a critical player for memory formation. PMID: 23426673
16. knockdown of MLL4 severely affects cell-cycle progression and induces apoptotic cell death in cultured tumour cells. PMID: 22713656
17. Alterations of the CxxC domain preclude oncogenic activation of mixed-lineage leukemia 2 PMID: 19060922
18. data suggest that the translocation breakpoint of MLL4 gene is one of the preferential targets for HBV DNA integration into the MLL4 gene and the HBV DNA integration may be involved in liver oncogenesis PMID: 18320596
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亞細胞定位：

Nucleus.
蛋白家族：

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, TRX/MLL subfamily
組織特異性：

Widely expressed. Highest levels in testis. Also found in brain with higher expression in the cerebellum than in any other region, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocyte
數據庫鏈接：

HGNC: 15840

OMIM: 606834

KEGG: hsa:9757

STRING: 9606.ENSP00000222270

UniGene: Hs.676457