Recombinant Human Histone RNA hairpin-binding protein (SLBP)

中文名稱：

人SLBP重組蛋白
貨號：

CSB-YP613502HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人SLBP重組蛋白
貨號：

CSB-EP613502HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人SLBP重組蛋白
貨號：

CSB-EP613502HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人SLBP重組蛋白
貨號：

CSB-BP613502HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人SLBP重組蛋白
貨號：

CSB-MP613502HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SLBP
Uniprot No.：

Q14493
別名：

Hairpin binding protein histone; HBP; Histone binding protein; Histone RNA hairpin binding protein; Histone RNA hairpin-binding protein; Histone stem-loop-binding protein; SLBP; SLBP_HUMAN; Stem loop (histone) binding protein; Stem loop binding protein
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-270
氨基酸序列

MACRPRSPPR HQSRCDGDAS PPSPARWSLG RKRRADGRRW RPEDAEEAEH RGAERRPESF TTPEGPKPRS RCSDWASAVE EDEMRTRVNK EMARYKRKLL INDFGRERKS SSGSSDSKES MSTVPADFET DESVLMRRQK QINYGKNTIA YDRYIKEVPR HLRQPGIHPK TPNKFKKYSR RSWDQQIKLW KVALHFWDPP AEEGCDLQEI HPVDLESAES SSEPQTSSQD DFDVYSGTPT KVRHMDSQVE DEFDLEACLT EPLRDFSAMS
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.
基因功能參考文獻：
1. SLBP is a potentially important cellular regulator of HIV-1, thereby establishing a link between histone metabolism, inflammation, and HIV-1 infection PMID: 27454292
2. showed that inhibiting the SLBP mRNA and protein levels were rescued by epigenetic modifiers suggesting that nickel's effects on SLBP may be mediated via epigenetic mechanisms PMID: 28306745
3. Cyclin F-mediated degradation of SLBP limits H2A.X accumulation and apoptosis upon genotoxic stress in G2 cell cycle checkpoint. PMID: 27773672
4. CRL4(WDR23) is required for efficient histone mRNA 3' end processing to produce mature histone mRNAs for translation. CRL4(WDR23) binds and ubiquitylates SLBP in vitro and in vivo, and this modification activates SLBP function in histone mRNA 3' end processing without affecting its protein levels. PMID: 27203182
5. FEM1 proteins are ancient regulators of Stem-Loop Binding Protein. PMID: 28118078
6. CRL4-DCAF11 mediates the degradation of SLBP at the end of S phase and this degradation is essential for the viability of cells. PMID: 27254819
7. arsenic, a carcinogenic metal, decreases cellular levels of SLBP by inducing its proteasomal degradation and inhibiting SLBP transcription via epigenetic mechanisms PMID: 25266719
8. the S/G2 stable mutant form of SLBP is degraded by proteasome in G1, indicating that indicating that the SLBP degradation in G1 is independent of the previously identified SLBP degradation at S/G2 PMID: 24122909
9. Although the C-terminal tail of dSLBP does not contact the RNA, phosphorylation of the tail promotes SLBP conformations competent for RNA binding and thereby appears to reduce the entropic penalty for the association. PMID: 25002523
10. Alternative splicing allows the synthesis of HBP/SLBP isoforms with different properties that may be important for regulating HBP/SLBP functions during replication stress. PMID: 23941746
11. C-terminal extension of Lsm4 interacts directly with the histone mRNP, contacting both SLBP and 3'hExo. PMID: 24255165
12. Data suggest that oligomerization and SLBP phosphorylation regulate SLBP-SLIP1-histone-mRNA complex formation/disassociation; sequential and ordered assembly is required. PMID: 23286197
13. Using yeast two-hybrid screening, the authors identify CT initiation factor-interacting protein (CTIF) as a protein that binds directly to SLBP. SLBP preferentially associates with the CT complex of histone mRNAs consisting of CBP80/CBP20, but not with the eIF4E/eIF4G (ET) complex, as has been proposed. Rapid degradation of histone mRNA on the inhibition of DNA replication requires association of SLBP with CTIF. PMID: 23234701
14. This paper shows that SLBP is a substrate for the prolyl isomerase Pin1. Pin1, along with PP2A, facilitates dissociation of the SLBP-histone mRNA complex at the end of S-phase, thereby promoting histone mRNA decay and SLBP ubiquitination. PMID: 22907757
15. This paper describes the biophysical characterization of human SLBP and the SLBP-SLIP1 complex. Human SLBP is an intrinsically disordered protein that is phosphorylated at 23 Ser/Thr sites when expressed in a eukaryotic expression system such as baculovirus. Unphosphorylated human SLBP forms a high affinity heterotetramer with SLIP1 and the SLBP-SLIP1 complex is regulated by SLBP phosphorylation. PMID: 23286197
16. the crystal structure of a ternary complex of human SLBP RNA binding domain, human 3'hExo, and a 26-nucleotide stem-loop RNA is reported. PMID: 23329046
17. The nuclear magnetic resonance and kinetic studies presented here provide a framework for understanding how SLBP recognizes histone mRNA and highlight possible structural roles of phosphorylation and proline isomerization in RNA binding proteins PMID: 22439849
18. SLBP is the only cell cycle-regulated factor required for histone pre-mRNA processing PMID: 12588979
19. human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle PMID: 15546920
20. SLBP is imported into the cell nucleus during the cell cycle. PMID: 15829567
21. SLBP is required for efficient DNA replication probably because a decreased ability to assemble chromatin results in a decrease in the rate of DNA replication PMID: 15916543
22. Removal of the phosphoryl group from T230 by either dephosphorylation or mutation results in a 7-fold reduction in the affinity of SLBP for the stem-loop RNA PMID: 16492733
23. replication-dependent histone mRNAs are likely to be the sole target of SLBP PMID: 16931877
24. SLBP is required for efficient histone 3'-UTR end processing. PMID: 16982637
25. Here we show that NELF interacts with the cap binding complex (CBC), a factor that plays important roles in mRNA processing steps, and the two factors together participate in the 3' end processing of histone mRNAs, through association with the SLBP. PMID: 17499042
26. identified five conserved residues in a 15-amino-acid region in the amino-terminal portion of SLBP, each of which is required for translation. PMID: 18025107
27. Study concludes that the increase in cyclin A/Cdk1 activity at the end of S phase triggers degradation of SLBP at S/G(2). PMID: 18490441
28. These results suggest a previously undescribed role for SLBP in histone mRNA export. PMID: 19155325
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

SLBP family
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 10904

OMIM: 602422

KEGG: hsa:7884

STRING: 9606.ENSP00000417686

UniGene: Hs.298345