Recombinant Human Granzyme A (GZMA)

中文名稱：

人GZMA重組蛋白
貨號：

CSB-YP010081HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人GZMA重組蛋白
貨號：

CSB-EP010081HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人GZMA重組蛋白
貨號：

CSB-EP010081HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人GZMA重組蛋白
貨號：

CSB-BP010081HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人GZMA重組蛋白
貨號：

CSB-MP010081HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

GZMA
Uniprot No.：

P12544
別名：

CTL tryptase; CTLA3; Cytolytic t cell and natural killer cell specific trypsin like serine protease; Cytotoxic T lymphocyte associated serine esterase 3; Cytotoxic T lymphocyte proteinase 1; Cytotoxic T-lymphocyte proteinase 1; Fragmentin 1; Fragmentin-1; GRAA_HUMAN; Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3; Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3, Hanukah factor serine protease); Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3); Granzyme A; Granzyme A precursor; Granzyme-1; GZMA; H factor; Hanukah factor serine protease; Hanukkah factor; HF; HFSP; TSP1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

29-262
氨基酸序列

II GGNEVTPHSR PYMVLLSLDR KTICAGALIA KDWVLTAAHC NLNKRSQVIL GAHSITREEP TKQIMLVKKE FPYPCYDPAT REGDLKLLQL MEKAKINKYV TILHLPKKGD DVKPGTMCQV AGWGRTHNSA SWSDTLREVN ITIIDRKVCN DRNHYNFNPV IGMNMVCAGS LRGGRDSCNG DSGSPLLCEG VFRGVTSFGL ENKCGDPRGP GVYILLSKKH LNWIIMTIKG AV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
基因功能參考文獻：
1. GzmA produced by lesional CD8 T cells has the capacity to specifically increase chemokine secretion from inflamed keratinocytes, thereby sustaining the focal inflammation in psoriasis lesions by amplifying a chemotactic inflammatory loop. PMID: 28094457
2. granzyme A in human platelets increases with aging PMID: 29167233
3. GzmA plays an unfavorable role in host defense during pneumococcal pneumonia by a mechanism that does not depend on natural killer cells. PMID: 27343190
4. Carbamate pesticides significantly reduced the intracellular levels of perforin, GrA, GrB, Gr3/K, and GRN in NK-92CI cells. PMID: 25921628
5. Our results suggest that granzyme A could be considered another biomarker of TB, that can be used, other than IFN-gamma, to discriminate between patients with active TB and LTBI subjects PMID: 26051682
6. Delivered into parasite infected cells by granulysin and perforin, granzymes generate superoxide and inactivate oxidative defense enzymes to kill the parasite. PMID: 26752517
7. Taken together, the authors verified that histone H3 is a real substrate for GzmA in vivo in the Raji cells treated by staurosporin. PMID: 26032366
8. Results reveal enhanced intra- and extracellular expression of gzmA and B in patients with pulmonary TB, suggesting that gzms are part of the host response to tuberculosis. PMID: 26156785
9. Levels of GZMA and ITGAE mRNAs in colon tissues can identify patients with UC who are most likely to benefit from etrolizumab; expression levels decrease with etrolizumab administration in biomarker(high) patients. PMID: 26522261
10. GzmA and GzmB expressing cells in the airways and lung parenchyma was higher in subjects who died from asthma PMID: 25745046
11. Data indicate N-terminomics on the human and mouse granzymes A and K by combined fractional diagonal chromatography (COFRADIC). PMID: 25383893
12. We herewith identify GZMA as critical effector molecule of human Treg function for gastrointestinal immune response in an experimental GvHD model. PMID: 25928296
13. The exocytosed granzyme A enters target cells and mediates IL-1beta maturation independently of caspase-1 and without inducing cytotoxicity. PMID: 25437548
14. Serum concentrations of granzyme A in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas. PMID: 24673566
15. functional divergence between human and mouse granzyme A PMID: 24505135
16. Patients with chronic immune thrombocytopenia have elevated serum granzyme A levels. PMID: 22476618
17. A critical and previously unsuspected role is revealed for granzymes A and B in dictating immunogenicity by influencing the mode of tumor cell death. PMID: 21709155
18. The results indicate that GzmA and GzmB are implicated in mechanisms of neurodegeneration in amyotrophic lateral sclerosis. PMID: 21349256
19. investigation of substrate specificity toward Jurkat cell proteins; more than 260 cleavage sites, almost exclusively favoring basic residues at the P1 position, in approximately 200 unique protein substrates, were identified PMID: 20536382
20. Human eosinophils exert TNF-alpha and granzyme A-mediated tumoricidal activity toward colon carcinoma cells. PMID: 21068403
21. HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A. PMID: 11909973
22. results demonstrate discordant expression of granzymes A and B in human lymphocyte subsets and T regulatory cells, which suggests that different granzymes may play unique roles in immune system responses and regulation PMID: 15238416
23. polymorphonuclear leukocytes from mice and humans lack the 3 cytotoxic effector molecules, gzmA, granzyme B, and perforin, generally associated with natural killer and cytotoxic T lymphocytes PMID: 15998831
24. Mouse granzyme B is 30 times less cytotoxic than human granzyme B and does not require Bid for killing but regains cytotoxicity on engineering of its active site cleft. Mouse granzyme A is considerably more cytotoxic than human granzyme A. PMID: 17116752
25. GrA expression is increased in type II pneumocytes of patients with very severe COPD. These results indicate that GrA may be important in the development of COPD. PMID: 17138956
26. GzmK-induced caspase-independent death occurs through Bid-dependent mitochondrial damage that is different from GzmA PMID: 17308307
27. Report activation of the granzymeA/B pathway in children with severe respiratory syncytial virus infection. PMID: 18317234
28. GzmA accesses the mitochondrial matrix to cleave the complex I protein NDUFS3, an iron-sulfur subunit of the NADH:ubiquinone oxidoreductase complex I. PMID: 18485875
29. GZMA causes detachment of alveolar epithelial A549 cells accompanied by interleukin-8 release. PMID: 18776661
30. the granule secretory pathway plays an unexpected role in inflammation, with GzmA acting as an endogenous modulator PMID: 18951048
31. Data suggest that 1, 25(OH)(2) vitamin D(3) suppresses granzyme A probably by down-regulating Th1 cytokine response, and tah vitamin D receptor gene variants might regulate cytotoxic T-cell response by suppression of granzyme A expression in tuberculosis. PMID: 19014932
32. GrK not only constitutes a redundant functional backup mechanism that assists GrA-induced cell death but that it also displays a unique function by cleaving its own specific substrates. PMID: 19059912
33. The N-terminal GzmA cleavage fragment of PARP-1 acts as a PARP-1 dominant negative, binding to DNA and blocking DNA repair. PMID: 19506301
34. Granzyme A is a proinflammatory protease. PMID: 19875524
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亞細胞定位：

[Isoform alpha]: Secreted. Cytoplasmic granule.
蛋白家族：

Peptidase S1 family, Granzyme subfamily
數據庫鏈接：

HGNC: 4708

OMIM: 140050

KEGG: hsa:3001

STRING: 9606.ENSP00000274306

UniGene: Hs.90708