Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), partial

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中文名稱：

人GAPDHS重組蛋白
品名簡稱：

Recombinant Human GAPDHS protein, partial
貨號：

CSB-BP009254HU
說明書：

MSDS Datasheet
規(guī)格：

￥2832
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

GAPDHS
Uniprot No.：

O14556
別名：

G3PT_HUMAN; GAPD2; GAPDH 2 ; GAPDH-2; GAPDH2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase; testis specific; Glyceraldehyde-3-phosphate dehydrogenase; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; testis-specific
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Baculovirus
分子量：

39.1 kDa
表達區(qū)域：

69-408aa
氨基酸序列

MVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
基因功能參考文獻：
1. GAPDS overexpression antagonize high glucose-induced apoptosis by controlling reactive oxygen species accumulation in TM3 cells. PMID: 29626473
2. It is assumed that the deficiency in GAPDS observed in most dysplasia of the fibrous sheath sperm samples is ascribable to a disorder in the regulation of GAPDS expression caused by the mutation in the intron region of GAPDS gene. PMID: 27135296
3. This result implies that GAPDHS participates in specific signal-transduction pathways. PMID: 26660717
4. Structural basis for the NAD binding cooperativity and catalytic characteristics of sperm-specific glyceraldehyde-3-phosphate dehydrogenase PMID: 25936797
5. GAPDS protein was expressed in both Sertoli cells and elongated sperms. The expression of GAPDS gradually increased with age in the epididymis. PMID: 23306140
6. Data indicate that the positive expression of studied genes fertilization rate for GAPDHS positive subset was 66%, ACR - 71%, SPATA22 - 68%, MND1 - 70%, pregnancy rates were 8%, 6%, 18% and 36% respectively. PMID: 23675907
7. the expression of GAPDS in melanoma cells may facilitate glycolysis and prevent the induction of apoptosis. PMID: 23026046
8. The chaperonin TRiC was shown to assist an ATP-dependent refolding of recombinant forms of GAPDS. PMID: 21995946
9. Structure and kinetic characterization of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, GAPDS. PMID: 21269272
10. Human his-GAPDHS expressed in baculovirus-infected insect cells is homotetrameric. PMID: 20828617
11. Data suggest that high stability of the sperm-specific GAPDS is of importance for the efficiency of fertilization. PMID: 20833277
12. We have concluded that a simple nuclear localization of GAPDH does not induce apoptosis, and that MPP+-induced apoptosis is not caused by nuclear translocation of GAPDH. PMID: 16324157
13. Comparative study of the amino acid sequences of mammalian GAPDs revealed conservative cysteine residues (C21, C94, and C150) that are specific for the sperm isoenzyme and absent in the somatic isoenzyme. PMID: 18298375
14. Characterization of the rat GAPDHS and comparison to the human and mouse enzymes. PMID: 16700075
15. GAPD2 enzymatic activity is present in human sperm. GAPD2 is tightly bound to sperm and most enzymatic activity is not released by permeabilization with CHAPS detergent. GAPD2 is restricted in location to the principal piece of the sperm flagellum. PMID: 10714828
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亞細胞定位：

Cytoplasm.
蛋白家族：

Glyceraldehyde-3-phosphate dehydrogenase family
組織特異性：

Testis specific.
數(shù)據(jù)庫鏈接：

HGNC: 24864

OMIM: 609169

KEGG: hsa:26330

STRING: 9606.ENSP00000222286

UniGene: Hs.248017