Recombinant Human Enhancer of filamentation 1 (NEDD9), partial
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中文名稱：人NEDD9重組蛋白
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貨號：CSB-EP622771HU-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：NEDD9
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Uniprot No.：
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別名：Cas like docking; Cas scaffolding protein family member 2; CAS-L; CAS2; CasL; CASL_HUMAN; CASS2; Crk associated substrate related; Crk associated substrate related protein; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ49G10.2; dJ761I2.1 (enhancer of filamentation (HEF1)); dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF 1; HEF1; NEDD-9; Nedd9; Neural precursor cell expressed developmentally down-regulated protein 9; P105; Renal carcinoma antigen NY-REN-12
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種屬：Homo sapiens (Human)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
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靶點詳情
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功能：Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. Required for correct adhesion and migration of T-cells.
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基因功能參考文獻：
- NEDD9, E-cadherin and gamma-catenin proteins have roles in pancreatic ductal adenocarcinoma PMID: 29924959
- Results reveal the role of a phosphorylation-dependent HEF1-Plk1 complex in HEF1 translocation to FAs to induce cell migration. PMID: 29191835
- Overexpression of NEDD9 promotes cell invasion and metastasis in hepatocellular carcinoma. Also, NEDD9 may serve as potential therapeutic target for the treatment of hepatocellular carcinoma. PMID: 28578938
- MiR-125a-5p may serve as a therapeutic agent for lung adenocarcinoma through its major target, NEDD9. PMID: 28714018
- NEDD9 deregulated Smad7 expression to inhibit Smad signaling and binding to the FAK-Src-Crk complex which is proposed that this is the mechanism by which NEDD9 induced greater sphere formation in cancer stem cells properties. PMID: 27974675
- HIP2 regulates mitotic spindle alignment. SHIP2 is expressed in G1 phase, whereas Aurora A kinase is enriched in mitosis. SHIP2 binds Aurora A kinase and the scaffolding protein HEF1 and promotes their basolateral localization at the expense of their luminal expression connected with cilia resorption. PMID: 27926875
- The protein expression level of NEDD9 is higher in esophageal carcinoma tissues than that in adjacent normal tissues. Knockdown of NEDD9 expression can restrain the proliferation, invasion and migration of EC109 cells. PMID: 27916098
- NEDD9 was able to partially overcome the inhibitory effects of miR-203 on colorectal cancer cell colony formation and invasion. PMID: 27771718
- Increased NEDD9 expression is associated with metastatic endometrial and lung cancers. PMID: 26701889
- NEDD9 has an essential role in development as well as in neoplastic cell proliferation, apoptosis, adhesion, migration and invasion. (Review) PMID: 26324022
- The presence of the dna fragment in the NSCLC-N6-L16 cell ChIP assays demonstrated a physical link between NEDD9 and p53. PMID: 26011298
- siRNA suppression of NEDD9 inhibits proliferation and enhances apoptosis in renal cell carcinoma. PMID: 26351211
- Serum NEDD9 was elevated in gastric cancer patients and had an unfavorable prognostic effect. PMID: 26211595
- BCAR3 is an essential interactor and mediator of HEF1-induced migration. PMID: 25817040
- Study uncovers a new role of NEDD9 as a regulator of integrin trafficking through CAV1-dependent endocytosis, and provide new insight into the regulation of migration of breast cancer cells. PMID: 25319010
- miR145 is inversely correlated with NEDD9 expression in pancreatic cancer tissues and restoration of miR145 in Panc1 cells reduced NEDD9 mRNA and protein expression accompanied by inhibition of cell proliferation, invasion and migration. PMID: 25646678
- Results establish a novel role for NEDD9 as a key regulator of Arf6-dependent trafficking of MMP14 and its invasive properties, highlighting the basic mechanism utilized by NEDD9 to support breast cancer cell invasion and metastasis. PMID: 25241893
- discuss emerging evidence supporting involvement of NEDD9 in additional pathological conditions, including stroke and polycystic kidney disease PMID: 25967390
- our findings suggest that NEDD9 acts as an oncogene and promotes GC metastasis via EMT. PMID: 25577245
- The expression of Nedd9 was upregulated in gastric cancer, high expression of Nedd9 significantly correlated with cancer progression and poor prognosis in gastric cancer. PMID: 24906654
- Knockdown of the NEDD9 gene results in suppression of lung adenocarcinoma cell proliferation, migration, invasion and cell growth in vitro and in vivo PMID: 25051398
- NEDD9 expression in breast cancer cells enhances the expression of chondroitin sulfate glycosaminoglycans, particularly the CS-E subunit that enhances growth. PMID: 25445787
- NEDD9 binds to and regulates acetylation of CTTN in an Aurora A kinase (AURKA)/HDAC6-dependent manner. PMID: 24574519
- Colorectal adenocarcinoma patients with a higher NEDD9 expression had a significantly shorter overall survival. PMID: 24966970
- Data reveal that NEDD9 can regulate 3D migration speed independent of the Rac1 morphology switch. PMID: 24337070
- NEDD9 may be used as a biomarker in the clinical setting to predict the prognosis of gastric cancer patients. PMID: 24664584
- Obtained data revealed remarkable increase in the expression levels of GHR and NEDD9 proteins in both epithelial and stromal components of axillary lymph node metastases in comparison with those of non-metastatic tumours. PMID: 24676793
- NEDD9 overexpression correlates with the progression and prognosis in gastric carcinoma. PMID: 24469954
- Depletion of NEDD9 impaired invasion of cancer cells through inactivation of membrane-bound matrix metalloproteinase MMP14 by excess TIMP2 on the cell surface. PMID: 24202705
- NEDD9 promotes migration and invasion in cervical carcinoma cells, probably via a positive feedback loop of tyrosine phosphorylation between NEDD9 and FAK or SRC. PMID: 24058594
- NEDD9 regulates epithelial-mesenchmal transition and cell invasion in prostate cancer cells induced by TGF-beta. PMID: 24728978
- Data indicate that NEDD9 knockdown significantly inhibits migration, invasion and metastasis of lung cancer cells in vitro and in vivo. PMID: 24174333
- NEDD9 overexpression is an independent factor of poor prognosis and may serve as a potential biomarker in epithelial ovarian cancer PMID: 24439227
- The mean overall survival of NSCLC patients overexpressing NEDD9 (39.10 +/- 6.49 months) was markedly shorter. PMID: 23086683
- The findings indicate that HEF1 promotes EMT and bone invasion in prostate cancer by directly targeted by miR-145, and miR-145 suppresses EMT and invasion, at least in part, through repressing HEF1. PMID: 23355420
- NEDD9 is upregulated in lung adenocarcinoma, and overexpression of NEDD9 protein has been strongly correlated with staging and differentiation grade and tumor size in lung adenocarcinoma. PMID: 22447485
- High NEDD9 expression is associated with pancreatic ductal adenocarcinoma. PMID: 23247867
- Overexpression of NEDD9 promoted lung metastasis of an lung cancer cell line in transgenic mice. PMID: 23037767
- Results demonstrate the critical role of miR-145 and NEDD9 in regulating glioblastoma invasion and suggest a potential role of NEDD9 as a biomarker for glioma progression. PMID: 22869051
- In clinical specimens, elevated expression of NEDD9 was associated with malignant progression and metastasis.CRTC1-NEDD9 signaling axis mediates lung cancer progression caused by LKB1 loss. PMID: 23074285
- signalling through increased NEDD9 levels requires integrin beta3 signalling, which leads to elevated phosphorylation of integrin beta3 PMID: 22328516
- The GG genotype of NEDD9 rs760678 polymorphism would be a protective factor for AD in Caucasians but not in Asians.[meta-analysis] PMID: 22963925
- Abl family kinases modulate T cell-mediated inflammation and chemokine-induced migration through the adaptor HEF1 and the GTPase Rap1. PMID: 22810897
- HEF1 may have a role in carcinogenesis of lung cancer; miRNA be a therapeutic target PMID: 22732573
- Polymorphism, rs760678, within NEDD9 gene is association with the risk of AD and cognitive performance in Chinese older persons PMID: 21399483
- Data reveal that the tumour suppressor PP2A may act via S369 to regulated NEDD9-mediated cell spreading. PMID: 22061964
- Cas proteins do not affect E-cadherin transcription, but rather, BCAR1 and NEDD9 signal through SRC to promote E-cadherin removal from the cell membrane and lysosomal degradation. PMID: 21765937
- Data suggest that HEF1 may represent an attractive candidate for drug targeting in CRC. PMID: 21317929
- NEDD9 polymorphism has a possible role in changing the genetic susceptibility to late-onset Alzheimer's disease in a Han Chinese population. PMID: 21059344
- HEF1 is required for VEGF-mediated Head and Neck Squamous Cell Carcinoma cell migration and invasion. PMID: 20498643
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亞細胞定位：Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.; [Enhancer of filamentation 1 p55]: Cytoplasm, cytoskeleton, spindle.
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蛋白家族：CAS family
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組織特異性：Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.
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