E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
基因功能參考文獻(xiàn):
In this young Chinese population, CDH13 rs4783244 represents a key locus for cardiac structure, and confers stronger cardio-protection in longer sleep duration when contrasted with short sleep duration PMID: 29903569
Polymorphism in CDH13 is associated with nephropathy in subjects with type 1 diabetes. PMID: 28499019
The single nucleotide polymorphisms (SNPs) rs12596316AG genotype of the T-cadherin (CDH13) gene is associated with the susceptibility to metabolic syndrome (MS) among ethnic Han Chinese. PMID: 29419874
the data suggests that the CDH13 T > A (rs11646213) polymorphism is associated with decreased risk of developing hypertension in the Mexican population PMID: 27682011
nine ADHD candidate single nucleotide polymorphisms (SNPs) in seven genes were tested for association with PD in 5333 cases and 12,019 healthy controls. No significant association was observed. PMID: 28176268
CDH13 genetic polymorphisms are associated with adiponectin levels and ischemic stroke. PMID: 28245897
Study evaluated the associations between 6 SNPs in CDH13 and type 2 diabetes mellitus (T2DM) in a Han Chinese population. Results showed that the rs12596316 AG genotype was a risk genotype for the development of T2DM in the overdominant inheritance model; rs11646213, rs3865188, rs12444338, rs12051272, and rs7195409 had no observed associations with T2DM in terms of alleles, genotypes, and the various inheritance models. PMID: 28562572
The methylation status of CDH13 promoter was strongly associated with breast cancer risk. However, CDH13 promoter methylation was not significantly related to the OS and DFS of breast cancer and may have limited prognostic value for breast cancer patients PMID: 27153114
Studied the associations between genetic variants of CDH13 and type 2 diabetes (T2D), and its related parameters, in a Caucasian population. PMID: 27289142
Compared with adjacent normal tissues, the methylation frequencies of WIF-1, RASSF1A, and CDH13 genes were significantly higher but the mRNA levels of these 3 genes were significantly lower in EC tissues. The survival rates of patients with WIF-1, RASSF1A, and CDH13 methylations were significantly lower than those of patients without methylation PMID: 27506957
higher promoter methylation in colorectal cancer than in premalignant, normal, adjacent tissues; highest promoter methylation in poorly differentiated colorectal cancer PMID: 28121942
CDH13 genetic variants determine Chinese individuals' susceptibility to chronic obstructive pulmonary disease (COPD) and thus are efficient genetic biomarkers for early detection of COPD. PMID: 26806298
Strong Functional Association of adipor2 and cdh13 with adipoq PMID: 25388841
CDH13 Polymorphisms are Associated with Adiponectin Levels and Metabolic Syndrome Traits Independently of Visceral Fat Mass. PMID: 26423718
CDH13 locus variants and adiponectin levels are associated with circulating levels of cellular adhesion molecules and adiposity status in a differential manner that interacts with sex PMID: 26600672
This study finding significant excess of rare nonsynonymous variants exclusive to European Americans smokers in CHD13. PMID: 25450229
Data demonstrate for the first time that SHP1 methylation has high specificity for diagnosis of endometrial carcinoma, while CDH13 promoter methylation plays a role in the earlier stage. PMID: 26597461
The present study identified a new genetic factor for CRC risk and an interaction between CDH13 and APN in CRC risk. These genetic factors may be useful for predicting CRC risk. PMID: 26362652
The downregulation of T-cadherin may contribute to gastric cancer progression, representing a useful biomarker for predicting the biological behavior and prognosis of gastric cancer. PMID: 25847144
Finnish prisoners, revealed that a monoamine oxidase A (MAOA) low-activity genotype (contributing to low dopamine turnover rate) as well as the CDH13 gene (coding for neuronal membrane adhesion protein) are associated with extremely violent behavior PMID: 25349169
The genetic polymorphisms at the CDH13 locus independently affect the adiponectin levels, whereas the adiponectin levels exhibit a suppressive effect on the association between CDH13 locus variants and various metabolic phenotypes and metabolic syndrome PMID: 25875811
The CDH13 rs11150556 CC genotype was associated with more hyperactive/impulsive symptoms in attention/deficit hyperactivity disorder. PMID: 25739828
CDH13 promoter methylation is associated with lung cancer. PMID: 25735345
Aberrant promoter methylation of the cadherin 13 gene in serum is associated with prostate cancer. PMID: 25015764
Our results suggested that negative T-cadherin expression has a worse prognosis in patients with axillary lymph node-positive breast cancer. PMID: 25677746
down-regulation of adiponectin receptors (AdipoR1, R2, and T-cadherin) in osteoarthritic chondrocytes PMID: 24888493
CDH13 DNA is methylated in only ten percent of cervical cancer patients. PMID: 22942707
High frequency of CDH13 hypermethylation is associated with melanoma brain metastases. PMID: 24968695
CDH13 methylation is a frequent event in non-muscle invasive bladder cancer. PMID: 25196672
Several SNPs in the CDH13 promoter region are significantly associated with the level of DNA methylation at nearby CpG sites. PMID: 25543204
T-cadherin regulates prostate cancer cell behavior by tuning the balance in EGFR/IGF-1R activity and enhancing the impact of IGF-1R PMID: 25381040
High methylation level of CDH13 gene promoter region is associated with low drug sensitivity of non-small cell lung cancer. PMID: 24998565
T-cadherin exhibited a state of insulin insensitivity as evidenced by attenuation of the ability of insulin to stimulate Akt/mTOR axis signaling, phosphorylation of MLC20 and MYPT1 PMID: 24815187
Adiponectin/T-cadherin and apelin/APJ expression patterns were found to be inversely associated with human aortic and coronary atherosclerosis. PMID: 24675084
CDH13 genotype may be a factor that affects not only the plasma level of HMWA but also the prognostic significance of HMWA. PMID: 24041676
A non significant trend for higher levels of total adiponectin was observed in adult attention deficit hyperactivity disorder patients carrying CDH13 missense mutations compared to patients with wild type CDH13. PMID: 24559850
The SNPs in ADP-ribosylation factor-like protein 15 (ARL15) and the T-cadherin (CDH13) genes did not exhibit significant association with individual metabolic traits in the T2DM and NDM groups. PMID: 24688318
The SNPs most strongly associated in the single-marker analysis of the combined Danish samples were rs4757144 in ARNTL (P=3.78 x 10-6) and rs8057927 in CDH13. PMID: 23358160
High CDH-13 expression is associated with melanoma. PMID: 23625515
The results provide evidence that CDH13 variants are associated with metabolic traits and carotid atherosclerosis in Koreans. PMID: 24142632
genome-wide association study in populations in Minnesota and Utah: Data suggest that SNPs on chromosome 12 and in CDH13 (H-cadherin) are associated with baseline circulating levels of adiponectin (and response of adiponectin levels to fenofibrate). PMID: 23149075
high expressionsin uterine leiomyoma, associated with color Doppler flow imaging PMID: 23463326
the frequency of coding CDH13 variants in adult attention deficit/hyperactivity disorder PMID: 23936508
Cadherin 13 might play a role in the protoporphyrin (Pp)IX accumulation pathway and act as a negative regulator of 5-ALA-induced fluorescence in glioma cells. PMID: 24010971
CDH13 variants strongly influence plasma total and high molecular weight adiponectin levels in East Asian populations but appear to alter adiponectin sensitivity, resulting in better metabolic health than expected based on circulating adiponectin levels. PMID: 24009259
T-cadherin translocation to cell-cell contacts is sensitive to the activity status of EGFR, requires lipid raft domain integrity and actin filament polymerization, and crucial intracellular signalling mediators include Rac1 and p38MAPK. PMID: 23411345
Downregulated expression of CDH13 is associated with increased invasion of bladder transitional cell carcinoma PMID: 23235385
Data indicate that T-cadherin-silencing promotes experimental metastasis of squamous cell carcinoma (SCC). PMID: 23369463
The present study investigated transcriptional regulation of CDH13 in melanoma. Found an inverse correlation between BRN2 and T-cadherin protein and transcript expression in melanoma. PMID: 23069940
This study identifying loci for aADHD and led to the identification of CHD13 as novel genes associated with ADHD across the lifespan. PMID: 22105624
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亞細(xì)胞定位:
Cell membrane; Lipid-anchor, GPI-anchor.
組織特異性:
Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord.