Recombinant Human Beta,beta-carotene 15,15'-monooxygenase (BCMO1)

中文名稱：

Recombinant Human Beta,beta-carotene 15,15'-monooxygenase(BCMO1)
貨號：

CSB-YP864015HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human Beta,beta-carotene 15,15'-monooxygenase(BCMO1)
貨號：

CSB-EP864015HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human Beta,beta-carotene 15,15'-monooxygenase(BCMO1)
貨號：

CSB-EP864015HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human Beta,beta-carotene 15,15'-monooxygenase(BCMO1)
貨號：

CSB-BP864015HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human Beta,beta-carotene 15,15'-monooxygenase(BCMO1)
貨號：

CSB-MP864015HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

BCO1
Uniprot No.：

Q9HAY6
別名：

BCDO; BCDO1; BCDO1_HUMAN; BCMO; Bcmo1; BCO; BCO1; Beta,beta-carotene 15,15'-monooxygenase; beta-carotene 15,15'-monooxygenase 1; Beta-carotene dioxygenase 1; FLJ10730
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-547
氨基酸序列

MDIIFGRNRK EQLEPVRAKV TGKIPAWLQG TLLRNGPGMH TVGESRYNHW FDGLALLHSF TIRDGEVYYR SKYLRSDTYN TNIEANRIVV SEFGTMAYPD PCKNIFSKAF SYLSHTIPDF TDNCLINIMK CGEDFYATSE TNYIRKINPQ TLETLEKVDY RKYVAVNLAT SHPHYDEAGN VLNMGTSIVE KGKTKYVIFK IPATVPEGKK QGKSPWKHTE VFCSIPSRSL LSPSYYHSFG VTENYVIFLE QPFRLDILKM ATAYIRRMSW ASCLAFHREE KTYIHIIDQR TRQPVQTKFY TDAMVVFHHV NAYEEDGCIV FDVIAYEDNS LYQLFYLANL NQDFKENSRL TSVPTLRRFA VPLHVDKNAE VGTNLIKVAS TTATALKEED GQVYCQPEFL YEGLELPRVN YAHNGKQYRY VFATGVQWSP IPTKIIKYDI LTKSSLKWRE DDCWPAEPLF VPAPGAKDED DGVILSAIVS TDPQKLPFLL ILDAKSFTEL ARASVDVDMH MDLHGLFITD MDWDTKKQAA SEEQRDRASD CHGAPLT
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Symmetrically cleaves beta-carotene into two molecules of retinal using a dioxygenase mechanism.
基因功能參考文獻：
1. We did not observe associations of BCMO1 variants and lung cancer. PMID: 29673335
2. Study reports a molecular mechanism by which glucocorticoid-induced PPARalpha expression negatively affects the activity of PPARgamma and downregulates BCO1 gene expression. Results explicate novel aspects of local glucocorticoid:retinoid interactions that may contribute to alveolar tissue remodeling in chronic lung diseases that affect children and, possibly, adults. PMID: 28732066
3. study revealed circulating beta-carotene levels were significantly higher in rs6564851 GG homozygotes; daily intake of beta-cryptoxanthin was positively associated with circulating beta-carotene levels in female GG homozygotes of rs6564851 and the daily intake of alpha- and beta-carotenes, and beta-cryptoxanthin was significantly lower in female rs6564851 T allele carries than in female GG homozygotes PMID: 28005968
4. Data suggest that beta-cryptoxanthin is a poorer substrate for BCMO1 than is beta-carotene; however, the comparatively high bioavailability of beta-cryptoxanthin from foods makes beta-cryptoxanthin-rich fruits good sources of vitamin A. [REVIEW] PMID: 25270992
5. This study indicates that the competitive actions of HNF-1alpha and HNF-4alpha on their overlapping binding sites in the human BCMO1 gene promoter oppositely regulate BCMO1 gene expression in the human small intestine. PMID: 25445224
6. Specific BCMO1 SNPs should be determined when assessing the effects of carotenoid supplementation on macular pigment and that their expression may be influenced by retinal disease. PMID: 24586510
7. Together with the data from (18)O-retinal-H2(16)O and (16)O-retinal-H2(18)O incubations to account for nonenzymatic oxygen exchange, our results show that BCO1 incorporates only oxygen from O2 into retinal. Thus, BCO1 is a dioxygenase. PMID: 24668807
8. Incubation of beta-carotene and recombinant human BCMO1 in either H2(18)O-(16)O2 or H2(16)O-(18)O2 medium yields two retinal products both of which contain oxygen atoms originating solely from O2 gas. The results show that BCMO1 is a dioxygenase and not a monooxygenase as previously thought. PMID: 24668807
9. Substrate specificity of purified recombinant human beta-carotene 15,15'-oxygenase (BCO1). PMID: 24187135
10. BCMO1 SNP had a statistically significant association with HDL plasma levels. PMID: 23656756
11. Individual responsiveness to dietary carotenoids was associated with genetic variants of the carotenoid metabolizing enzyme beta-carotene 15,15'-monooxygenase 1. PMID: 23517913
12. a stepwise cleavage by BCO2 and BCO1 with APO10ol as an intermediate could provide a mechanism to tailor asymmetric carotenoids such as beta-cryptoxanthin for vitamin A production. PMID: 24106281
13. Purified BCMO1 is a monomeric enzymatically active soluble protein that does not require cofactors and displays a turnover rate of about 8 molecules of beta,beta-carotene per second. PMID: 23727499
14. CMO1 is expressed in human alveolar epithelial (A549) cells and converts beta-carotene into retinal and biologically active retinoic acids. PMID: 24071514
15. Inhibition of BCMO1 expression is associated with increased invasiveness of colon cancer cells and increased expression of MMP7 and MMP28. beta-Carotene can upregulate BCMO1 and reverse these effects. PMID: 23803888
16. genetic association study in US women of European descent: Data suggest that 2 SNP in BCMO1 (rs4889286; rs12934922) are associated with plasma carotenoid level (i.e., alpha-carotene, beta-carotene, lutein, zeaxanthin). (data from Nurses Health Study) PMID: 23134893
17. investigated the effects of 4 identified SNP 5' upstream from the BCMO1 gene on beta-carotene conversion efficiency; found that three of the four intronic SNPs reduced the catalytic activity of BCMO1 in female volunteers PMID: 22113863
18. These results suggest that BCMO1 and CD36 are implicated in plasma and retina concentrations of lutein and that genetic variants in these genes can modulate blood and retina concentrations of lutein. PMID: 21091228
19. non-synonymous single nucleotide polymorphisms in the BCMO1 gene have been discovered causing observably reduced BCMO1 activity PMID: 20599666
20. Hydrophobic mutations (K108F and K108L) at this position substantially decreased the affinity of the enzyme for substrates with ionone rings at both ends, such as alpha-carotene, beta-carotene, and beta-cryptoxanthine. PMID: 20221844
21. Study demonstrates that human intestinal cell BCMO1 expression is dependent on the functional cooperation between peroxisome proliferator-activated receptor-gamma and myocyte enhancer factor 2 isoforms. PMID: 16504037
22. Haploinsufficiency of the CMO1 enzyme caused hypercarotenemia and hypovitaminosis A in individuals consuming a carotenoid-containing and vitamin A-deficient diet. PMID: 17951468
23. T3 is an important hormone for the regulation of vitamin A and beta-carotene metabolism-related gene expression in human small intestinal cells. PMID: 18282583
24. Two common single nucleotide polymorphisms in the gene encoding BCOM1 alter beta-carotene metabolism in female volunteers. PMID: 19103647
25. Identification of novel common variants associated with circulating carotenoid levels and known lipid variants associated with alpha-tocopherol levels in variations of this enzyme. PMID: 19185284
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相關疾病：

Hypercarotenemia and vitamin A deficiency, autosomal dominant (ADHVAD)
蛋白家族：

Carotenoid oxygenase family
組織特異性：

Highly expressed in retinal pigment epithelium. Also expressed in kidney, testis, liver, brain, small intestine and colon.
數據庫鏈接：

HGNC: 13815

OMIM: 115300

KEGG: hsa:53630

STRING: 9606.ENSP00000258168

UniGene: Hs.212172