Recombinant Human Ammonium transporter Rh type C (RHCG), partial

中文名稱：

人RHCG重組蛋白
貨號(hào)：

CSB-YP880059HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人RHCG重組蛋白
貨號(hào)：

CSB-EP880059HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人RHCG重組蛋白
貨號(hào)：

CSB-EP880059HU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人RHCG重組蛋白
貨號(hào)：

CSB-BP880059HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人RHCG重組蛋白
貨號(hào)：

CSB-MP880059HU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

RHCG
Uniprot No.：

Q9UBD6
別名：

Ammonium transporter Rh type C; C15orf6; CDRC2; chromosome 15 open reading frame 6; PDRC2; Rh family type C glycoprotein; Rh glycoprotein kidney; Rh type C glycoprotein; rhcg; RHCG_HUMAN; Rhesus blood group family type C glycoprotein; Rhesus blood group; C glycoprotein; RHGK; SLC42A3; Tumor-related protein DRC2
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.
基因功能參考文獻(xiàn)：
1. RhCG and H+ATPases are located within the same cellular protein complex in the kidney and this interaction is required for maximal urinary acidification by H+-ATPases, a prerequisite for efficient NH3 secretion and urine excretion of NH4+. PMID: 29054531
2. RHCG was down-regulated in cervical cancers compared to that in normal cervical tissues, and further decreased in cervical cancer cell lines. Functionally, RHCG overexpression reduced cervical cancer cell proliferation and migration, as evidenced by the decreased transforming growth factor (TGF)-beta1, matrix metalloproteinase (MMP)-2 and MMP-9 expressions in cancer cells. PMID: 29852177
3. The results support the notion that RHCG is a novel tumor suppressor gene that plays an important role in the development and progression of ESCC. PMID: 29290801
4. The molecular mechanism of NH4(+) recruitment and NH3 transport by RHCG is described. PMID: 26190573
5. Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) are identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion. PMID: 24647713
6. RhAG, RhBG and RhCG-exhibit significant permeability to NH3 and show for the first time that RhBG and RhCG can conduct CO2. PMID: 24077989
7. Amt and Rh proteins are not functionally equivalent and that permeation takes place according to two distinct mechanisms. PMID: 24021113
8. X-ray crystallographic structure of human RhCG, determined at 2.1 A resolution, reveals the mechanism of ammonia transport. PMID: 20457942
9. major finding of this study is that RhCG protein is active as an NH(3) channel and that this function does not require any protein partner PMID: 20126667
10. RhBG and RhCG facilitate ammonium movement across the plasma membrane; these results favor the hypothesis that these Rh glycoproteins, together with their homolog RhAG, constitute a family of NH3 channels in mammalian cells. PMID: 15929723
11. RhCG expression enhanced the ammonium-induced initial alkalinization (related to NH3 influx)& secondary acidification (related to NH4+ influx). Sub-millimolar NH4+ concentrations induced inward currents in voltage-clamped RhCG-expressing cells. PMID: 16564724
12. Review. Mutations at positions F74, V137 and F235 resulted in a severe reduction of NH3 transport. PMID: 16580862
13. This study concludes that under normal conditions, RhCG is the major putative ammonia transporter expressed in the human kidney and RhBG is not expressed at detectable levels. PMID: 19357182
14. Rhbg and Rhcg may contribute to bronchial epithelial cell ammonia metabolism and suggest that they do not contribute to pulmonary carbon dioxide transport. PMID: 19429772
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亞細(xì)胞定位：

Apical cell membrane; Multi-pass membrane protein.
蛋白家族：

Ammonium transporter (TC 2.A.49) family, Rh subfamily
組織特異性：

Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). According to PubMed:11062476, specifically expressed in kidney.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 18140

OMIM: 605381

KEGG: hsa:51458

STRING: 9606.ENSP00000268122

UniGene: Hs.459284