Recombinant Human Alanine aminotransferase 1 (GPT)

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中文名稱：

人GPT重組蛋白
品名簡稱：

Recombinant Human GPT protein
貨號：

CSB-EP009864HU
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

GPT
Uniprot No.：

P24298
別名：

AAT1; Alanine aminotransferase 1; Alanine aminotransferase; ALAT1_HUMAN; ALT1; Glutamate pyruvate transaminase 1; Glutamic alanine transaminase 1; Glutamic pyruvate transaminase (alanine aminotransferase); Glutamic pyruvic transaminase 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT 1; gpt; GPT1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

58.5kDa
表達區(qū)域：

2-496aa
氨基酸序列

ASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The DNA coding sequence translated into the human GPT protein sequence (2-496aa) was fused with the N-terminal 6xHis tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human GPT protein with N-terminal 6xHis tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 55 kDa protein band was observed on the gel.
GPT is a gene providing instructions for making a protein named alanine aminotransferase 1 (abbreviated GPT) in human and belongs to class-I pyridoxal-phosphate-dependent aminotransferase family or alanine aminotransferase subfamily. This protein is mainly expressed in liver, kidney, heart, and skeletal muscles, and expressed at moderate levels in the adipose tissue. This protein is an enzyme catalyzing the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It is involved in cellular nitrogen metabolism and also in in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
基因功能參考文獻：
1. a relationship between NAFLD with high ALT and lower BMD in the general U.S. population PMID: 29897928
2. The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
3. In men with hepatic steatosis, we found that elevated serum ALT levels were associated with lower serum SHBG levels. PMID: 28540984
4. Our results using Mendelian randomization suggest that ALT reduces the risk of ischaemic heart disease (IHD), probably through reducing triglyceride levels. PMID: 28007909
5. Measurements of serum alpha-fetoprotein (AFP) levels could be helpful in detecting progression or recurrence in treated patients with hepatitis B virus (HBV) - hepatocellular carcinoma (HCC) and normal alanine aminotransferase (ALT). PMID: 27304617
6. Baseline alanine transaminase (ALT) and a hepatitis B surface antigen (HBsAg) above 1000 IU/ml were independent predictive factors for ALT elevation. PMID: 27935953
7. High serum alanine aminotransferase expression is associated with metabolic syndrome. PMID: 27390880
8. The data of this study confirmed the genetic heterogeneity of childhood-onset pure HSP, with SPG4/SPAST and SPG3A/ATL1 being the most frequent forms. PMID: 27260292
9. Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
10. Liver fat content and serum ALT were inversely correlated with bone mineral density in middle-aged and elderly men. PMID: 26791408
11. Age, female gender, and elevated ALT can help identifying cases with biliary etiology of acute pancreatitis. PMID: 26664483
12. IBS [irritable bowel syndrome ] may be an important condition in certain patients with elevated ALT levels and MS [ metabolic syndrome ]. PMID: 26632395
13. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GPT1 rises due to up-regulation of hepatic expression of GPT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
14. Elevated aminotransferase levels, even within the normal range, were significantly associated with increased risk of all external-cause mortalities, including suicide, and injury. PMID: 26446640
15. There are significant relationships of elevated ALT levels with cardiometabolic risk factors (obesity, hypertension, hypercholesteremia, hyperuricemia, hypertriglyceridemia) and several sex-related differences in rural Chinese. PMID: 26160405
16. ALT was significantly associated with elevated levels of triacylglycerols, hyperglycemia, and obesity. In patients with metabolic syndrome, one must consider liver involvement if the patient has ALT levels in the upper third of the reference range. PMID: 25874513
17. Peginterferon alfa-2a is associated with elevations in alanine aminotransferase at the end of treatment in chronic hepatitis C patients with sustained virologic response. PMID: 24937007
18. A prospective association between ALT levels and metabolic syndrome highlights the value of ALT levels, even mild ALT elevations within the normal range, as a predictor of the MetS risk in male schizophrenia patients. PMID: 24819732
19. Serum glutamate pyruvate transaminase levels were elevated postoperatively in recipients of an orthotopic liver transplant from a brain-dead donor receiving extracorporeal membrane oxygenation. PMID: 25543901
20. Statistically significant differences were observed in the mean values of GPT in type 1 diabetics compared to type 2 and control groups PMID: 24849491
21. Alanine aminotransferase showed associations with BMI, glycaemia, lipids, and creatinine. The relationship between alanine aminotransferase and age was best expressed by polynomial regression. PMID: 24953204
22. Serum alanine aminotransferase has limited predictive value for liver disease in chronic hepatitis C PMID: 25219834
23. Role of insulin resistance and adipocytokines on serum alanine aminotransferase in obese patients with type 2 diabetes mellitus PMID: 23884827
24. In nonalcoholic fatty liver disease, adipose tissue insulin resistance (but not hepatic insulin resistance) and liver triglyceride content are major factors in the elevation of plasma aminotransferase levels. PMID: 25145475
25. elevated ALT is common in the Korean population; it is associated with metabolic disorders (obesity, insulin resistance, or metabolic syndrome) in the majority of patients PMID: 24275711
26. ALT and visceral adipose tissue affect metabolic risk factors in a middle-aged Japanese population PMID: 24500143
27. PNPLA3 I148M polymorphism is associated with elevated alanine transaminase levels in Mexican indigenous and Mestizo populations. PMID: 24691744
28. Among patients with chronic HCV infection, daily consumption of filtered coffee may have a beneficial effect on the stabilization of ALT levels. PMID: 24349501
29. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
30. Elevated ALT is associated with developing depressive symptoms, suggesting that non-alcoholic fatty liver disease may represent an independent modifiable risk factor for depression. PMID: 23522007
31. fibrosis is not rare in Chinese CHB patients with persistent normal alanine aminotransferase, especially HBeAg-positive patients over 30 years old PMID: 24205292
32. Elevated serum ALT levels are prevalent among elderly agricultural and fishing population workers in Taiwan. PMID: 24158044
33. Elevated serum alanine aminotransferase level was independently associated with an increased risk of intracerebral hemorrhage in East Asian men. PMID: 23880909
34. Normal to near-normal serum aminotransferase levels are associated with metabolic disorders. PMID: 23794308
35. Data from cross-sectional study in children/adolescents in Republic of Korea suggest normal ALT/GPT serum reference values; ALT/GPT serum level appears effective as biomarker in predicting cardiovascular/metabolic syndrome risk in population studied. PMID: 23403445
36. Among HIV/HCV-coinfected persons, risky drinking was associated with a higher adjusted mean AST (62.2 vs. 51.4 U/L; adjusted ratio of means 1.2, 95% CI [1.07, 1.37], p = .003) and ALT (51.3 vs. 41.6 U/L; compared with non-risky drinking PMID: 23384374
37. Nonalcoholic fatty liver disease and metabolic syndrome were associated with ALT levels in a male population from southern China. PMID: 22975640
38. ALT[Alanine transaminase] may be a marker of an underlying etiology relating to the paradoxical associations of diabetes and IHD at a population level. PMID: 22954960
39. An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold. PMID: 22389715
40. In a cross-sectional analysis of Taiwanese industrial employees, elevated ALT associated with MS, but in subjects who did not meet MS criteria, elevated ALT by itself did not associate with IFG/T2DM. PMID: 21715038
41. Patients with HLA-DQA1 and DRB1 genotypes have higher alanine aminotransferase elevation, whereas HLA-negative cases have a higher incidence of Gilbert's syndrome. PMID: 22357454
42. In a cross-sectional study, Chinese adolescents and young adults with more metabolic syndrome components were more likely to suffer from elevated ALT PMID: 22177542
43. alanine aminotransferase levels largely within the normal range, there was an inverse association between ALT and all-cause mortality and cardiovascular events PMID: 22158663
44. the COMT polymorphism affects serum ALT activity to maintain liver function PMID: 22293393
45. The magnitude of the association of PNPLA3 with liver enzymes is driven by the size of abdominal fat. PMID: 22140488
46. discriminates persons infected with hepatitis C virus from those at low risk of liver disease, but would be considered elevated in a large proportion of the U.S. population PMID: 21987480
47. An hepatitis B virus DNA level of >/=5 log10 copies/mL and an alanine aminotransferase (ALT) level of >20 to PMID: 21692932
48. The prevalence of metabolic syndrome (MS) and risk factors for and association with elevated alanine aminotransferase (ALT) as markers of hepatic injury in a large Hispanic health disparity cohort with high rates of obesity, was studied. PMID: 21720553
49. Subjects with metabolic syndrome had significantly higher plasma levels of alanine aminotransferase. PMID: 21040936
50. Nrmal levels of ALT are associated with metabolic and androgenic phenotypes, i women of reproductive age. PMID: 20961180
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亞細胞定位：

Cytoplasm.
蛋白家族：

Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
組織特異性：

Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
數據庫鏈接：

HGNC: 4552

OMIM: 138200

KEGG: hsa:2875

STRING: 9606.ENSP00000378408

UniGene: Hs.103502