Recombinant Hepatitis GB virus B Genome polyprotein, partial

中文名稱：

Recombinant Hepatitis GB virus B Genome polyprotein ,partial
貨號：

CSB-YP727942HCAR
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Hepatitis GB virus B Genome polyprotein ,partial
貨號：

CSB-EP727942HCAR
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Hepatitis GB virus B Genome polyprotein ,partial
貨號：

CSB-EP727942HCAR-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Hepatitis GB virus B Genome polyprotein ,partial
貨號：

CSB-BP727942HCAR
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Hepatitis GB virus B Genome polyprotein ,partial
貨號：

CSB-MP727942HCAR
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

N/A
Uniprot No.：

Q69422
別名：

Genome polyprotein [Cleaved into: Core protein; Envelope glycoprotein E1; Envelope glycoprotein E2; NS1); p13; p6; p7; Protease NS2-3; EC 3.4.22.-); Serine protease NS3; EC 3.4.21.98; EC 3.6.1.15; EC 3.6.4.13; Hepacivirin; NS3P); Non-structural protein 4A; NS4A); Non-structural protein 4B; NS4B); Non-structural protein 5A; NS5A); RNA-directed RNA polymerase; EC 2.7.7.48; NS5B)]
種屬：

Hepatitis GB virus B (GBV-B) (GB virus B)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Packages viral RNA to form a viral nucleocapsid, and promotes virion budding (Probable). Participates in the viral particle production as a result of its interaction with the non-structural protein 5A. Binds RNA and may function as a RNA chaperone to induce the RNA structural rearrangements taking place during virus replication. Modulates viral translation initiation by interacting with viral IRES and 40S ribosomal subunit. Probably affects various cell signaling pathways, host immunity and lipid metabolism (Probable).; Forms a heterodimer with envelope glycoprotein E2, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. Fusion with the host cell is most likely mediated by both E1 and E2, through conformational rearrangements of the heterodimer required for fusion rather than a classical class II fusion mechanism.; Forms a heterodimer with envelope glycoprotein E1, which mediates virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. Fusion with the host cell is most likely mediated by both E1 and E2, through conformational rearrangements of the heterodimer required for fusion rather than a classical class II fusion mechanism.; May function as a multimeric ion channel protein (viroporin).; Cysteine protease required for the proteolytic auto-cleavage between the non-structural proteins NS2 and NS3. The N-terminus of NS3 is required for the function of NS2 protease (active region NS2-3). Promotes the initiation of viral particle assembly by mediating the interaction between structural and non-structural proteins.; Displays three enzymatic activities: serine protease with a chymotrypsin-like fold, NTPase and RNA helicase. NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. The NS3/NS4A complex prevents phosphorylation of host IRF3, thus preventing the establishment of dsRNA induced antiviral state. NS3 RNA helicase binds to RNA and unwinds both dsDNA and dsRNA in the 3' to 5' direction, and likely resolves RNA complicated stable secondary structures in the template strand. Cleaves host MAVS/CARDIF thereby preventing the establishment of an antiviral state.; Induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex. NS4B self-interaction contributes to its function in membranous web formation.; Phosphorylated protein that is indispensable for viral replication and assembly.; RNA-dependent RNA polymerase that performs primer-template recognition and RNA synthesis during viral replication.
基因功能參考文獻：
1. NS3 interaction with NS4B is required for Hepatitis GB virus B replication. PMID: 20701941
2. signal peptide peptidase cleavage of GB virus B core protein is required for productive infection in vivo PMID: 16882659
3. GBV-B NS3/4A protease specifically cleaves MAVS and dislodges it from mitochondria, thereby disrupting its function as a RIG-I adaptor and blocking downstream activation of both interferon regulatory factor 3 and nuclear factor kappa B. [NS3/4A] PMID: 17093192
4. GBV-B NS5A fails to inhibit Ras-Erk signalling, but elevates beta catenin-dependent transcription via PI3K activation. This differnetial effect contributes to differences in the natural history of the Hepatitis C and GB viruses. PMID: 18632962
亞細胞定位：

[Core protein]: Virion. Host cytoplasm. Host lipid droplet.; [Envelope glycoprotein E1]: Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum membrane; Single-pass type I membrane protein.; [Envelope glycoprotein E2]: Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host lipid droplet.; [p13]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Protease NS2]: Host endoplasmic reticulum membrane; Multi-pass membrane protein. Host lipid droplet.; [Serine protease/helicase NS3]: Host endoplasmic reticulum membrane; Peripheral membrane protein.; [Non-structural protein 4A]: Host endoplasmic reticulum membrane; Single-pass type I membrane protein.; [Non-structural protein 5A]: Host endoplasmic reticulum membrane; Peripheral membrane protein. Host cytoplasm, host perinuclear region. Host mitochondrion. Host cytoplasm. Host nucleus. Host lipid droplet.; [RNA-directed RNA polymerase]: Host cytoplasm. Host endoplasmic reticulum membrane; Single-pass type IV membrane protein.
數據庫鏈接：

KEGG: vg:1403460