Recombinant Escherichia coli Transcription termination factor Rho (rho)

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Stock}

中文名稱：

大腸桿菌rho重組蛋白
品名簡稱：

Recombinant E.coli rho protein
貨號：

CSB-RP166274Ba
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP166274Ba could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) rho.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP166274Ba could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) rho.
其他：

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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

rho
Uniprot No.：

P0AG30
別名：

rho; nitA; psuA; rnsC; sbaA; tsu; b3783; JW3756; Transcription termination factor Rho; EC 3.6.4.-; ATP-dependent helicase Rho
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length
來源：

E.coli
分子量：

51.0kDa
表達區域：

1-419aa
氨基酸序列

MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMKRS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPase which utilizes all four ribonucleoside triphosphates as substrates.
基因功能參考文獻：
1. Our findings further show that the RNA sequence specificity used for guiding Rho-dependent termination derives in part from an intrinsic ability of the motor to couple the recognition of pyrimidine patterns in nascent transcripts to RNA loading and activity. PMID: 27821776
2. results, together with existing data, support a model in which the connector segment plays a hitherto overlooked role in the regulation of Rho-dependent termination PMID: 28559482
3. Translational control and Rho-dependent transcription termination are intimately linked in riboswitch regulation. PMID: 28520932
4. NusG acts as both a positive and negative regulator of Rho in the course of the bacterial transcription termination. (Review) PMID: 27023849
5. Finally, identification of the NusG binding sites on the Rho hexamer led us to conclude that the former exerts its effect allosterically. PMID: 27605667
6. Rho inhibition leads to RNA polymerase readthrough, which in principle could displace H-NS from the DNA, thus leading to transcriptional derepression of H-NS-silenced genes. PMID: 24499790
7. Rho binds C-rich unstructured nascent RNA (high C/G ratio) prior to its ATP-dependent dissociation of transcription complexes PMID: 23207917
8. Tthe in vivo Rho-dependent termination process is kinetically controlled. PMID: 22442304
9. Here the authors provide direct evidence that the beta-sheet bundle of the C-terminal domain of NusG (NusG-CTD) has the binding determinants for Rho. PMID: 21040729
10. The authors mutated E211, R366, R212, and D265, and characterized the resulting proteins for oligomerization, ligand binding and RNA-dependent ATP hydrolysis that support the existing model of ATP hydrolysis. PMID: 20950626
11. Global hydrogen-deuterium exchange indicate net mass differences of about 15 Da after 1 h of exchange in the presence--versus in the absence--of the ligand MgATP or the RNA poly(C). PMID: 20708016
12. Results indicate that all three Rho catalytic sites must be filled with substrate to achieve the enhanced catalytic rate, both in pre-steady-state and in steady-state hydrolysis. PMID: 15703177
13. Results reinforce the importance of catalytic cooperativity in normal Rho function and suggest that several protein conformations exist along the catalytic pathway. PMID: 15703178
14. findings show that transcription termination of fimE is Rho dependent and is suppressed in a rho mutant or by bicyclomycin treatment when fimE mRNA is expressed by the fimE gene, either from a recombinant plasmid or in its native chromosomal location PMID: 16321930
15. mutant forms of Rho were defective in transcriptional termination, suggesting that those residues play an important role in the activation of Rho by bound RNA PMID: 16908525
16. interactions in the primary RNA binding domain and in the Q-loop are mandatory for RNA release to occur and propose that the interactions in the primary RNA binding modulate most of the other functions of Rho allosterically PMID: 17599352
17. results reveal Rho factor as a global regulator of gene expression under normal growth conditions; it serves role of maintaining transcriptional boundaries; Rho termination, supported by NusA & NusG, is required to suppress toxic activity of foreign genes PMID: 18487194
18. ADP but not P(i) dissociation contributes to rate limitation for Escherichia coli Rho PMID: 19837672
19. These data show that Rho forms uneven productive interactions with the track nucleotides and disrupts RNA-DNA duplexes in a succession of large (approximately 7-nucleotide-long) discrete steps triggered by 2'-hydroxyl activation events. PMID: 19915588
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蛋白家族：

Rho family
數據庫鏈接：

KEGG: ecj:JW3756

STRING: 316385.ECDH10B_3972