Recombinant Escherichia coli S-ribosylhomocysteine lyase (luxS)

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中文名稱：

大腸桿菌luxS重組蛋白
品名簡稱：

Recombinant E.coli luxS protein
貨號：

CSB-EP541717ENT
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

luxS
Uniprot No.：

P45578
別名：

luxS; ygaG; b2687; JW2662; S-ribosylhomocysteine lyase; EC 4.4.1.21; AI-2 synthesis protein; Autoinducer-2 production protein LuxS
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

23.3 kDa
表達區域：

2-171aa
氨基酸序列

PLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPNKEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVADAWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELALPKEKLQELHI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).
基因功能參考文獻：
1. study demonstrated that luxS affects the expression of flagella and Stx2e toxin, 2 important STEC virulence factors PMID: 24848979
2. LuxS contributes to virulence in avian pathogenic Escherichia coli O78:K80:H9. PMID: 23958403
3. Proteomic profiling of luxS mutants showed only a few proteins were differentially expressed and those that were altered suggested a metabolic rather than communication role for the E. coli luxS gene product. PMID: 23651217
4. Deletion of the luxS gene reduced the bacterial virulence by 31.5-fold in ducklings. PMID: 23046700
5. Polyphosphate degradation in stationary phase triggers biofilm formation via LuxS quorum sensing system in Escherichia coli. PMID: 23226268
6. Pfs and LuxS synthesize AI-2 in vitro from S-ribosylhomocysteine. PMID: 23236852
7. luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12. PMID: 20875395
8. These data are consistent with the function of LuxS as an important metabolic enzyme but appear not to support the role of AI-2 as a true signal molecule for E. coli W3110 under the investigated conditions. PMID: 16321939
9. The active form of LuxS contains a metal-bound water and a thiolate ion at Cys-83, consistent with the proposed roles of the metal ion (Lewis acid) and Cys-83 (general acid/base) during catalysis. PMID: 17014073
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蛋白家族：

LuxS family
數據庫鏈接：

KEGG: ecj:JW2662

STRING: 316385.ECDH10B_2854