E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
問答及客戶評論
■ 常見問題解答
Q:
I am looking to buy the protein RPI-A with an N-terminal HIS-tag. As I work with highly sensitive aptamer tools, I need to know the exact sequence before I buy. Is it only the protein plus 6xHIS, or is there a linker contained as well? If so, do you know if it impacts the structure?
A:
Thanks for your inquiry. Recombinant Escherichia coli Ribose-5-phosphate isomerase A(rpiA) Express Region: 1-219aa; Full length protein. Tag information:We are not sure you need C terminal 6*his tag or N terminal 6*his tag. We will try to link to the tag you want in prior, if we can't provide it, we could provide other tags. ( The success rate is 95.25%) Sequence:
If you need 6*his, there will be no linker. If you need the fusion protein with other tags, there will be linkers. Usually it doesn't impact the structurer. Shipping format: The default delivery form is liquid form. If you have demand for lyophilized form, please remark this requirement when placing order. Optional service: Do you want aseptic processing and/or endotoxin removal for this protein (recommended for cell culture or in vivo use)? With our endotoxin removal service we guarantee the endotoxin level to be less than 0.1ng/ug (1EU ug). Both aseptic processing and endotoxin removal services are free of charge however they must be requested when the order is placed. Western Blot service: We could offer Western Blot service for tag antibody free of charge. If you have demand, please remark this information when placing the order. Shipping condition: Our proteins are routinely shipped on ice packs.