Recombinant Escherichia coli Peptidyl-tRNA hydrolase (pth)

中文名稱：

大腸桿菌pth重組蛋白
貨號：

CSB-YP358958ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌pth重組蛋白
貨號：

CSB-EP358958ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌pth重組蛋白
貨號：

CSB-BP358958ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌pth重組蛋白
貨號：

CSB-MP358958ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

pth
Uniprot No.：

P0A7D1
別名：

pth; b1204; JW1195; Peptidyl-tRNA hydrolase; PTH; EC 3.1.1.29
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-194
氨基酸序列

MTIKLIVGLA NPGAEYAATR HNAGAWFVDL LAERLRAPLR EEAKFFGYTS RVTLGGEDVR LLVPTTFMNL SGKAVAAMAS FFRINPDEIL VAHDELDLPP GVAKFKLGGG HGGHNGLKDI ISKLGNNPNF HRLRIGIGHP GDKNKVVGFV LGKPPVSEQK LIDEAIDEAA RCTEMWFTDG LTKATNRLHA FKAQ
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death.
基因功能參考文獻：
1. Study identifies the amino acid residues involved in the tRNA moiety recognition and also revealed the mechanism by which Pth accepts the diverse sequences of the elongator-tRNAs as substrate components. PMID: 22923517
2. crystals of peptidyl-tRNA hydrolase belonged to the hexagonal space group P6(1), with unit-cell parameters a = b = 55.1, c = 413.1 A PMID: 22139168
3. Possible mechanism for the suppression of thermosensitivity of pth(Ts) mutants through tRNALys overproduction. PMID: 16540595
亞細胞定位：

Cytoplasm.
蛋白家族：

PTH family
數據庫鏈接：

KEGG: ecj:JW1195

STRING: 316385.ECDH10B_1257