Recombinant Escherichia coli NADH dehydrogenase (ndh)

中文名稱：

大腸桿菌ndh重組蛋白
貨號：

CSB-YP355519ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌ndh重組蛋白
貨號：

CSB-EP355519ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌ndh重組蛋白
貨號：

CSB-EP355519ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌ndh重組蛋白
貨號：

CSB-BP355519ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌ndh重組蛋白
貨號：

CSB-MP355519ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ndh
Uniprot No.：

P00393
別名：

ndh; b1109; JW1095NADH dehydrogenase; EC 1.6.99.3
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-434
氨基酸序列

TTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL LHEVATGSLD EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR DEKGELLVPE RKIAYDTLVM ALGSTSNDFN TPGVKENCIF LDNPHQARRF HQEMLNLFLK YSANLGANGK VNIAIVGGGA TGVELSAELH NAVKQLHSYG YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG VRVLTQTMVT SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN GKPLKNYQYK DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL YRMHQIALHG YFKTGLMMLV GSINRVIRPR LKLH
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Alternative, nonproton pumping NADH:quinone oxidoreductase that delivers electrons to the respiratory chain by oxidation of NADH and reduction of quinones. Utilizes NADH exclusively, and electron flow from NADH to ubiquinone does not generate an electrochemical gradient.; It may also contribute to copper homeostasis and bacterial oxidative protection. Shows cupric reductase activity, and catalyzes the reduction of Cu(2+) to Cu(+) with NADH as electron donor. Exhibits Cu(2+) reductase activity in the presence of either FAD or quinone, but is unable to reduce Fe(3+). Contains thiolate-bound copper.
基因功能參考文獻：
1. Because of the specific stabilization of substrate complexes of EcNDH-2 immobilized on electrodes, it was possible to demonstrate the presence of two distinct substrate binding sites for NADH and the quinone and to identify a bound semiprotonated quinol as a catalytic intermediate. PMID: 27109164
2. Improved poly(3-hydroxybutyrate) production in Escherichia coli by inactivation of cytochrome bd-II oxidase or/and NDH-II dehydrogenase in low efficient respiratory chains PMID: 25281801
3. The paper reports that the maintenance of NDH-2 activity and ndh expression in the stationary phase when cells were grown in media containing at least 37 mM phosphate. PMID: 18492062
亞細胞定位：

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

NADH dehydrogenase family
數據庫鏈接：

KEGG: ecj:JW1095

STRING: 316385.ECDH10B_1181