Recombinant Escherichia coli N-acetylglucosamine repressor (nagC)

中文名稱：

大腸桿菌nagC重組蛋白
貨號：

CSB-YP365237ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌nagC重組蛋白
貨號：

CSB-EP365237ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌nagC重組蛋白
貨號：

CSB-EP365237ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌nagC重組蛋白
貨號：

CSB-BP365237ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌nagC重組蛋白
貨號：

CSB-MP365237ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

nagC
Uniprot No.：

P0AF20
別名：

nagC; nagR; b0676; JW0662; N-acetylglucosamine repressor
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-406
氨基酸序列

MTPGGQAQIG NVDLVKQLNS AAVYRLIDQY GPISRIQIAE QSQLAPASVT KITRQLIERG LIKEVDQQAS TGGRRAISIV TETRNFHAIG VRLGRHDATI TLFDLSSKVL AEEHYPLPER TQQTLEHALL NAIAQFIDSY QRKLRELIAI SVILPGLVDP DSGKIHYMPH IQVENWGLVE ALEERFKVTC FVGHDIRSLA LAEHYFGASQ DCEDSILVRV HRGTGAGIIS NGRIFIGRNG NVGEIGHIQV EPLGERCHCG NFGCLETIAA NAAIEQRVLN LLKQGYQSRV PLDDCTIKTI CKAANKGDSL ASEVIEYVGR HLGKTIAIAI NLFNPQKIVI AGEITEADKV LLPAIESCIN TQALKAFRTN LPVVRSELDH RSAIGAFALV KRAMLNGILL QHLLEN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as a repressor of the nagEBACD operon and acts both as an activator and a repressor for the transcription of the glmSU operon.
基因功能參考文獻：
1. This work identifies four amino acids in the linker region which allow NagC to distinguish between a C/G and an A/T bp at positions +/-11 of the operators. PMID: 25452338
2. Mutations in NagC, at positions which form a structural motif resembling a glucose binding site in Mlc, produce permanently repressing forms of NagC, suggesting that this motif forms a N-acetylglucosamine 6-phosphate binding site in NagC. PMID: 18067539
3. Results show that changes at either of two Mlc operators upstream of ptsG are sufficient to lose regulation by Mlc but that both types of changes in both operators are necessary to convert ptsG to a gene regulated by NagC. PMID: 18469102
4. NagC binds to a single, high-affinity site overlapping the promoter of galP. PMID: 19007420
蛋白家族：

ROK (NagC/XylR) family
數據庫鏈接：

KEGG: ecj:JW0662

STRING: 316385.ECDH10B_0741