Recombinant Escherichia coli Multidrug transporter MdfA (mdfA), partial

中文名稱：

大腸桿菌mdfA重組蛋白
貨號：

CSB-YP365221ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌mdfA重組蛋白
貨號：

CSB-EP365221ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌mdfA重組蛋白
貨號：

CSB-EP365221ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌mdfA重組蛋白
貨號：

CSB-BP365221ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌mdfA重組蛋白
貨號：

CSB-MP365221ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

mdfA
Uniprot No.：

P0AEY8
別名：

mdfA; cmlA; cmr; b0842; JW0826; Multidrug transporter MdfA; Chloramphenicol resistance pump Cmr
種屬：

Escherichia coli (strain K12)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. Overexpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na(+) or K(+).
基因功能參考文獻：
1. MdfA has exceptional promiscuous capabilities not only in its interaction with a plethora of chemically dissimilar compounds, but also concerning the various faces of its modes of energy consumption. [review] PMID: 28951231
2. Crystallization and X-ray diffraction analysis of the multidrug-resistance transporter MdfA from Escherichia coli has been reported. PMID: 28695852
3. The MdfA structure consists of four pseudo-symmetrical, three-helix repeats, consistent with the typical structure of an MFS transporter. PMID: 26238402
4. The mdfA gene can function as transporter responsible for multidrug resistance, and also mediated the synergistic function with its surrounding genes in conjugative plasmids. PMID: 24684286
5. GlpG is able to recognize and cleave truncated forms of MdfA but not the intact protein. PMID: 19919105
6. results show that MdfA confers extreme alkaline pH resistance and allows the growth of transformed cells under conditions that are close to those used normally by alkaliphiles (up to pH 10) by maintaining a physiological internal pH PMID: 15371593
7. results suggest that in the absence of specific chloramphenicol selection pressure, the cmlA gene is maintained by virtue of gene linkage to genes encoding resistance to antimicrobials that are currently approved for use in food animals PMID: 15668031
8. Arg112 is important for multidrug transport by MdfA. PMID: 16274234
9. properties of MdfA including its multidrug recognition capacity, proton recognition determinants, aspects of energy utilization, and physiological role [review] PMID: 17114895
10. the Mdr-binding pocket of MdfA is conformationally sensitive PMID: 19808670
11. In this study, we got an evidence that MdfA is a chloramphenicol/H+ antiporter. PMID: 9644262
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亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

Major facilitator superfamily, MdfA family
數據庫鏈接：

KEGG: ag:CAA69997

STRING: 316385.ECDH10B_0911