Recombinant Escherichia coli Flagellar motor switch protein FliG (fliG)
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中文名稱：大腸桿菌fliG重組蛋白
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貨號(hào)：CSB-EP364722ENV-B
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說明書：
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規(guī)格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產(chǎn)品詳情
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純度：>85% (SDS-PAGE)
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基因名：fliG
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Uniprot No.：
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別名：fliG; fla; AII.2; fla; BII; b1939; JW1923Flagellar motor switch protein FliG
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種屬：Escherichia coli (strain K12)
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蛋白長度：full length protein
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表達(dá)區(qū)域：1-331
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氨基酸序列MSNLTGTDKS VILLMTIGED RAAEVFKHLS QREVQTLSAA MANVTQISNK QLTDVLAEFE QEAEQFAALN INANDYLRSV LVKALGEERA ASLLEDILET RDTASGIETL NFMEPQSAAD LIRDEHPQII ATILVHLKRA QAADILALFD ERLRHDVMLR IATFGGVQPA ALAELTEVLN GLLDGQNLKR SKMGGVRTAA EIINLMKTQQ EEAVITAVRE FDGELAQKII DEMFLFENLV DVDDRSIQRL LQEVDSESLL IALKGAEQPL REKFLRNMSQ RAADILRDDL ANRGPVRLSQ VENEQKAILL IVRRLAETGE MVIGSGEDTY V
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蛋白標(biāo)簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng)：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情
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功能：FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.
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基因功能參考文獻(xiàn)：
- The results indicate a copy number of ~25 for FliG in the complex that regulates the direction of flagellar rotation. PMID: 28259628
- FliG exists predominantly as a monomer in solution but only as domain-swapped polymers in assembled flagellar motors. PMID: 26854663
- The biophysical characterization of this protein is determined. PMID: 24174548
- FliG molecules with pairs of Cys residues in the middle domain formed disulfide-linked dimers and larger multimers, so the middle domains of adjacent subunits are in fairly close proximity and putting constraints on the relative orientation of the domains PMID: 16077109
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亞細(xì)胞定位：Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body.
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蛋白家族：FliG family
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數(shù)據(jù)庫鏈接：
KEGG: ecj:JW1923
STRING: 316385.ECDH10B_2081
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