Recombinant Escherichia coli DNA protection during starvation protein (dps)

中文名稱：

大腸桿菌dps重組蛋白
貨號：

CSB-YP364693ENV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌dps重組蛋白
貨號：

CSB-EP364693ENV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌dps重組蛋白
貨號：

CSB-EP364693ENV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌dps重組蛋白
貨號：

CSB-BP364693ENV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌dps重組蛋白
貨號：

CSB-MP364693ENV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

dps
Uniprot No.：

P0ABT2
別名：

dps; pexB; vtm; b0812; JW0797DNA protection during starvation protein; EC 1.16.-.-
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-167
氨基酸序列

STAKLVKSK ATNLLYTRND VSDSEKKATV ELLNRQVIQF IDLSLITKQA HWNMRGANFI AVHEMLDGFR TALIDHLDTM AERAVQLGGV ALGTTQVINS KTPLKSYPLD IHNVQDHLKE LADRYAIVAN DVRKAIGEAK DDDTADILTA ASRDLDKFLW FIESNIE
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

During stationary phase, binds the chromosome non-specifically, forming a highly ordered and stable dps-DNA co-crystal within which chromosomal DNA is condensed and protected from diverse damages. It protects DNA from oxidative damage by sequestering intracellular Fe(2+) ion and storing it in the form of Fe(3+) oxyhydroxide mineral, which can be released after reduction. One hydrogen peroxide oxidizes two Fe(2+) ions, which prevents hydroxyl radical production by the Fenton reaction. Dps also protects the cell from UV and gamma irradiation, iron and copper toxicity, thermal stress and acid and base shocks. Also shows a weak catalase activity.
基因功能參考文獻：
1. A double-alanine mutant of E. coli Dps protein is unable to assemble into a cage due to disruption of key protein-protein interactions. Randomization of residues surrounding the double mutation afforded a repacked interface and proteins with recovered cage formation. PMID: 29276894
2. three new candidates of E. coli nucleoid, namely DNA-binding protein from starved cells (Dps), host factor for phage Qbeta (Hfq) and suppressor of td(-) phenotype A (StpA) PMID: 26208826
3. Oxidative stress response proteins such as AhpF, Dps, and Fur, all of which belong to the oxyR regulon, were upregulated in Escherichia coli isolates from mice fed the lactose-rich diet. PMID: 22427493
4. study investigated the role key protein-protein interactions play in the assembly and stability of the DPS mini-ferritin PMID: 21898653
5. Unexpectedly, the authors found that dps expression is directly repressed by Mn(2+)-MntR. PMID: 21239586
6. In contrast, cells lacking Dps exhibited increased cellular copper concentrations compared to their wild-type parent. PMID: 19231146
7. We describe Escherichia coli survival following neutrophil phagocytosis among three mutants deleted respectively for araB, dps or oxyR. PMID: 15507758
8. Data suggest thatDps acts as a checkpoint during oxidative stress to reduce initiations, providing an opportunity for mechanisms to repair oxidative DNA damage. PMID: 18284581
9. Fis and H-NS selectively repress the dps promoter, preventing transcription initiation by RNA polymerase. PMID: 18452510
10. Dps protected DNA from acid damage by binding to DNA. Dps maintains or forms alpha-helices that are important for Dps-DNA complex formation. PMID: 18922164
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亞細胞定位：

Cytoplasm, nucleoid.
蛋白家族：

Dps family
數據庫鏈接：

KEGG: ecj:JW0797

STRING: 316385.ECDH10B_0880