Recombinant Escherichia coli DNA polymerase III subunit epsilon (dnaQ)

中文名稱：

大腸桿菌dnaQ重組蛋白
貨號(hào)：

CSB-YP360933ENV
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

大腸桿菌dnaQ重組蛋白
貨號(hào)：

CSB-EP360933ENV
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

大腸桿菌dnaQ重組蛋白
貨號(hào)：

CSB-EP360933ENV-B
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌dnaQ重組蛋白
貨號(hào)：

CSB-BP360933ENV
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

大腸桿菌dnaQ重組蛋白
貨號(hào)：

CSB-MP360933ENV
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

dnaQ
Uniprot No.：

P03007
別名：

dnaQ; mutD; b0215; JW0205; DNA polymerase III subunit epsilon; EC 2.7.7.7
種屬：

Escherichia coli (strain K12)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-243
氨基酸序列

MSTAITRQIV LDTETTGMNQ IGAHYEGHKI IEIGAVEVVN RRLTGNNFHV YLKPDRLVDP EAFGVHGIAD EFLLDKPTFA EVADEFMDYI RGAELVIHNA AFDIGFMDYE FSLLKRDIPK TNTFCKVTDS LAVARKMFPG KRNSLDALCA RYEIDNSKRT LHGALLDAQI LAEVYLAMTG GQTSMAFAME GETQQQQGEA TIQRIVRQAS KLRVVFATDE EIAAHEARLD LVQKKGGSCL WRA
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease. Contacts both the beta sliding clamp (dnaN) and the polymerase subunit (dnaE), stabilizing their interaction.
基因功能參考文獻(xiàn)：
1. Loss of the 3' proofreading exonuclease activity of the replicative DNA polymerase III greatly elevates quasipalindrome-associated mutagenesis. PMID: 28605670
2. evidence that hydrophobic interactions have a predominant contribution to the stability of the complex and that the association of epsilon186 with theta stabilized epsilon186 PMID: 15459336
3. Data shows that the proofreading domain of epsilon subunit is connected to the alpha subunit via a flexible linker peptide comprising over 20 residues. PMID: 18663010
數(shù)據(jù)庫(kù)鏈接：

KEGG: ecj:JW0205

STRING: 316385.ECDH10B_0196