Recombinant Enterobacteria phage T4 Endonuclease II (denA)

中文名稱：

Recombinant Enterobacteria phage T4 Endonuclease II(denA)
貨號：

CSB-YP357009EDZ
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Enterobacteria phage T4 Endonuclease II(denA)
貨號：

CSB-EP357009EDZ
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Enterobacteria phage T4 Endonuclease II(denA)
貨號：

CSB-EP357009EDZ-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Enterobacteria phage T4 Endonuclease II(denA)
貨號：

CSB-BP357009EDZ
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Enterobacteria phage T4 Endonuclease II(denA)
貨號：

CSB-MP357009EDZ
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

denA
Uniprot No.：

P07059
別名：

denA; Endonuclease II; EC 3.1.21.8
種屬：

Enterobacteria phage T4 (Bacteriophage T4)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-144
氨基酸序列

MGRKAHFIMK EIATEYSFIK YTELELDDNG SIKQLSIPNK YNVIYAIAIN DELVYIGKTK NLRKRINYYR TAINRKDKTS DSTKSALIHS ALKEGSKVEF YARQCFNLSM TNELGTMTIA TIDLEEPLFI KLFNPPWNIQ HKKK
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Contributes to the degradation of host DNA, permitting the scavenging of host-derived nucleotides for phage DNA synthesis. Sequence-specific endonuclease. Catalyzes nicking of the bottom strand of double-stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Does not cleave native phage DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
基因功能參考文獻：
1. EndoII forms a striking X-shaped tetrameric structure composed as a dimer of dimers, with a protruding hairpin domain not present in UvrC or I-TevI providing most of the dimerization and tetramerization interfaces. PMID: 20156453
2. The conserved glycine (G49) and arginine (R57) were essential for normal sequence recognition. PMID: 18539732
數(shù)據(jù)庫鏈接：

KEGG: vg:1258710