Recombinant Drosophila melanogaster Cryptochrome-1 (cry), partial

中文名稱：

Recombinant Drosophila melanogaster Cryptochrome-1(cry) ,partial
貨號：

CSB-YP006005DLU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Drosophila melanogaster Cryptochrome-1(cry) ,partial
貨號：

CSB-EP006005DLU
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Drosophila melanogaster Cryptochrome-1(cry) ,partial
貨號：

CSB-EP006005DLU-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Drosophila melanogaster Cryptochrome-1(cry) ,partial
貨號：

CSB-BP006005DLU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Drosophila melanogaster Cryptochrome-1(cry) ,partial
貨號：

CSB-MP006005DLU
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

cry
Uniprot No.：

O77059
別名：

cry; CG3772Cryptochrome-1; DmCRY1; dcry; Blue light photoreceptor
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that cry is in the input pathway of magnetic sensing.
基因功能參考文獻：
1. photo-induced electron transfer reactions in Drosophila melanogaster cryptochrome are indeed influenced by magnetic fields of a few millitesla. PMID: 28176875
2. Substitutions of four key Trp residues to redox-active tyrosine and redox-inactive phenylalanine tune the light sensitivity of dCRY photoreduction, conformational activation, cellular stability, and targeted degradation of the clock protein timeless PMID: 29581265
3. we identify the circadian blue-light photoreceptor CRYPTOCHROME as a molecular regulator of Prolonged Morning Wakefulness (PMW), and propose a model in which the Drosophila nervous system integrates information encoding temperature, light, and time to dynamically control when sleep is initiated. Our results provide a platform to investigate how environmental inputs co-ordinately regulate sleep plasticity. PMID: 28084307
4. The findings of this article for the first time define CRY expression in Drosophila peripheral tissues and reveal that CRY acts together with K(+) channels to maintain passive membrane properties in a non-clock-containing peripheral tissue independent of light. PMID: 28781048
5. Changes in active site histidine hydrogen bonding trigger cryptochrome activation. PMID: 27551082
6. CRY is not stabilized by interaction with the kinase Shaggy (SGG), the GSK-3 beta fly orthologue. PMID: 26741981
7. our studies provided novel evidence that the circadian clock gene, dCry, plays an essential role in heart morphogenesis and function. PMID: 26348211
8. Using time-resolved and steady-state optical spectroscopy, we studied the mechanism of light-induced radical-pair formation and decay, and the photoreduction of the FAD cofactor. PMID: 25879256
9. The results of this study concluded that the E oscillators are the targets of light input via CRY and the visual system and are required for normal light entrainment. PMID: 25878285
10. The Cry leucine-histidine substitution is common in Drosophila, with both alleles at intermediate frequencies across 27 populations surveyed in Europe, irrespective of latitude. PMID: 24475129
11. Both oxidized and reduced forms of dCRY are capable of photosignaling. PMID: 24379403
12. Flavin reduction activates Drosophila cryptochrome. PMID: 24297896
13. Our findings indicate that CRY compensates for limited light sensitivity in vivo by photon integration over extraordinarily long times, and point to select circadian pacemaker neurons as having important roles. PMID: 23874218
14. dampens temperature-induced PERIOD oscillations in dorsal clock neurons PMID: 23333312
15. Study reports crystal structures of full-length dCRY, a dCRY loop deletion construct, and the photolyase homology region of mouse CRY1 (mCRY1). PMID: 23746849
16. Data indicate that wild-type flies show greatly enhanced magnitude of phase shift, but cryptochrome (CRY)-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner. PMID: 22476772
17. Study reports that increased nighttime activity of Clk mutants is mediated by high levels of the circadian photoreceptor CRYPTOCHROME (CRY) in large ventral lateral neurons (l-LN(v)s). PMID: 22581798
18. Drosophila photolyases contain F0 as the second chromophore; within the limitation of the current assay, neither plant nor animal cryptochromes seem to carry a second chromophore. PMID: 22175817
19. 2.3-A resolution crystal structure of Drosophila CRY with an intact C terminus PMID: 22080955
20. Data show that CRY is a key component for the preferential entrainment to light. PMID: 21135155
21. study shows the CRY-mediated light response requires a flavin redox-based mechanism and depends on potassium channel conductance but is independent of the classical circadian CRY-TIMELESS interaction PMID: 21385718
22. CRY's photolyase homology domain is sufficient for light detection and phototransduction, whereas the carboxyl-terminal domain regulates CRY stability, CRY-TIM interaction, and circadian photosensitivity PMID: 15178801
23. Cryb mutant flies exhibited free-running rhythms with two rhythmic components. Drosophila locomotor rhythms may be driven by two separate PER-dependent clocks, responding differentially to constant light. PMID: 15183277
24. show a strong interaction between Veela and cryptochrome genetic variants, demonstrating that the Jetlag, Timeless, and Cry proteins function in the same pathway PMID: 17068124
25. Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons PMID: 18399544
26. CRY is not a core component, but an exclusive photoreceptor in the cuticle deposition rhythm. PMID: 18539772
27. the first genetic evidence for a Cry-based magnetosensitive system in any animal PMID: 18641630
28. Spatial and cricadian regulation of cry in Drosophila are reported. PMID: 18663236
29. cry is expressed in a subset of circadian oscillator neurons in the Drosophila CNS. PMID: 18663237
30. After illumination, Jetlag protein induces massive degradation of Cry as part of the resetting process of the circadian clock in reaction to light. PMID: 19185492
31. These results suggest that cry, Rh1, Rh5 and Rh6 are essential for circadian photoentrainment to green and yellow light. PMID: 19398933
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亞細胞定位：

Cytoplasm. Cytoplasm, perinuclear region. Nucleus.
蛋白家族：

DNA photolyase class-1 family
組織特異性：

Expressed at higher levels in the head than in body and it is more expressed in antennae than in legs, wings and mouth appendages. Prominent expression is seen in cells of the lateral brain, which are close to or coincident with the clock neurons. Abundan
數(shù)據(jù)庫鏈接：

KEGG: dme:Dmel_CG3772

STRING: 7227.FBpp0083150

UniGene: Dm.4579