Recombinant DNA polymerase (pol)

中文名稱：

Recombinant DNA polymerase(pol)
貨號：

CSB-YP301888TDF
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant DNA polymerase(pol)
貨號：

CSB-EP301888TDF
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant DNA polymerase(pol)
貨號：

CSB-EP301888TDF-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant DNA polymerase(pol)
貨號：

CSB-BP301888TDF
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant DNA polymerase(pol)
貨號：

CSB-MP301888TDF
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

pol
Uniprot No.：

P74918
別名：

polDNA polymerase; EC 2.7.7.7; Pol Tfu) [Cleaved into: Endonuclease PI-TfuI; EC 3.1.-.-; Tfu pol-1 intein); Endonuclease PI-TfuII; EC 3.1.-.-; Tfu pol-2 intein)]
種屬：

Thermococcus fumicolans
蛋白長度：

full length protein
表達區域：

1-406
氨基酸序列

MILDTDYITE DGRPVIRVFK KENGEFKIEY DRDFEPYIYA LLKDDSAIED VKKITASRHG TTVRVVRAGK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKMLAF DIETLYHEGE EFAEGPILMI SYADEEGARV ITWKKIDLPY VDVVSTEKEM IKRFLKVVKE KDPDVLITYN GDNFDFAYLK KRSEKLGVKF ILGRDGSEPK IQRMGDRFAV EVKGRIHFDL YPVIRHTINL PTYTLEAVYE AIFGQPKEKV YAEEIAQAWE TGEGLERVAR YSMEDAKVTY ELGREFFPME AQLSRLVGQS FWDVSRSSTG NLVEWYLLRK AYERNELAPN KPSGRELERR RGGYAGGYVK EPERGLWENI AYLDFR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

PI-TfuI recognizes and cleaves a minimal sequence of 16 base pairs (bp) on supercoiled DNA with either Mn(2+) or Mg(2+) as cofactor. It cleaves linear DNA only with Mn(2+) and requires a 19-bp minimal recognition sequence.; PI-TfuII is a highly active homing endonuclease using Mg(2+) as cofactor. Its minimal recognition and cleavage site is 21 bp long either on linear or circular DNA substrates. Its endonuclease activity is strongly inhibited by the 3' digestion product, which remains bound to the enzyme after the cleavage reaction.
蛋白家族：

DNA polymerase type-B family